Effect of essential oils of Lamiaceae plants on the Rhizopus spp.

Abstract

The aim of this study was to evaluate the fungicidal effect of eleven essential oils against six isolates of the genus Rhizopus. Isolates were obtained from various moldy foods (chestnut, bread, strawberry, nectarine, blackberry and cherry tomatoes). The essential oils used in this study were extracts of basil (Oscimum basilicum L.), hyssop (Hyssopus officinalis L.), lavender (Lavandula angustifolia MILLER.), marjoram (Origanum majorana L.), mint (Mentha piperita L.), oregano (Origanum vulgare L.), rosemary (Rosmarinus officinalis L.), sage (Salvia officinalis L.), summer savory (Satureja hortensis L.), thyme (Thymus vulgaris L.) and wild thyme (Thymus serpyllum L.). Semi-quantitative composition of the essential oil samples was determined by gas chromatography coupled with mass spectrometry (GC-MS). The GC-MS analyses of the essential oils led to identification of 139 compounds, of which 49 were presented in ≥1% amount in at least one essential oil. The antifungal activity of essential oils against the Rhizopus spp. was determined, using microatmosphere method (0.625 μL.ml -1 of air), during 7 days. Seven essential oils: thyme, mint, summer savory, lavender, marjoram, oregano and wild thyme completely inhibited the growth of all isolates. Other essential oils have different effects on the growth of isolates. Basil essential oil stimulated growth of two isolates on the second day of cultivation. The growth of other isolates was, by contrast, inhibited by this essential oil in the same time of cultivation. Hyssop essential oil completely inhibited growth of two isolates, other 4 isolates were inhibited to fourth day of cultivation. In conclusion, certain essential oils are highly effective in vapour phase and can be used in another test of their antifungal activity and could be used in control of Rhizopus spp. or other fungal pathogens.

The Use of Essential Oils from Thyme, Sage and Peppermint against Colletotrichum acutatum

“…Our research data confirmed that the chemical composition of tested EO is in line with what is already have been described in the literature [ 16 , 17 , 19 , 20 , 26 , 29 , 30 , 31 , 32 , 33 , 34 ]. Oliveira et al [ 16 ] reported that thymol and p -cymene were abundant components in identified 28 compounds of thyme EO.…”

“…After reviewing the previous literature [ 6 , 24 , 32 ], the assumption could be that the higher content of menthol in the essential oil has a higher inhibitory effect against Colletotrichum spp. The antifungal properties of sage EO primarily affect the main abundant constituents α,β-thujone, camphor, and eucalyptol [ 20 , 29 , 33 , 34 ]. In our research, the antifungal activity of sage EO against C. acutatum achieved the highest effect of 88.14% at 1800 μL L −1 .…”

Antioxidant, Antimicrobial and Antibiofilm Activity of Coriander (Coriandrum sativum L.) Essential Oil for Its Application in Foods

“…The gas chromatography coupled with mass spectrometry (GC-MS, Agilent 7890B, Agilent 5977A, Agilent Technologies Inc., Palo Alto, CA, USA) and CombiPal autosampler 120 (CTC Analytics AG, Zwingen, Switzerland) was used for semi-quantitative composition of the coriander essential oil with modifications [18]. The column DB-WAXms ® with polyethylene glycol stationary phase (30 m × 0.25 mm × 0.25 µm) (Agilent Technologies, Santa Clara, CA, USA) was used.…”

Introduction of hyssopus officinalis l . into in vitro culture to optimize the conditions for obtaining callus tissues and microclonal propagation as a promising metod of innovative agrobiotechnologies

“…In experiments, conducted by scientists, Hyssopus officinalis exhibits antibacterial, antimycotic and antiprotozoal activity [19], and also has fungicidal properties [20].…”