Effect of estrogen on angiogenesis in co-cultures of human endometrial cells and microvascular endothelial cells

Albrecht, Eugene D.; Babischkin, Jeffery S.; Lidor, Y.; Anderson, Larry D.; Udoff, Lawrence C.; Pepe, Gerald J.

doi:10.1093/humrep/deg415

Cited by 82 publications

(59 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A further increase was observed when endothelial cells were co-cultured with epithelial cells and oestrogen. Tube formation was similar to that in media only when endothelial cells were co-cultured with endometrial stromal cells, with and without oestrogen [82]. This research suggests that oestrogen regulates angiogenesis by controlling the expression and secretion of angiogenic factors such as VEGF by endometrial epithelial cells.…”

Section: Role Of Vegf In Endometrial Angiogenesissupporting

confidence: 54%

“…This suggests that epithelial VEGF is unlikely to have a role in endometrial angiogenesis [81]. However, research by Albrecht et al [82], who established methodology to co-culture endometrial epithelial and stromal cells with human myometrial microvascular endothelial cells, does not support this hypothesis. Human recombinant VEGF increased myometrial microvascular endothelial cell tube formation by approximately 65% over formation in media only.…”

Section: Role Of Vegf In Endometrial Angiogenesismentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in endometrial angiogenesis research

2005

View full text Add to dashboard Cite

This review summarises recent research into the mechanisms and regulation of endometrial angiogenesis. Understanding of when and by what mechanisms angiogenesis occurs during the menstrual cycle is limited, as is knowledge of how it is regulated. Significant endometrial endothelial cell proliferation occurs at all stages of the menstrual cycle in humans, unlike most animal models where a more precise spatial relationship exists between endothelial cell proliferation and circulating levels of oestrogen and progesterone. Recent stereological data has identified vessel elongation as a major endometrial angiogenic mechanism in the mid-late proliferative phase of the cycle. In contrast, the mechanisms that contribute to post-menstrual repair and secretory phase remodelling have not yet been determined. Both oestrogen and progesterone/progestins appear to have paradoxical actions, with recent studies showing that under different circumstances both can promote as well as inhibit endometrial angiogenesis. The relative contribution of direct versus indirect effects of these hormones on the vasculature may help to explain their pro- or anti-angiogenic activities. Recent work has also identified the hormone relaxin as a player in the regulation of endometrial angiogenesis. While vascular endothelial growth factor (VEGF) is fundamental to endometrial angiogenesis, details of how and when different endometrial cell types produce VEGF, and how production and activity is controlled by oestrogen and progesterone, remains to be elucidated. Evidence is emerging that the different splice variants of VEGF play a major role in regulating endometrial angiogenesis at a local level. Intravascular neutrophils containing VEGF have been identified as having a role in stimulating endometrial angiogenesis, although other currently unidentified mechanisms must also exist. Future studies to clarify how endometrial angiogenesis is regulated in the human, as well as in relevant animal models, will be important for a better understanding of diseases such as breakthrough bleeding, menorrhagia, endometriosis and endometrial cancer.

show abstract

Section: Role Of Vegf In Endometrial Angiogenesissupporting

confidence: 54%

Section: Role Of Vegf In Endometrial Angiogenesismentioning

confidence: 99%

Recent advances in endometrial angiogenesis research

2005

View full text Add to dashboard Cite

show abstract

“…In addition, it is known that both E 2 and PGE 2 induce the expression of vascular endothelial growth factor (VEGF) and, in turn, VEGF induces the expression of COX-2 (11)(12)(13). Vascular endothelial growth factor is one of the most important angiogenic factors involved in the pathogenesis of endometriosis (14,15).…”

mentioning

confidence: 99%

Effect of aromatase inhibitors on ectopic endometrial growth and peritoneal environment in a mouse model of endometriosis

Bilotas

Meresman

Stella³

et al. 2010

Fertility and Sterility

View full text Add to dashboard Cite

“…VEGF also increases endometrial endothelial cell tubulogenesis and transcriptome changes which are associated with angiogenesis. The production and secretion of VEGF in the endometrium are largely attributed to the glandular epithelial cells, which produce increased amounts of VEGF with increased estrogen stimulation [ 59 ]. VEGF then exerts its proangiogenic effects by binding the VEGF receptors present on endometrial endothelial cells, causing increased proliferation and blood vessel growth in the endometrium.…”

Section: Estrogen and Menstruationmentioning

confidence: 99%

Role of Sex Steroids in Angiogenesis

Lecce

Lam

2013

Biochemical Basis and Therapeutic Implications of Angiogenesis

View full text Add to dashboard Cite

Sex steroids such as estrogen and testosterone are key mediators of angiogenesis. They are implicated in both physiological and pathological angiogenesis such as during the menstrual cycle, wound healing, and cancer growth and progression. Sex steroids regulate many aspects of angiogenesis through both classic genomic signaling pathways which regulate gene expression and also rapid action nongenomic pathways. In this capacity, sex steroids are able to modulate endothelial and progenitor cell functions such as proliferation, migration, and attachment, which are all essential components involved in neovascularization. Since sex steroids are known to augment angiogenesis which is vital to tumor progression and growth, common treatment of hormone-responsive tumors is through sex steroid receptor antagonists. Due to the involvement of sex steroids in necessary physiological functions as well as the potential to promote pathological angiogenesis, it is fundamental that the mechanisms behind sex steroid-mediated neovascularization are understood.

show abstract

Effect of estrogen on angiogenesis in co-cultures of human endometrial cells and microvascular endothelial cells

Abstract: We propose that estrogen, by regulating expression and secretion of angiogenic factors such as VEGF by glandular epithelial cells of the endometrium, regulates endometrial angiogenesis.

Cited by 82 publications

References 31 publications

Recent advances in endometrial angiogenesis research

Recent advances in endometrial angiogenesis research

Effect of aromatase inhibitors on ectopic endometrial growth and peritoneal environment in a mouse model of endometriosis

Role of Sex Steroids in Angiogenesis

Contact Info

Product

Resources

About