Effect of ethanol on bacterial cellulose production from fructose in continuous culture

Naritomi, Takaaki; Kouda, Tohru; Yano, Hisato; Yoshinaga, Fumihiro

doi:10.1016/s0922-338x(98)80012-3

Cited by 91 publications

(71 citation statements)

References 18 publications

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“…Only a few reports evaluated the combined effect that the selection of carbon and nitrogen sources has on BC production by A. xylinum, although Ramana et al (2000) proposed different combinations that efficiently produce BC. Naritomi et al (1998) observed that the supplementation of 1% ethanol to a medium containing 30 g/L of fructose increases BC production by G. sucrofermentans BPR3001A. This study also suggests that ethanol acts not as a substrate for BC biosynthesis but as an energy source for ATP generation in the viable cells, increasing BC production as a result.…”

Section: Introductionmentioning

confidence: 60%

“…In the latter report, the authors suggest that ethanol was not the carbon source for BC production and that the improvement was a consequence of ethanol suppressing the transformation of glucose in gluconic acids, a process that potentiates a more efficient polymerization of glucose into BC. However, if the ethanol percentage is increased over 1.5%, BC production decreases due to the inhibition of cell growth caused by the accumulation of acetate (Naritomi et al 1998;Park et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

Santos¹,

Carbajo²,

Villar³

2013

BioResources

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Bacterial cellulose (BC) synthesized by Gluconacetobacter sucrofermentans has a high degree of crystallinity, durability, great resistance, and biocompatibility. This study evaluates the effect that carbon and nitrogen sources, present in HS (Hestrin-Schramm) culture medium, have on BC from G. sucrofermentans CECT 7291 used to restore damaged documents. The initial pH of the medium and the temperature were set for all assays, which were conducted in static conditions. The cellulose layers were obtained at four time points. The growth media were characterized at each time point (pH and carbon source consumption). Cellulose layers were washed, pressed, dried, and characterized by determining their pH, dry weight, and optical and mechanical properties. The best combination of carbon and nitrogen sources proved to be fructose plus yeast extract-corn steep liquor, with or without ethanol, which provided a good balance between BC production and carbon source consumption, and generated a resistant and homogeneous cellulose layer.

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Section: Introductionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

Santos¹,

Carbajo²,

Villar³

2013

BioResources

View full text Add to dashboard Cite

show abstract

“…In the present study, the effect of ethanol was not favourable. Ethanol supplementation has been reported to improve the quantity of cellulose in fructose (Naritomi et al 1998) or glucose media (Park et al 2003a). However, the presence of ethanol has also been related to a reduction in the growth of the cells that caused an initial delay in cellulose production that was corrected or even increased after the fifth day when compared with the media without ethanol (Park et al 2003a).…”

Section: Discussionmentioning

confidence: 99%

“…The growth conditions can induce changes in the cellulose production rate (Chawla et al 2009). The effects of nitrogen and phosphorus sources (Gomes et al 2013;Ramana et al 2000), pH (Verschuren et al 2000) and ethanol concentrations (Naritomi et al 1998;Park et al 2003a) on cellulose production by AAB have been evaluated. However, one of the most studied factors has been the influence of the carbon source, and different monosaccharides, disaccharides, and even alcohols have been tested (Keshk and Sameshima 2005;Mikkelsen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Cellulose production and cellulose synthase gene detection in acetic acid bacteria

Valera

Torija

Mas

et al. 2014

Appl Microbiol Biotechnol

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The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration by the traditional method. The vinegar biofilm produced by AAB on the air-liquid interface is primarily composed of cellulose and maintains the cells in close contact with oxygen. In this study, we screened for the ability of AAB to produce cellulose using different carbon sources in the presence or absence of ethanol. The presence of cellulose in biofilms was confirmed using the fluorochrome Calcofluor by microscopy. Moreover, the process of biofilm formation was monitored under epifluorescence microscopy using the Live/Dead BacLight Kit. A total of 77 AAB strains belonging to 35 species of Acetobacter, Komagataeibacter, Gluconacetobacter, and Gluconobacter were analysed, and 30 strains were able to produce a cellulose biofilm in at least one condition. This cellulose production was correlated with the PCR amplification of the bcsA gene that encodes cellulose synthase. A total of eight degenerated primers were designed, resulting in one primer pair that was able to detect the presence of this gene in 27 AAB strains, 26 of which formed cellulose.

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“…The strain produced a much smaller amount of BC with only glucose in medium than those that contain fructose or sucrose (data not shown). Though ethanol content in Plackett-Burman experimental design (X 5 in Table 1) had an influence on the crystallinity index of BC, it was also related to the carbon source for being considered to improve BC production as a substitute for glucose [23] or as an energy source for ATP generation [14]. By analyzing Fig.…”

Section: Discussionmentioning

confidence: 99%

Screening of the common culture conditions affecting crystallinity of bacterial cellulose

Zeng¹,

Liu²,

Chen³

et al. 2011

J Ind Microbiol Biotechnol

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By analyzing with X-ray diffraction and FT-IR spectroscopy, the main effective factors among common culture conditions on crystallinity index and I(α) fraction of cellulose produced by Gluconacetobacter xylinus were examined with Plackett-Burman design experiment. Varying pH value in the medium by adjusting the composition of citrate buffer or by adding HCl/NaOH solution indicates it is the content of citrate buffer rather than its function of pH buffering that gives the influence on crystallinity. Further experiment reveals that Na+ concentration of 0.174 mol/l in medium with citrate buffer added would decrease the crystallinity index significantly. Comparison of carbon sources shows that fructose leads to a higher crystallinity index than glucose, which suggests a relationship between crystallinity and production speed of bacterial cellulose affected by carbon sources. An interesting phenomenon was that a longer period of cultivation would decrease the crystallinity of bacterial cellulose. The reason is assumed to be the dense network of cellulose formed by bacterial cells that restrict the motion of themselves as the incubation period extends. Though the effect of inoculum age is still unclear, the influence on crystallinity of bacterial cellulose caused by variation of some ordinary culture conditions can be drawn out from data of this work.

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Effect of ethanol on bacterial cellulose production from fructose in continuous culture

Cited by 91 publications

References 18 publications

The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

Cellulose production and cellulose synthase gene detection in acetic acid bacteria

Screening of the common culture conditions affecting crystallinity of bacterial cellulose

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