Effect of ethanol on organic ion transport in rabbit kidney

Kim, Yong Keun; Lee, Sang Ho; Goldinger, J. M.; Hong, Suk Ki

doi:10.1016/0041-008x(86)90368-6

Cited by 22 publications

(12 citation statements)

References 20 publications

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“…The Na þ , K þ -ATPase activity in the supernatant of the homogenates was determined by measuring inorganic phosphate (Pi) released by ATP hydrolysis during incubation of homogenates with an appropriate medium containing 3 mM ATP (Sigma) as the substrate. 16 The total ATPase activity was determined in the presence of 100 mM Na þ , 20 mM K þ , 3 mM Mg 2þ , 30 mM Tris HCl, 2 mM EDTA (pH 7.4). The Mg 2þ -ATPase activity was determined in the absence of K þ and in the presence of 1 mM ouabain.…”

Section: Measurement Of Namentioning

confidence: 99%

Amelioration of methotrexate‐induced enteritis by melatonin in rats

Jahovic

Şener

Çevik

et al. 2004

Cell Biochemistry & Function

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The anti-tumour drug methotrexate (MTX) induces intestinal mucosa injury resulting in malabsorption and diarrhoea. The purpose of this study was to investigate whether exogenous melatonin could protect the gut from MTX-induced damage in rats. A single dose of MTX (20 mg kg(-1), i.p.) was followed by i.p. saline or melatonin injections (10 mg kg(-1), MTX + Mel) for the next 5 days. On the fifth day, intestinal transit was assessed using charcoal propagation. Rats were decapitated and small intestinal segments were fixed for light (LM) and scanning electron microscope (SEM) examinations. Other intestinal segments were stored to measure glutathione (GSH) and malondialdehyde (MDA) levels, myeloperoxidase (MPO) and ATPase activity. MTX led to loss of more than 10% of the initial body weight (p < 0.01). Conversely, weight loss was markedly less in the melatonin-treated MTX group (p < 0.05). Bowel motility was increased in MTX-treated rats, while the transit index in the MTX-Mel group was not different from the control group. MTX caused decreases in GSH levels and ATPase activity, with increases in MDA levels and MPO activity. These changes were reversed in MTX-Mel-treated rats (p < 0.05-p < 0.001). LM and SEM in the MTX group revealed desquamation of surface epithelium and glandular degeneration, while the epithelium was slightly damaged in the MTX-Mel group. In conclusion, the present study demonstrates that melatonin is capable of reversing MTX-induced intestinal dysfunctions, indicating that it may be beneficial in ameliorating the symptoms of chemotherapy-induced enteritis.

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Section: Measurement Of Namentioning

confidence: 99%

Amelioration of methotrexate‐induced enteritis by melatonin in rats

Jahovic

Şener

Çevik

et al. 2004

Cell Biochemistry & Function

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“…The total ATPase activity was determined in the presence of 100 mM NaCl, 5 mM KCl, 6 mM MgCl 2 , 0.1 mM EDTA, 30 mM Tris HCl (pH 7.4), while the Mg 2+ -ATPase activity was determined in the presence of 1mM ouabain. The difference between the total and the Mg 2+ -ATPase activities was taken as a measure of the Na + -K + -ATPase activity (16,17). The reaction was initiated with the addition of the homogenate (0.1 ml) and a 5-min preincubation period.…”

Section: Myeloperoxidase Activitymentioning

confidence: 99%

Etanercept protects remote organ damage in a rat model of thermal injury

Şehirli¹

2011

mpj

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“…The renal cortical microsomal Na þ -K þ -ATPase activity was measured as described previously (11). The microsomal fraction was prepared from cortex of postischemic kidneys.…”

Section: Analysis and Calculationmentioning

confidence: 99%

Effect of Pentoxifylline on Ischemic Acute Renal Failure in Rabbits

et al. 2001

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Previous studies have demonstrated that levels of tumor necrosis factor-alpha (TNF-alpha) or its mRNA expression are increased in acute renal failure of various types including ischemia/reperfusion injury. This study was undertaken to determine whether pentoxifylline (PTX), an inhibitor of TNF-alpha production, provides a protective effect against ischemic acute renal failure in rabbits. Renal ischemia was induced by clamping bilateral renal arteries for 60 min. Animals were pretreated with PTX (30 mg/kg, i.v.) 10 min before release of clamp. At 24 h of reperfusion of blood after ischemia, changes in renal function, renal blood flow, and the expression of TNF-alpha mRNA were evaluated. Ischemia/reperfusion caused a marked reduction in GFR, which was accompanied by an increase of serum creatinine levels. Such changes were significantly attenuated by PTX pretreatment. PTX ameliorated the impairment of renal tubular function, but it had no effect on the reduction of renal blood flow induced by ischemia/reperfusion. The protective effect of PTX on functional changes was supported by morphological studies. The impairment of glucose and phosphate reabsorption in postischemic kidneys was associated with a depression in the expression of Na+-glucose and Na+-Pi transporters. The expression of TNF-alpha mRNA was increased after reperfusion, which was inhibited by PTX pretreatment. The PTX pretreatment in vitro prevented the release of lactate dehydrogenase induced by an oxidant t-butylhydroperoxide in rabbit renal cortical slices, but it did not produce any effect on the oxidant-induced lipid peroxidation, suggesting that PTX protection is not resulted from its antioxidant action. These results suggest that PTX may exert a protective effect against ischemic acute renal failure by inhibiting the production of TNF-alpha in rabbits.

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Effect of ethanol on organic ion transport in rabbit kidney

Cited by 22 publications

References 20 publications

Amelioration of methotrexate‐induced enteritis by melatonin in rats

Amelioration of methotrexate‐induced enteritis by melatonin in rats

Etanercept protects remote organ damage in a rat model of thermal injury

Effect of Pentoxifylline on Ischemic Acute Renal Failure in Rabbits

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