Effect of Ethanol Extract of Trigonella foenum graecum (Fenugreek) Seeds on Freund's Adjuvant-Induced Arthritis in Albino Rats

Suresh, P.; Kavitha, Ch. N.; Babu, Suresh; Reddy, V. Prabhakar; Latha, A. Kanaka

doi:10.1007/s10753-012-9444-7

Cited by 79 publications

(67 citation statements)

References 27 publications

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“…Diclofenac was chosen as a reference anti-inflammatory agent due to its proven anti-arthritic effects in Adjuvant-induced arthritis [27, 28]. Its selected dose is in accordance with previous literature [27, 28]. The chosen regimen is in accordance with previous reports [23, 29].…”

Section: Methodsmentioning

confidence: 99%

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Arab

Salama²,

Abdelghany

et al. 2017

Cell Physiol Biochem

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Background/Aims: Camel milk (CM) has shown beneficial anti-inflammatory actions in several experimental and clinical settings. So far, its effect on rheumatoid arthritis (RA) has not been previously explored. Thus, the current work aimed to evaluate the effects of CM in Adjuvant-induced arthritis and air pouch edema models in rats, which mimic human RA. Methods: CM was administered at 10 ml/kg orally for 3 weeks starting on the day of Freund’s adjuvant paw inoculation. The levels of TNF-α and IL-10 were measured by ELISA while the protein expression of NF-κBp65, COX-2 and iNOS was detected by immunohistochemistry. The expression of MAPK target proteins was assessed by Western blotting. Results: CM attenuated paw edema, arthritic index and gait score along with dorsal pouch inflammatory cell migration. CM lowered the TNF-α and augmented the anti-inflammatory IL-10 levels in sera and exudates of arthritic rats. It also attenuated the expression of activated NF-κBp65, COX-2 and iNOS in the lining of the dorsal pouch. Notably, CM inhibited the MAPK pathway signal transduction via lowering the phosphorylation of p38 MAPK, ERK1/2 and JNK1/2 in rat hind paws. Additionally, CM administration lowered the lipid peroxide and nitric oxide levels and boosted glutathione and total anti-oxidant capacity in sera and exudates of animals. Conclusion: The observed CM downregulation of the arthritic process may support the interest of CM consumption as an adjunct approach for the management of RA.

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Section: Methodsmentioning

confidence: 99%

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Arab

Salama²,

Abdelghany

et al. 2017

Cell Physiol Biochem

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“…Literature frequently reported anti-inflammatory effects of fenugreek seed extracts (13, 25, 26). We thus tested the hypothesis that Stifenia could decrease the LPS-induced IL-1β production in PBMC.…”

Section: Resultsmentioning

confidence: 99%

Inflammatory Effects of the Plant Protection Product Stifenia (FEN560) on Vertebrates

Teyssier

Colussi²,

Delemasure³

et al. 2017

Front. Public Health

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Plant defense stimulators (PDSs) rely on the activation of plant innate immunity in order to protect crops against various pests. These molecules are thought to be a safer alternative to classical plant protection products. Given that innate immune systems share common features in plants and vertebrates, PDS can potentially cross-react with innate immunity of non-target organisms. To test this hypothesis, we studied effects of the commercial PDS Stifenia (FEN560), which is composed of crushed fenugreek seeds. We tested various concentrations of Stifenia (0.03–1 mg mL−1) on human peripheral blood mononuclear cells and checked, 20 h later, cell metabolic activity (MA) using XTT assay, cell death by flow cytometry analysis, and IL-1β inflammatory cytokine released in the culture medium using ELISA. Stifenia induced a general decrease of the cell MA, which was concomitant with a dose-dependent release of IL-1β. Our results highlight the activation of human immune cells. The inflammatory effect of Stifenia was partially inhibited by pan-caspase inhibitor. Accordingly, Stifenia induced the release of p20 caspase-1 fragment into the culture medium suggesting the involvement of the NLRP3 inflammasome. Furthermore, we observed that Stifenia can induce cell death. We also tested the effect of Stifenia on Zebrafish larvae. After 24 h of exposure, Stifenia induced a dose-dependent IL-1β and TNFα gene expression. The human-cell-based approach developed in this work revealed a high sensitivity concerning inflammatory properties of a plant protection product. These tests could be routinely used to screen the potential adverse effects of this type of compounds. Finally, our results suggest a potential danger of using extensively certain PDS for crop protection.

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“…In addition, the body weight was regarded as an indirect index to evaluate the development of adjuvant-induced arthritis (Calvino et al, 1987;Chitme and Patel, 2009). The previous papers reported that a loss of body weight was observed during the arthritic process in an adjuvant-induced arthritis rat model (Walz et al, 1971;Suresh et al, 2012). The loss of body weight is due to the increasing severity of arthritis.…”

Section: Discussionmentioning

confidence: 96%

Effects of Cichoric Acid Extract fromEchinacea purpureaon Collagen-Induced Arthritis in Rats

Jiang

Wang

et al. 2014

Am. J. Chin. Med.

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Cichoric acid extract (CAE) from Echinacea purpurea L. was used to investigate the anti-arthritic effect by using collagen-induced arthritis (CIA) rat model. The hind paw swelling volume and the body weight were measured and recorded. All the drug solutions were administered orally to rats for a total of 28 days. On day 28, the rats were anaesthetized and decapitated. The thymus and spleen were weighed for the determination of the organ index. The concentration of tumor necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and prostaglandin E2 (PGE-2) in the serum was measured using commercially available ELISA kits. Total and phosphor-NF-κB and Cox-2 protein expression in synovial tissues were determined by histological slides quantification and western blot analysis. Our data showed that administration of all doses of CAE (8, 16, and 32 mg/kg) significantly decreased the paw swelling, restored body weight gain and decreased the organ index of the thymus and spleen compared with that of the CIA group. CAE (8, 16, and 32 mg/kg) treatment significantly reduced the levels of TNFα, IL-1β and PGE-2 in serum compared with the CIA group. Histopathological analysis demonstrated that CAE has obvious anti-arthritic activity. In addition, CAE (32 mg/kg) significantly decreased the levels of nuclear factor-κB (NF-κB), TNFα and cyclooxygenase 2 (Cox-2) in synovium tissues of the ankle joint compared with the CIA group. Furthermore, CAE administration significantly decreased the protein expression of phosphor-NF-κB and Cox-2 in synovium tissues of the knee joint compared with the CIA group. The results suggest that the anti-inflammatory activity of CAE may account for its anti-arthritic effect, and CAE could be a potential therapeutic drug for the treatment of rheumatoid arthritis (RA).

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Effect of Ethanol Extract of Trigonella foenum graecum (Fenugreek) Seeds on Freund's Adjuvant-Induced Arthritis in Albino Rats

Cited by 79 publications

References 27 publications

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway

Inflammatory Effects of the Plant Protection Product Stifenia (FEN560) on Vertebrates

Effects of Cichoric Acid Extract fromEchinacea purpureaon Collagen-Induced Arthritis in Rats

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