Effect of enzymes (phytase and xylanase), probiotics (B. amyloliquefaciens) and their combination on growth performance and nutrient utilisation in Nile tilapia

Maas, Roel M.; Verdegem, M.C.J.; Debnath, Sudip; Marchal, Luc; Schrama, J.W.

doi:10.1016/j.aquaculture.2020.736226

Cited by 45 publications

(37 citation statements)

References 62 publications

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“…Certain strains that belong to B. amyloliquefaciens can synthesize many enzymes including amylase, cellulase, and xylanase, and therefore could potentially increase nutrient digestibility 54 – 56 . However, no beneficial effect of probiotics supplementation on nutrient digestion kinetics was observed in this study, except for improved fecal fat digestibility 57 .…”

Section: Discussioncontrasting

confidence: 74%

“…Ca is known precipitate accumulated free fatty acids, thereby Ca can enhance the accessibility of lipase to the emulsified lipids, leading to an increased lipase activity 62 , 63 . In this study, probiotics resulted in a higher fat digestibility (data shown in 57 ), which may be linked to this theorem.…”

Section: Discussionsupporting

confidence: 56%

“…Such supplementation would stimulate the breakdown of the polysaccharides into readily available oligomers and monomers for fermentation, increasing the production of beneficial VFA and improving the nutrient utilization. The enzyme supplementation in the present study did increase the NSP digestibility by approximately 29% (data not shown, 57 ). However, this increase did not go hand in hand with an increase in VFA levels, which remained unaffected by the enzyme supplementation (Fig.…”

Section: Discussionsupporting

confidence: 44%

“…A previous study showed that probiotics enhanced the species-species interaction network in the hindgut of sea cucumber, by increasing the network complexity (clustering co-efficiency and density), which was hypothesized to benefit the intestinal microbiota homeostasis 25 . Besides, we also found that probiotics supplementation enhanced the growth of tilapia with a smaller fraction of dietary energy going to maintenance 57 . A stable intestinal microbiota might be beneficial to the fish with high NSP diet inferences, thus reducing the energy requirement for maintenance.…”

Section: Discussionmentioning

confidence: 53%

“…The ADC in the stomach, proximal, middle and distal were determined on collected digesta in current study. The faecal ADCs were determined on faeces collected by settling columns during the growth trail (published in 57 ). Error bars indicate standard error of means.…”

Section: Resultsmentioning

confidence: 99%

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Exogenous enzymes and probiotics alter digestion kinetics, volatile fatty acid content and microbial interactions in the gut of Nile tilapia

Maas

Deng

Dersjant-Li³

et al. 2021

Sci Rep

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Sustainable aquafeed production requires fishmeal replacement, leading to an increasing use of plant-derived ingredients. As a consequence, higher levels of antinutritional substances, such as non-starch polysaccharides and phytate, are present in aquafeeds, with negative effects on fish performance, nutrient digestibility and overall gut health. To alleviate these negative effects, providing exogenous digestive enzymes and/or probiotics can be an effective solution. In this study, we tested the effect of dietary supplementation of enzymes (phytase and xylanase) and probiotics (three strains of Bacillus amyloliquefaciens) on nutrient digestion kinetics and volatile fatty acid content along the gut, and the distal gut microbiome diversity in Nile tilapia. Chyme volatile fatty content was increased with probiotic supplementation in the proximal gut, while lactate content, measured for the first time in vivo in fish, decreased with enzymes along the gut. Enzyme supplementation enhanced crude protein, Ca and P digestibility in proximal and middle gut. Enzymes and probiotics supplementation enhanced microbial interactions as shown by network analysis, while increased the abundance of lactic acid bacteria and Bacillus species. Such results suggest that supplementation with exogenous enzymes and probiotics increases nutrient availability, while at the same time benefits gut health and contributes to a more stable microbiome environment.

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Section: Discussioncontrasting

confidence: 74%

Section: Discussionsupporting

confidence: 56%

Section: Discussionsupporting

confidence: 44%

Section: Discussionmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

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