Effect of Escherichia coli and its soluble factors on mitochondrial membrane potential, phosphatidylserine translocation, viability, and motility of human spermatozoa

Schulz, Mabel; Sánchez, Raúl; Soto, L; Risopatrón, Jennie; Villegas, Jaime Eduardo Bernal

doi:10.1016/j.fertnstert.2009.01.140

Cited by 89 publications

(71 citation statements)

References 42 publications

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“…Furthermore, we have found a highly significant negative correlation between FSH and viable sperm with normal MMP, which confirms the findings by Ruwanpura et al [36], obtained in experimental conditions. Similarly, the changes in MMP already observed in cases of inflammation [9,35,40,47] were correlated with FSH, the main hormonal regulator of spermatogenesis; however, this relation did not exist with DNA denaturation, which is in agreement with the findings of Appasamy et al [1]. We have observed a highly significant correlation between dead, apoptotic and viable sperm, viable sperm with normal MMP, sperm with DNA denaturation and conventional sperm parameters, which is in agreement with the findings in the literature [3,6,27,41].…”

Section: Discussionmentioning

confidence: 68%

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

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Purpose To investigate the relationship between sperm apoptotic biomarkers and patient clinical characteristics, conventional sperm parameters and fertility potential. Material and methods Sperm analysis, phospholipid asymmetry, mitochondrial membrane potential (MMP) and DNA denaturation were assessed in 142 males of infertile couples. Seventy-three couples were allocated to the natural conception group, and 55 couples underwent IVF or ICSI. Results DNA denaturation correlated positively with age and negatively with testicular volume (TV). MMP correlated negatively with BMI and FSH and positively with TV. Normal viable sperm correlated positively with TV and negatively with age, BMI and FSH. DNA denaturation was associated with a significantly lower natural pregnancy rate (OR 5.4, p00.011). Conclusion Sperm apoptosis is related to male age, BMI, testicular volume and FSH. Among the apoptotic markers, only DNA denaturation has been found to predict natural pregnancy better than conventional sperm parameters.

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Section: Discussionmentioning

confidence: 68%

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

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“…Lastly, C. trachomatis infection of semen in vivo has been found to increase mitochondrial depolarization, as well as caspase-3 activation [15]. A significant reduction in ∆ym, viability, and motility was also observed after the incubation of sperm with E. coli and its soluble factors [7]. In another experimental study, the loss of sperm motility induced by the soluble products of E. coli was accompanied by the increased intrinsic mitochondrial generation of ROS and membrane lipid peroxidation [8].…”

Section: Apoptosis/necrosismentioning

confidence: 94%

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

Frączek

Kurpisz

2015

Folia Histochem Cytobiol.

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The invasion of the male reproductive tract by microorganisms, and its subsequent consequences for sperm fertilizing potential, has been intensely discussed. The role of the bacteria that are responsible for the colonization and contamination of the male urogenital tract, rather than its infection, in diminished sperm parameters raises the most controversy. There are numerous premises suggesting that bacterial semen infection is associated with male infertility. However, the molecular mechanism by which the fertility is affected is complex and multifactorial, and still presents a puzzle. Some authors have suggested that direct interactions between bacteria and human spermatozoa facilitate sperm immobilization, affect sperm morphology, and thus weaken the ability of sperm to fertilize. On the other hand, the massive infiltration of activated leukocytes into the inflammatory site may be associated with impairment of sperm fertilizing potential, due to oxidative, apoptotic, and immune processes. This review presents current research trends and aims to summarize the present knowledge of semen inflammation and causative bacterial agents in the male urogenital tract, with its consequence on seminological parameters, and male fertility status.

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“…They noticed that the early apoptosis incident (phosphatidyl serine [PS] externalization) was significantly increased in spermatozoa after incubation with E. coli alone. Other in vitro studies demonstrated that the soluble products of E. coli decreased sperm motility by causing defects in the sperm's mitochondrial function (108,109). SIF was isolated from E. coli by Prabha et al (110), where they reported that the incubation of spermatozoa with SIF causes sperm immobilization and structural modification.…”

Section: Escherichia Colimentioning

confidence: 99%

The Impact of Bacterial Infections on Human Spermatozoa

Zeyad¹,

Amor²,

Hammadeh³

2017

International Journal of Women’s Health and Reproduction Scienc

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Effect of Escherichia coli and its soluble factors on mitochondrial membrane potential, phosphatidylserine translocation, viability, and motility of human spermatozoa

Cited by 89 publications

References 42 publications

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

The Impact of Bacterial Infections on Human Spermatozoa

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