Effect of Eucommia water extract on gingivitis and periodontitis in experimental rats

Wang, Yueyue; Fan, Qin; Xu, Yanglong; Zeng, Fengjiao; Liu, Xia; Zhao, Dan; Zhang, Lei; Bai, Guohui

doi:10.1186/s12903-022-02353-5

Cited by 3 publications

(4 citation statements)

References 20 publications

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“…The outcomes of our study illustrated that the bone loss detected in rats undergoing dental ligation, as evidenced by both methylene blue staining and radiographic analysis, faithfully recreated the periodontitis model. These findings are consistent with earlier research, which affirms the successful establishment of a periodontitis model through the induction of alveolar bone resorption (20,31,51).…”

Section: Top Of Formsupporting

confidence: 93%

Effect of experimental periodontitis on cardiac functions: a comprehensive study using echocardiography, hemodynamic analysis, and histopathological evaluation in a rat model

Elhaieg,

Farag,

Elfadadny

et al. 2023

Front. Vet. Sci.

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IntroductionPeriodontitis is a prevalent and severe dental condition characterized by the gradual degradation of the bone surrounding the teeth. Over the past two decades, numerous epidemiological investigations have suggested a potential link between periodontitis and cardiovascular disease. However, the complex mechanistic relationship between oral health issues and cardiovascular disorders remains unclear.AimThis study aimed to explore comprehensively the cardiac function through various methods, including conventional echocardiography, intraventricular pressure gradient (IVPG) analysis, speckle tracking echocardiography (STE), and hemodynamics analysis.MethodsLigature-induced periodontitis was established in a group of rats while the second group served as sham. The successful establishment of the periodontitis model was confirmed through staining and radiographic examination of the affected mandibles.ResultsX-ray films and methylene blue staining revealed alveolar bone resorption in the affected first molar in the model rats, confirming the successful induction of periodontitis. The rats with periodontitis displayed a decrease in ejection fraction compared to the sham group, accompanied by a decrease in mid-to-apical IVPG and mid IVPG. Lower values of strain rate were recorded in the apical segment of the septum, the middle segment of the septum, and the basal segment of the lateral free wall in the periodontitis group, which was associated with histopathological examination showing some degree of myocardial tissue damage. Conversely, rats with periodontitis showed an increase in heart rate, end-systolic volume, and arterial elastance when compared to the sham rats. However, they also exhibited a decrease in stroke work, stroke volume, cardiac output, and end-systolic pressure.ConclusionThis study suggests that experimental periodontitis may lead to cardiac dysfunction especially compromised systolic function and myocardial relaxation, potentially indicating an increased risk of cardiovascular events in clinical periodontitis cases. The comprehensive assessment of cardiac function, hemodynamics, and histopathological evaluation underscores the profound impact of periodontitis on heart functions within this specific experimental model.

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Section: Top Of Formsupporting

confidence: 93%

Effect of experimental periodontitis on cardiac functions: a comprehensive study using echocardiography, hemodynamic analysis, and histopathological evaluation in a rat model

Elhaieg,

Farag,

Elfadadny

et al. 2023

Front. Vet. Sci.

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“…periodontitis model was successfully established after four weeks, with reference to the previous report (Figure 2) [33].…”

Section: Induction Of Periodontitissupporting

confidence: 55%

“…To ensure that the thread on the first molar did not fall off, the “figure-of-eight ligation” was used ( Figure 2 ). The periodontitis model was successfully established after four weeks, with reference to the previous report ( Figure 2 ) [ 33 ].…”

Section: Induction Of Periodontitismentioning

confidence: 86%

Initial Development of an Immediate Implantation Model in Rats and Assessing the Prognostic Impact of Periodontitis on Immediate Implantation

et al. 2023

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Background: To establish an immediate implantation rat model and to evaluate the effects of pre-existing periodontitis and two different socket rinse solutions on immediate implantation prognosis. Methods: Sprague-Dawley (SD) rats were randomly divided into three groups before immediate implantation, including the control group, the group with experimentally induced periodontitis (EP), in which rats have been experimentally induced periodontitis before implantation, and the group with induced periodontitis and with extraction sockets rinsed with three percent H2O2 (EP-H2O2), in which rats have been induced periodontitis before implantation, and extraction sockets were rinsed with three percent H2O2. Periodontitis was induced by ligating the thread around the molars for four weeks. Six weeks after titanium alloy implants were self-tapped and left to heal transmucosally, maxillae were dissected after the clinical examination to perform micro-CT and histological analysis. Results: An immediate implantation model was successfully built in rats. There was no significant difference in implant survival rates between the EP and control groups. However, the clinical examination results, micro-CT analysis, and histological analysis in EP and EP-H2O2 groups showed a significantly worse prognosis than in the control group. Three percent H2O2 showed a similar effect with saline. Conclusion: This study presented a protocol for establishing a rat immediate implantation model and showed that periodontitis history might negatively affect the prognosis of immediate implantation. These findings urge caution and alternative strategies for patients with periodontal disease history, enhancing the long-term success of immediate implantation in dental practice. Additionally, the comparable outcomes between 3% H2O2 and saline suggest the use of saline as a cost-effective and safer alternative for implant site preparation in dental practice.

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“…Male rodents had their second maxillary molars ligated and were fed moist food and 10% sucrose water. Two weeks later, the gingival inflammation model was effectively established [8]. Not much has been research regarding the induction of periodontal diseases in pregnant rats.…”

Section: Introductionmentioning

confidence: 99%

Gingival inflammation induction in pregnant Sprague-Dawley rats: A pilot study

Ani Rahman,

Sunarintyas,

Martien

et al. 2023

BIO Web Conf.

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Pregnancy gingivitis is an inflammation of the gingiva caused by dental plaque and exacerbated by hormonal changes. Gingival inflammation is often induced in laboratory animal models, such as SpragueDawley rats used for experimental models. This investigation was to establish inducing gingival inflammation in pregnant rats. The purpose of this study was to establish a reproducible method for inducing gingival inflammation in both incisive mandibles of pregnant Sprague-Dawley rats using ligation and dietary manipulation. Pregnant Sprague-Dawley rats were used as experimental subjects. As ligation, 4-0 nonresorbable silk thread was utilized and affixed using a “8”-shaped knot technique. The ligatures were inserted between the mandible’s central incisors. The operation was carried out under anaesthesia. Once the rat’s ligature was removed after 5th,7th,10th and 14th days. The clinical appearance and radiographic examination were evaluated. Gingival inflammation induction by ligation and dietary intake modification caused inflammation of gingival tissue, was seen at 5th day. Clinical examination showed getting worst at 14th day. In our study, gingival inflammation on pregnant rat was achieved five days after ligation and dietary intake modification.

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Effect of Eucommia water extract on gingivitis and periodontitis in experimental rats

Cited by 3 publications

References 20 publications

Effect of experimental periodontitis on cardiac functions: a comprehensive study using echocardiography, hemodynamic analysis, and histopathological evaluation in a rat model

Effect of experimental periodontitis on cardiac functions: a comprehensive study using echocardiography, hemodynamic analysis, and histopathological evaluation in a rat model

Initial Development of an Immediate Implantation Model in Rats and Assessing the Prognostic Impact of Periodontitis on Immediate Implantation

Gingival inflammation induction in pregnant Sprague-Dawley rats: A pilot study

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