Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

Tzoneva, Rumiana; Stoyanova, Tihomira; Petrich, Annett; Popova, Desislava; Uzunova, Veselina; Momchilova, Albena; Chiantia, Salvatore

doi:10.1101/2020.03.09.983874

Cited by 9 publications

(1 citation statement)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, in order to quantify protein-protein interactions directly at the PM of living cells, we performed fluorescence fluctuation spectroscopy experiments under physiological conditions. Such experimental approaches (i.e., sF(C)CS and (cc)N&B) provide information regarding the oligomeric state, surface concentration, hetero-interactions and dynamics in complex biological systems [50, 55, 68, 76–78].…”

Section: Discussionmentioning

confidence: 99%

Influenza A M2 recruits M1 to the plasma membrane: a fluorescence fluctuation microscopy study

Petrich

Dunsing

Bobone

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Influenza A virus (IAV) is a respiratory pathogen that causes seasonal epidemics and occasional pandemics of severe illnesses with significant mortality. One of the most abundant proteins in IAV particles is the matrix protein 1 (M1), which is essential for the structural stability of the virus. M1 organizes virion assembly and budding at the plasma membrane (PM), where it can interact with other viral components and cellular membrane factors (i.e. lipids and host proteins). Of interest, the recruitment of M1 to the PM as well as its interaction with the other viral envelope proteins (hemagglutinin (HA), neuraminidase, matrix protein 2 (M2)) is controversially discussed in previous studies. Therefore, we used fluorescence fluctuation microscopy techniques (i.e. (cross-correlation) number and brightness, and scanning fluorescence cross-correlation spectroscopy) to quantify the oligomeric state of M1 and its interaction with other viral proteins in co-transfected as well as infected cells. Our results indicate that M1 is recruited to the PM by M2, as a consequence of the strong interaction between the two proteins. In contrast, only a weak interaction between M1 and HA was observed. M1-HA interaction occurred only in the case that M1 was already bound to the PM. We therefore conclude that M2 initiates the assembly of IAV by recruiting M1 to the PM, possibly allowing its further interaction with other viral proteins.

show abstract

Section: Discussionmentioning

confidence: 99%

Influenza A M2 recruits M1 to the plasma membrane: a fluorescence fluctuation microscopy study

Petrich

Dunsing

Bobone

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Influenza A virus hemagglutinin prevents extensive membrane damage upon dehydration

Iriarte-Alonso

Bittner

Chiantia

2021

Preprint

Self Cite

View full text Add to dashboard Cite

While the molecular mechanisms of virus infectivity are rather well known, the detailed consequences of environmental factors on virus biophysical properties are poorly understood. Seasonal influenza outbreaks are usually connected to the low winter temperature, but also to the low relative air humidity. Indeed, transmission rates increase in cold regions during winter. While low temperature must slow degradation processes, the role of low humidity is not clear. We studied the effect of relative humidity on a model of influenza A H1N1 virus envelope, a supported lipid bilayer containing the surface glycoprotein hemagglutinin (HA), which is present in the viral envelope in very high density. For complete cycles of hydration, dehydration and rehydration, we evaluate the membrane properties in terms of structure and dynamics, which we assess by combining confocal fluorescence microscopy, raster image correlation spectroscopy, line-scan fluorescence correlation spectroscopy and atomic force microscopy. Our findings indicate that the presence of HA prevents macroscopic membrane damage after dehydration. Without HA, fast membrane disruption is followed by irreversible loss of lipid and protein mobility. Although our model is principally limited by the membrane composition, the macroscopic effects of HA under dehydration stress reveal new insights on the stability of the virus at low relative humidity.

show abstract

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Mandal,

Brandt,

Tempra

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Cellular membranes are composed of lipids typically organized in a double-leaflet structure. Interactions between these two leaflets - often referred to as interleaflet coupling - play a crucial role in various cellular processes. Despite extensive study, the mechanisms governing such interactions remain incompletely understood. Here, we investigate the effects of interleaflet coupling from a specific point of view, i.e. by comparing diffusive dynamics in bilayers and monolayers, focusing on potential lipid-specific interactions between opposing leaflets. Through quantitative fluorescence microscopy techniques, we characterize lipid diffusion and mean molecular area in monolayers and bilayers composed of different lipids. Our results suggest that the observed decrease in bilayer lipid diffusion compared to monolayers depends on lipid identity. Furthermore, our analysis suggests that lipid acyl chain structure and spatial configuration at the bilayer may strongly influence interleaflet interactions and dynamics in bilayers. These findings provide insights into the role of lipid structure in mediating interleaflet coupling and underscore the need for further experimental investigations to elucidate the underlying mechanisms.

show abstract

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models

Cited by 9 publications

References 71 publications

Influenza A M2 recruits M1 to the plasma membrane: a fluorescence fluctuation microscopy study

Influenza A M2 recruits M1 to the plasma membrane: a fluorescence fluctuation microscopy study

Influenza A virus hemagglutinin prevents extensive membrane damage upon dehydration

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Contact Info

Product

Resources

About