Effect of estrogen on the expression of occludin in ovariectomized mouse brain

Kang, Han Seung; Ahn, Hae Sun; Kang, Hee Jeong; Gye, Myung Chan

doi:10.1016/j.neulet.2006.03.052

Cited by 35 publications

(23 citation statements)

References 32 publications

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“…However, this does not preclude the possibility that estrogen may regulate other tight junction family members such as the junction associated molecules (JAMs). Estrogen has been shown to increase occludin mRNA in whole brain homogenates (Kang et al, 2006) and in the colon and a human colon cell line (Braniste et al, 2009). However, estrogen also increases permeability of endometrial tight junctions (Aberdeen et al, 2008) and alters occludin levels in a biphasic manner in human umbilical cord derived endothelial cells (Wolburg et al, 2003) indicating that the hormone may have pleiotrophic effects.…”

Section: Discussionmentioning

confidence: 99%

Reproductive age-related changes in the blood brain barrier: Expression of IgG and tight junction proteins

Bake

Friedman

Sohrabji

2009

Microvascular Research

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We previously demonstrated that there is significantly greater transfer of intravenously-injected Evan’s blue dye into the forebrain of acyclic (reproductive senescent) females compared to young adult females, indicating that blood brain barrier permeability is compromised in the reproductive senescent forebrain. The present study examined brain IgG expression and microvessel tight junction proteins to assess ovarian age-related changes in microvascular permeability, and further compared young and senescent females with age-matched males to distinguish changes attributable to age and reproductive senescence. Blood brain barrier breakdown are often associated with increased extravasation of plasma proteins and high levels of immunoglobulin G (IgG) in brain. In the present study, IgG expression was dramatically increased in the hippocampus and thalamus, but not the hypothalamus of reproductive senescent females compared to young adult females. In males, IgG expression was increased in all these regions in middle aged animals (aged-matched to senescent females) as compared to young males (age-matched to the young adult females). Furthermore, the proportion of hippocampal microvessels with perivascular IgG immunoreactivity was significantly greater in reproductive senescent females as compared to young adult females, while middle aged males and young adult males did not differ. The tight junctions between adjacent microvascular endothelial cells regulated by transmembrane proteins such as claudin-5 and occludin play a critical role in maintaining the blood brain barrier integrity. Increased hippocampal IgG expression in senescent females was paralleled by poor junctional localization of the tight junction protein claudin-5 in hippocampal microvessels. However, there was no difference in hippocampal claudin-5 localization between young adult and middle aged males, indicating that dysregulation of this junctional protein was associated with ovarian aging. Parallel studies in human brain microvessels also revealed age-dependent disruption in claudin-5 distribution in post-menopausal women compared to premenopausal women. Collectively, these data support the hypothesis that constitutive loss of barrier integrity in the forebrain during reproductive senescence may be due, in part, to the selective loss of tight junction proteins in endothelial junctions.

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Section: Discussionmentioning

confidence: 99%

Reproductive age-related changes in the blood brain barrier: Expression of IgG and tight junction proteins

Bake

Friedman

Sohrabji

2009

Microvascular Research

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“…For example, the mitogenic effects of estrogens on hormone-dependent breast and ovarian cancer cells are, at least in part, the consequence of an increased expression of genes involved in cell cycle regulation [12]. In hormone-dependent cells, E2 is also known to activate expression of genes directly or indirectly associated with a differentiated cellular phenotype [13,14].…”

Section: Introductionmentioning

confidence: 99%

Expression of differentiation-associated gene icb-1 is estrogen-responsive in ovarian and breast cancer cell lines

Bollmann

Ortmann

Treeck

2008

The Journal of Steroid Biochemistry and Molecular Biology

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“…Its formation and function is regulated by several key factors like VEGF and Wnt (Tam and Watts, 2010;Liebner et al, 2011), and is influenced by hormones as well (Cucullo et al, 2004;Kang et al, 2006;Muresanu et al, 2010;Sadowska et al, 2010;Rosas-Hernandez et al, 2013a;Salvador et al, 2014). Here we show for the first time that inhibition of PRL with Bromo increases the permeability the BBB in vivo through the expression of the TJ proteins claudin-5 and occludin.…”

Section: Discussionmentioning

confidence: 60%

“…These molecules are key factors in the development and acquisition of the BBB phenotype (Liebner et al, 2008;Tam and Watts, 2010). In addition, it has been reported that glucocorticoids (Sadowska et al, 2010;Salvador et al, 2014) and hormones like estrogens (Kang et al, 2006), growth hormone (GH) (Muresanu et al, 2010) and prolactin (PRL) (Rosas-Hernandez et al, 2013a,b) can modulate different features of the BBB both in vitro (Cucullo et al, 2004;Rosas-Hernandez et al, 2013a,b) and in vivo (Kang et al, 2006;Muresanu et al, 2010). These last two hormones are members of the PRL family.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of prolactin with bromocriptine for 28days increases blood–brain barrier permeability in the rat

Rosas-Hernández¹,

Ramı́rez²,

Ramirez-Lee³

et al. 2015

Neuroscience

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Effect of estrogen on the expression of occludin in ovariectomized mouse brain

Cited by 35 publications

References 32 publications

Reproductive age-related changes in the blood brain barrier: Expression of IgG and tight junction proteins

Reproductive age-related changes in the blood brain barrier: Expression of IgG and tight junction proteins

Expression of differentiation-associated gene icb-1 is estrogen-responsive in ovarian and breast cancer cell lines

Inhibition of prolactin with bromocriptine for 28days increases blood–brain barrier permeability in the rat

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