Effect of environmental conditions on extracellular lipases production and fungal morphology from <i>Aspergillus niger</i> MYA 135

Colin, Verónica Leticia; Baigorí, Mario Domingo; Pera, Licia María

doi:10.1002/jobm.200900162

Cited by 18 publications

(20 citation statements)

References 22 publications

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“…Thus, as it was mentioned before, the addition of 1 g l -1 FeCl 3 to the BM encourages a scarcely branched mycelium and increases the production of both a mycelium-bound transesterification lipase activity (Colin et al 2011) and an emulsifier compound (Colin et al 2010a). Interestingly, in the same medium supplemented with 2% olive oil it was also observed that a specific extracellular hydrolytic lipase activity was increased by 6.6 fold after four days of incubation compared to the control (Colin et al 2010b). Finally, another application is the enhancement of protoplast formation in Phaffia rhodozyma .…”

Section: Discussionmentioning

confidence: 94%

Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications

2013

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Current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In this connection, this work explored new experimental procedures in order to quantitatively check the potential of some culture conditions to induce a determined fungal morphology by altering both hyphal morphology and conidia adhesion capacity. The capacity of environmental conditions to modify hyphal morphology was evaluated by examining the influence of some culture conditions on the cell wall lytic potential of Aspergillus niger MYA 135. The relative value of the cell wall lytic potential was determined by measuring a cell wall lytic enzyme activity such as the mycelium-bound β-N-acetyl-D-glucosaminidase (Mb-NAGase). On the other hand, the quantitative value of conidia adhesion was considered as an index of its aggregation capacity. Concerning microscopic morphology, a highly negative correlation between the hyphal growth unit length (lHGU) and the specific Mb-NAGase activity was found (r = -0.915, P < 0.001). In fact, the environment was able to induce highly branched mycelia only under those culture conditions compatible with specific Mb-NAGase values equal to or higher than 190 U gdry.wt-1. Concerning macroscopic morphology, a low conidia adhesion capacity was followed by a dispersed mycelial growth. In fact, this study showed that conidia adhesion units per ml equal to or higher than 0.50 were necessary to afford pellets formation. In addition, it was also observed that once the pellet was formed the lHGU had an important influence on its final diameter. Finally, the biotechnological significance of such results was discussed as well.

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Section: Discussionmentioning

confidence: 94%

Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications

2013

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“…Effects of the culture conditions on biomass concentration from A. niger Previously, we reported on the effect of environmental factors in the biomass concentration from A. niger MYA 135, when cultured in the presence of 2% olive oil [9]. In the present work, fungal growth in the media without olive oil is also described (Fig.…”

Section: +2mentioning

confidence: 73%

“…In previous studies we found that extracellular lipases production from A. niger MYA 135 was strongly affected by the initial culture conditions [9]. However, naturally immobilized catalysts such as cell-bound microbial lipases have advantages over their extracellular counterparts, and have recently been receiving increased attention [14,15].…”

Section: Discussionmentioning

confidence: 99%

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Mycelium‐bound lipase production from Aspergillus niger MYA 135, and its potential applications for the transesterification of ethanol

Colin

Baigorí

Pera

2011

J. Basic Microbiol.

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The potential biotechnological applications of both constitutive and inducible lipase sources from Aspergillus niger MYA 135 were evaluated. To this end, the effect of environmental conditions on mycelium-bound lipase production from this strain was studied, when cultured either in the absence or presence of 2% olive oil. It was previously reported that mycelium-bound lipase from Aspergillus niger MYA 135 possess high stability in reaction mixtures containing ethanol; which could be especially important for their use in biodiesel synthesis. In this connection, the performance of the lipase sources produced in the transesterification of ethanol using p-nitrophenyl palmitate as acyl donor was also explored. Under our assay conditions, hydrolytic and synthetic activity of the mycelia produced in the absence or presence of olive oil were not highly correlated. While the hydrolytic activity was strongly increased by the addition of lipid to the culture medium, the best performance in the transesterification reactions of ethanol were associated with mycelia produced in absence of olive oil. Interestingly, the supplementation of the culture medium with Fe(+3) increased the transesterification activity by 71%, as compared to the activity previously reported for this strain. Therefore, the constitutive lipase sources from Aspergillus niger MYA 135 are considered to be promising for industrial biodiesel-fuel production.

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“…However, since the production, chemical compositions and functional properties of the bioemulsifiers can be strongly influenced by environmental factors, in this study the effect of culture conditions on the emulsifier production by Aspergillus niger MYA 135 was tested. In previous studies performed on this strain, it was found that the fungal growth and metabolites production such as citric acid (Pera and Callieri 1997) and extracellular lipases (Colin et al 2010) were strongly affected by the initial pH of the culture medium, the incubation temperature and by the presence of CaCl 2 or FeCl 3 in the medium. In this study, only at an initial pH 2.0 or in the BM supplemented with FeCl 3 the production of bioemulsifiers was significantly increased compared to the reference conditions (P \ 0.05) (Fig.…”

Section: Resultsmentioning

confidence: 98%

Bioemulsifier production by Aspergillus niger MYA 135: presumptive role of iron and phosphate on emulsifying ability

Colin

Baigorí

Pera

2010

World J Microbiol Biotechnol

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Microbial emulsifiers are compounds employed in primary mechanisms for bioremediation of petroleum and other hydrocarbon pollutants from the environment. Although emulsifiers of biological origin are produced by microorganisms generally in response to growth on hydrocarbons, Aspergillus niger MYA 135 produced a bioemulsifier during fermentation in a sucrose-based culture medium at an initial pH of 5.0 and at 30°C. The production of bioemulsifiers can be strongly influenced by environmental factors. In this connection, a study of the effect of initial pH, the incubation temperature and presence of CaCl 2 or FeCl 3 in the culture medium was conducted. Emulsification index was increased by 112 and 206% at an initial pH 2.0 or in medium supplemented with FeCl 3 , respectively. On the other hand, emulsifying ability of Aspergillus niger supernatants was detected during the exponential phase, suggesting that bioemulsifiers accumulation and microbial growth would be related. Interestingly, this study suggests that iron and/or phosphate ions would play a key role in maintaining the emulsifying ability. Finally, factorial design was also employed to study the effects of the initial pH, the presence of FeCl 3 and the concentration of KH 2 PO 4 on the emulsification index.

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Effect of environmental conditions on extracellular lipases production and fungal morphology from Aspergillus niger MYA 135

Cited by 18 publications

References 22 publications

Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications

Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications

Mycelium‐bound lipase production from Aspergillus niger MYA 135, and its potential applications for the transesterification of ethanol

Bioemulsifier production by Aspergillus niger MYA 135: presumptive role of iron and phosphate on emulsifying ability

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