Effect of ethyl pyruvate on oxidative state and endoplasmic reticulum stress in a rat model of testicular torsion

Demir, Selim; Kazaz, İlke Onur; Aliyazıcıoğlu, Yüksel; Keri̇moğlu, Gökçen; Teoman, Ahmet Serdar; Yaman, Serap Özer; Arslan, Ayhan; Menteşe, Ahmet

doi:10.1080/10520295.2019.1695947

Cited by 21 publications

(9 citation statements)

References 33 publications

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“…Finally, ethyl Pyr (a derivative of sodium salt of Pyr) was recently shown to have an inhibitive effect on ER stress, in vivo , in a rat model [36], which strongly supported the present findings, in vitro , but ethyl Pyr does not work in humans [37].…”

Section: Discussionsupporting

confidence: 88%

Pyruvate alleviates high glucose‐induced endoplasmic reticulum stress and apoptosis in HK‐2 cells

et al. 2020

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Endoplasmic reticulum (ER) stress plays a critical role in the development of diabetic nephropathy (DN). We previously demonstrated that pyruvate (Pyr)‐enriched oral rehydration solution improved glucometabolic disorders and ameliorated DN outcome in db/db mice. Here, we investigated the effects of Pyr on high glucose‐induced ER stress and apoptosis in HK‐2 cells. Our results suggest that high glucose can induce reactive oxygen species production, apoptosis and ER stress in HK‐2 cells, and that Pyr treatment can ameliorate these effects and restore the expression of key proteins involved in ER stress. Thus, Pyr may have potential for the development of novel strategies for the prevention and treatment of clinical DN.

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Section: Discussionsupporting

confidence: 88%

Pyruvate alleviates high glucose‐induced endoplasmic reticulum stress and apoptosis in HK‐2 cells

et al. 2020

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“…Many previous studies have shown that it is possible to improve the prognosis and protect testicular tissue and spermatogenic function by improving the oxidative stress injury caused by ischemiareperfusion after testicular torsion reduction [34]. In this study, we con rmed that the exosome derived from bone marrow mesenchymal stem cells can reach around the seminiferous tubule through the bloodtestis barrier of rats, and further con rmed that exosome derived from bone marrow mesenchymal stem cells can reduce the degree of oxidative stress injury in testicular tissue and improve testicular ischemiareperfusion injury by HE staining and the content of related oxidative stress indexes.…”

Section: Discussionmentioning

confidence: 99%

“…Testicular torsion (TT), is a testicular torsion along the longitudinal axis of the spermatic cord [1], resulting in acute reduction or interruption of testicular blood supply, resulting in testicular tissue ischemia and necrosis. The incidence of testicular torsion is up to 158, which mostly occurs in teenagers under 24 years old [2][3][4][5][6]. Clinically, because it is di cult to distinguish the disease from scrotal in ammation in the early stage [1,2,7,8], domestic data show that the misdiagnosis rate of testicular torsion can be as high as 67.6%.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Tian

Zhang

Yang

et al. 2021

Preprint

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Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group. Conclusion BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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“…Many previous studies have shown that it is possible to improve the prognosis and protect testicular tissue and spermatogenic function by improving the oxidative stress injury caused by ischemia-reperfusion after testicular torsion reduction [34]. In this study, we con rmed that the exosome derived from bone marrow mesenchymal stem cells can reach around the seminiferous tubule through the bloodtestis barrier of rats, and further con rmed that exosome derived from bone marrow mesenchymal stem cells can reduce the degree of oxidative stress injury in testicular tissue and improve testicular ischemiareperfusion injury by HE staining and the content of related oxidative stress indexes.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of BMSCs-derived Exosomes on Testicular Ischemia-reperfusion Injury in Rats

Tian

Zhang

Yang

et al. 2021

Preprint

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Background: Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury.Methods: BMSCs were isolated, cultured and identified by primary culture method. Exosomes derived from BMSCs were extracted by ultra-high speed centrifugation method. A testicular IRI model was established in male SD rats. Thirty healthy male SD rats were randomly divided into three groups. Group A: Sham group, group B: normal saline treatment group (I/R+NS), group C: BMSCs-derived exosomes (100 ug/mL) treatment group (I/R+ BMSCs-EXO). Finally take each side (left) of rat torsion by using optical microscope to detect testicular tissue pathology grade and fine structure of organization structure, adopt the method of biochemical determination of groups of testicular tissue MDA and NOS, SOD and CAT activity and T - AOC level.Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In animal model, Compared with the normal spermatogenic structure of testis in group A (Sham), the spermatogenic structure of testis in group B (I/R+NS) was obviously damaged to varying degrees, while the spermatogenic structure of testis in group C (I/R+ BMSCs-EXO) was improved to A certain extent (P<0.05). In the biochemical indexes of testis tissue, the contents of MDA and NOS in group B (I/R+NS) were significantly increased compared with group A (Sham), while the activities of SOD and CAT and T-AOC were decreased compared with group A (Sham) (P<0.05). In the exosome-treated group C (I/R+ BMSCs-EXO), compared with the normal saline treatment group B, The contents of MDA and NOS were decreased to a certain extent, while the activities of SOD and CAT and the level of T-AOC were increased (P<0.05).Conclusion: BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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Effect of ethyl pyruvate on oxidative state and endoplasmic reticulum stress in a rat model of testicular torsion

Cited by 21 publications

References 33 publications

Pyruvate alleviates high glucose‐induced endoplasmic reticulum stress and apoptosis in HK‐2 cells

Pyruvate alleviates high glucose‐induced endoplasmic reticulum stress and apoptosis in HK‐2 cells

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Protective Effect of BMSCs-derived Exosomes on Testicular Ischemia-reperfusion Injury in Rats

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