Effect of estrogen replacement on the histologic response to polypropylene mesh implanted in the rabbit vagina model

Higgins, Edmund; Rao, Arundhati; Baumann, Shannon S.; James, Rebecca; Kuehl, Thomas J.; Muir, Tristi W.; Pierce, Lisa

doi:10.1016/j.ajog.2009.05.050

Cited by 26 publications

(22 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together, these findings suggest that vaginal atrophy induced by decreases in ovarian hormones includes a decrease in the vaginal muscularis that is proportional to decreases in other layers of the vaginal wall resulting in little change of the calculated percentage of the wall composed of smooth muscle. Our results are in support of previous studies of the rabbit 4 and 12 weeks following ovariectomy [] and 4 week ovariectomized rodents []. Our results are also in support of findings obtained with human tissue as it has been shown that MHC mRNA transcript expression was not altered within the vaginal muscularis of postmenopausal compared with premenopausal women .…”

Section: Discussionsupporting

confidence: 93%

Effect of Estrogen on Molecular and Functional Characteristics of the Rodent Vaginal Muscularis

Basha

Chang

Burrows

et al. 2013

The Journal of Sexual Medicine

View full text Add to dashboard Cite

Introduction Vaginal atrophy is a consequence of menopause however little is known concerning the effect of a decrease in systemic estrogen on vaginal smooth muscle structure and function. As the incidence of pelvic floor disorders increases with age, it is important to determine if estrogen regulates the molecular composition and contractility of the vaginal muscularis. Aim The goal of this study was to determine the effect of estrogen on molecular and functional characteristics of the vaginal muscularis utilizing a rodent model of surgical menopause. Methods 3–4 month old Sprague Dawley rats underwent sham laparotomy (Sham, n=18) or ovariectomy (Ovx, n=39). Two weeks following surgery, animals received a subcutaneous osmotic pump containing vehicle (Sham, Ovx) or 17- β estradiol (Ovx). Animals were euthanized one week later and the proximal vagina was collected for analysis of contractile protein expression and in vitro studies of contractility. Measurements were analyzed using a one-way ANOVA followed by Tukey's post hoc analysis (α= 0.05). Main Outcome Measures Protein and mRNA transcript expression levels of contractile proteins, in vitro measurements of vaginal contractility Results Ovariectomy decreased the expression of carboxyl-terminal myosin heavy chain isoform SM1 and h-caldesmon and reduced the amplitude of contraction of the vaginal muscularis in response to KCl. Estradiol replacement reversed these changes. No differences were detected in the % vaginal muscularis, mRNA transcript expression of amino terminal MHC isoforms, l-caldesmon expression and maximal velocity of shortening. Conclusion Systemic estrogen replacement restores functional and molecular characteristics of the vaginal muscularis of ovariectomized rats. Our results indicate that menopause is associated with changes in the vaginal muscularis, which may contribute to the increased incidence of pelvic floor disorders with age.

show abstract

Section: Discussionsupporting

confidence: 93%

Effect of Estrogen on Molecular and Functional Characteristics of the Rodent Vaginal Muscularis

Basha

Chang

Burrows

et al. 2013

The Journal of Sexual Medicine

View full text Add to dashboard Cite

show abstract

“…In humans, menopause was found to be associated with decreased amounts of type I collagen in the female pelvis (17). A recent study (18) reported on decreased collagen deposition in synthetic polypropylene mesh implanted in the vagina of oophorectomized rabbits. This phenomenon was reversed by E replacement therapy.…”

Section: Abramov Correspondence Fertil Steril 2011mentioning

confidence: 99%

Effect of bilateral oophorectomy on wound healing of the rabbit vagina

Abramov¹,

Webb²,

Botros³

et al. 2011

Fertility and Sterility

View full text Add to dashboard Cite

“…In rabbits, successful insertion of implants in the posterior vagina has been described, but grafted material is by definition small [27][28][29][30][31][32][33]. In contrast to abdominal repairs, rabbit vaginal repairs with mesh result in exposure rates as high as 50%, which is far above that reported clinically [30,31,34,35]. It is, therefore, uncertain whether the mechanism that is driving extrusion in this model is similar to what is happening clinically.…”

Section: Purpose and Design Of Animal Models And Experimentsmentioning

confidence: 85%

A standardized description of graft-containing meshes and recommended steps before the introduction of medical devices for prolapse surgery

et al. 2012

View full text Add to dashboard Cite

Over the past decade, a huge number of new implants and ancillary devices have been introduced to the market. Most of these have become clinically available with little or no clinical data or research. This is a less-than-ideal situation, and this subgroup of the ad hoc IUGA roundtable conference wants to open the discussion to change this, by proposing a pragmatic minimum clearance track for new products being introduced to the market. It consists of an accurate and more standardized product description, data on the biological properties gathered in animal experiments, anatomical cadaveric studies, and upfront clinical studies followed by a compulsory registry on the first 1,000 patients implanted. Ideally, manufacturers should support well-designed prospective (randomized) clinical trials that can support the claimed benefits of the new product.

show abstract

Effect of estrogen replacement on the histologic response to polypropylene mesh implanted in the rabbit vagina model

Cited by 26 publications

References 21 publications

Effect of Estrogen on Molecular and Functional Characteristics of the Rodent Vaginal Muscularis

Effect of Estrogen on Molecular and Functional Characteristics of the Rodent Vaginal Muscularis

Effect of bilateral oophorectomy on wound healing of the rabbit vagina

A standardized description of graft-containing meshes and recommended steps before the introduction of medical devices for prolapse surgery

Contact Info

Product

Resources

About