Effect of estradiol during culture of bovine oocyte–granulosa cell complexes on the mitochondrial DNA copies of oocytes and telomere length of granulosa cells

Endo, M; Kimura, Koji; Kuwayama, Takehito; Monji, Yasunori; Iwata, Hisataka

doi:10.1017/s0967199412000603

Cited by 18 publications

(18 citation statements)

References 31 publications

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“…Thus, it is plausible that mtDNA number is controlled by both biosynthesis and degradation. In addition, our recent study shows that mtDNA number in oocytes increases during in vitro oocyte growth from the early antral follicle stage to the antral follicle stage and that the increase ratio varies to a great extent and depends on the individual donors [30]. In agreement with this result, in the present study, the increase ratio for mtDNA number also varied ranging from 0.15 to 6.26.…”

Section: Discussionsupporting

confidence: 89%

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Takeo

Goto

Kuwayama

et al. 2013

Journal of Reproduction and Development

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Age-associated deterioration in both the quality and quantity of mitochondria occurs in older women. The main aim of this study was to examine the effect of age on mitochondrial DNA copy number (mtDNA number) in early developmental stage bovine embryos as well as the dynamics of mtDNA number during early embryo development. Real-time PCR was used to determine mtDNA number. In vitro-produced embryos 48 h after insemination derived from Japanese black cows, ranging in age from 25 to 209 months were categorized based on their cleavage status. There was an overall negative relationship between the age of the cow and cleavage status, to the extent that the ratio of embryos cleaved over the 4-cell stage was greater in younger cows. The mtDNA number did not differ among the cleaved status of embryos. In the next experiment, oocytes collected from each donor cow were divided into 2 groups containing 10 oocytes each, in order to compare the mtDNA number of mature oocytes and early developmental stage embryos within individuals. Upon comparing the mtDNA number between oocytes at the M2 stage and early developmental stage 48 h post insemination, mtDNA number was found to decrease in most cows, but was found to increase in some cows. In conclusion, age affects the cleaving ability of oocytes, and very old cows (> 180 months) tend to have lower mtDNA numbers in their oocytes. The change in mtDNA number during early development varied among individual cows, although overall, it showed a tendency to decrease.

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Section: Discussionsupporting

confidence: 89%

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Takeo

Goto

Kuwayama

et al. 2013

Journal of Reproduction and Development

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“…Based on these results, we concluded that the stimulatory effect of Res on mitochondrial biosynthesis depends on the individual conditions of donor cows and that there may be additional unknown factors in oocytes contributing to mitochondrial biosynthesis. In agreement with these results, when oocytes of early antral follicles were cultured and the kinetics of Mt numbers in oocyte were examined, the ration of increase in Mt number in oocytes varied substantially among donor cows [29]. We observed a similar trend in our results for MMP.…”

Section: Discussionsupporting

confidence: 88%

Resveratrol Improves the Mitochondrial Function and Fertilization Outcome of Bovine Oocytes

et al. 2014

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The aim of the present study was to address the effect of resveratrol-mediated upregulation of sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, the developmental ability of oocytes and on mechanisms responsible for blockage of polyspermic fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in TCM-199 medium supplemented with 10% FCS and 0 or 20 µM resveratrol (Res). We examined the effect of Res on SIRT1 expression in in vitro-matured oocytes (Exp 1); fertilization and developmental ability (Exp 2); mitochondrial DNA copy number (Mt number), ATP content and mitochondrial membrane potential in matured oocytes (Exp 3); and the time required for proteinase to dissolve the zona pellucida following in vitro fertilization (as a marker of zona pellucida hardening), as well as on the distribution of cortical granules before and after fertilization (Exp 4). In Exp 1, the 20 µM Res treatment upregulated protein expression of SIRT1 in oocytes. In Exp 2, Res treatment improved the ratio of normal fertilization and the total cell number of blastocysts. In Exp 3, Res treatment significantly increased the ATP content in matured oocytes. Additionally, Res increased the overall Mt number and mitochondrial membrane potential, but the effect was donor-dependent. In Exp 4, Res-induced zona hardening improved the distribution and exocytosis of cortical granules after in vitro fertilization. In conclusion, Res improved the quality of oocytes by improving mitochondrial quantity and quality. In addition, Res added to the maturation medium enhanced SIRT1 protein expression in oocytes and improved fertilization via reinforcement of the mechanisms responsible for blockage of polyspermic fertilization.

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“…Mitochondrial number can be predicted by quantifying the mitochondrial DNA copy number (Mt number) using real time-PCR, based on reports that mitochondria in oocytes typically contain one copy of the mitochondrial genome [4]. The Mt-number in bovine oocytes increases during the growth of oocytes from early antral follicles to antral follicles in vivo and in vitro [5], [6], and the number remains the same or increases further during oocyte maturation [5], [7], [8]. However, little is known about how culture conditions affect Mt number in oocytes.…”

Section: Introductionmentioning

confidence: 99%

Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes

et al. 2014

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The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 µM resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the ability of oocytes to develop into the blastocyst stage following activation. The effects of resveratrol on mitochondrial number were examined by comparing the mitochondrial DNA copy number (Mt number) between group of oocytes collected from the same donor gilt ovaries. Supplementing the maturation medium with only resveratrol did not affect the Mt number in the oocytes. However, supplementing the maturation medium with 10 µM MG132, a proteasome inhibitor, significantly increased the amount of ubiquitinated proteins and Mt number by 12 and 14%, respectively. In addition, when resveratrol was added to the medium containing MG132, the Mt number increased significantly by 39%, this effect was diminished by the addition of the SIRT1 inhibitor EX527. Furthermore, supplementing the medium with MG132 and EX527 did not affect Mt number. The mean SIRT1 expression in 20 oocytes was significantly and positively correlated with the Mt number in oocytes collected from the same donor. This study suggests that the expression of SIRT1 is associated with the Mt number in oocytes. In addition, activation of SIRT1 by resveratrol enhances the biosynthesis and degradation of mitochondria in oocytes, thereby replenishing and improving mitochondrial function and the developmental ability of oocytes.

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Effect of estradiol during culture of bovine oocyte–granulosa cell complexes on the mitochondrial DNA copies of oocytes and telomere length of granulosa cells

Cited by 18 publications

References 31 publications

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Resveratrol Improves the Mitochondrial Function and Fertilization Outcome of Bovine Oocytes

Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes

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