Effect of erythropoietin on the glucose transport of rat erythrocytes and bone marrow cells

Ghosal, Jharna; Chakraborty, Munmun; Biswas, Tanmay; Ganguly, Chayan K.; Datta, Asoke G.

doi:10.1016/0885-4505(87)90072-7

Cited by 10 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effects were observed in vitro by incubating peripheral blood RBC with Epo as well as shortly (3 hrs) after injection of Epo into β -thalassemic mice. These results are in agreement with reports by Datta et al who showed that Epo has multiple effects on mature RBC, including an anti-oxidative one [37, 67–72]. …”

Section: Discussionsupporting

confidence: 94%

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

Amer

Dana

Fibach

2010

Anemia

View full text Add to dashboard Cite

Because of its stimulating effect on RBC production, erythropoietin (Epo) is used to treat anemia, for example, in patients on dialysis or on chemotherapy. In β-thalassemia, where Epo levels are low relative to the degree of anemia, Epo treatment improves the anemia state. Since RBC and platelets of these patients are under oxidative stress, which may be involved in anemia and thromboembolic complications, we investigated Epo as an antioxidant. Using flow-cytometry technology, we found that in vitro treatment with Epo of blood cells from these patients increased their glutathione content and reduced their reactive oxygen species, membrane lipid peroxides, and external phosphatidylserine. This resulted in reduced susceptibility of RBC to undergo hemolysis and phagocytosis. Injection of Epo into heterozygous (Hbbth3/+) β-thalassemic mice reduced the oxidative markers within 3 hours. Our results suggest that, in addition to stimulating RBC and fetal hemoglobin production, Epo might alleviate symptoms of hemolytic anemias as an antioxidant.

show abstract

Section: Discussionsupporting

confidence: 94%

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

Amer

Dana

Fibach

2010

Anemia

View full text Add to dashboard Cite

show abstract

“…However, specific binding of Epo to rat and human RBCs was reported (3,45). Epo was shown to have an effect on glucose transport (3,23), antioxidant defense systems (13,15), ion transport (5,45), and rheological properties (26,58) of mature RBCs. At present, no data are available on the Epo-binding sites on erythrocyte membranes or their distribution within RBC population.…”

mentioning

confidence: 99%

Erythropoietin activates nitric oxide synthase in murine erythrocytes

Mihov¹,

Vogel²,

Gassmann³

et al. 2009

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Erythropoietin (Epo) is the main regulator of erythrocyte production and a potent cytoprotective factor. It was suggested that some of Epo cytoprotective properties are due to its regulation of nitric oxide (NO) production. Recently, functionally active endothelial type NO synthase (eNOS) was discovered in mature murine and human red blood cells (RBC-eNOS). The goal of the present study was to characterize the effect of physiological and therapeutic doses of Epo on RBC-eNOS function. We found that recombinant human Epo (rHuEpo) binds specifically to mouse erythrocytes. Epo binding sites are not equally distributed through the RBC population but prevail in reticulocytes and young erythrocytes with about 105 receptors/cell, compared with adult and old erythrocytes containing 1-4 receptors/cell. The treatment of mouse erythrocytes with rHuEpo resulted in a time- and dose-dependent upregulation of NO production mediated via activation of the phosphatidylinositol-3-kinase /Akt pathway and RBC-eNOS phosphorylation at Ser-1177. Finally, when erythrocytes were incubated in L-arginine-free medium, rHuEpo treatment resulted in upregulation of superoxide radical production with concomitant shifting of the cellular redox state toward more oxidized state. Epo-induced changes in erythrocyte redox potential were absent in erythrocytes from eNOS-deficient mice.

show abstract

“…In contrast, Fraser et al [22] showed that OrthoEB from cultured human bone marrow cells retained 30% of the initial number of EPO-R. Working with cultured Friend virus infected mouse bone marrow cells Wickrema et al [26] -ATPase activity in rat and rabbit reticulocyte membranes [29,30] and human RBC [28]; activation of nitric oxide synthase activity in murine RTC and RBC [27]; glucose transport in rat RBC [31] reactive oxygen metabolism and in rat RBC [32] and the work of Lang et al [33] who have shown that EPO can inhibit eryptosis mediated in human RBC by a wide variety of insults (reviewed in Lang et al [33]). Thus, when levels of EPO-R agonist fall, the lack of signaling by the remaining EPO-R on RTC may result in their rapid clearance via eryptosis.…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Long-lived Erythropoietin Receptor Agonists in Rats

Bugelski¹,

Makropoulos²,

Doms³

2011

Pharm Anal Acta

View full text Add to dashboard Cite

Erythropoietin (EPO) regulates proliferation and differentiation of erythroid precursor cells into erythrocytes. Here, we report on experiments designed to study how the pharmacokinetic profiles of EPO receptor agonists, ranging from the short-lived epoetin-α to the long-lived EPO-MIMETIBODY TM constructs CNTO 530 and CNTO 531, influence the pharmacodynamic response in rats. Rats received a single dose of an EPO-R agonist and the effects on reticulocytes, red blood cells and hemoglobin were measured over time. The increase in red blood cells and hemoglobin were negatively correlated with clearance. At doses that cause a similar effect on reticulocytes, very long-lived EPO-R agonists caused prolonged production of red blood cells. In conclusion, we have shown that very long-lived EPO-R agonists cause prolonged production of red blood cells and increase in hemoglobin that is independent of their in vitro potency or the peak release of reticulocytes. These data suggest that EPO may be a survival factor for reticulocytes.

show abstract

Effect of erythropoietin on the glucose transport of rat erythrocytes and bone marrow cells

Cited by 10 publications

References 12 publications

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

Erythropoietin activates nitric oxide synthase in murine erythrocytes

Differential Effects of Long-lived Erythropoietin Receptor Agonists in Rats

Contact Info

Product

Resources

About