Effect of Eugenia Jambolana on number of inflammatory cells in gingivitis in albino rats.

Abstract

Objectives: This study was conducted to study the effect of Eugenia jambolana extract on the thickness of epithelium in albino rats. Study Design: This experimental study. Setting: Conducted in the animal house, Anatomy Department, Post Graduate Medical Institute, Lahore, Pakistan. Period: July to August 2015. Material and Methods: Total 48 albino rats were taken in this study and were divided into three groups. Histological changes of the buccal mucosa were studied on 3rd, 4th, 10th and 20th day after the induction of gingivitis in both control and experimental group. Results: On day 3, the cellular infiltrate was three times in both experimental groups. The total count of neutrophils was 22.50±2.082, eosinophils was 50.00±1.414 in group 1B. In group 1C, the neutrophils count was 27.50±1.915, eosinophil count was 5.00±0.816. On day 4, in group-2B, no eosinophils were seen. Lymphocytes was 19.75±1.708, neutrophils was 6.50±1.219 and macrophages was 3.25±1.258. In group 2C, lymphocytes and macrophages present, very few neutrophils were also seen. Some fibroblast nuclei were seen. In group 2C, no eosinophils were seen. The lymphocytes count was 9.00±1.155, neutrophil count was 4.00±1.414 and macrophage count was 1.25±0.050. On day 10, lymphocytes, plasma cells, macrophages and few neutrophils were seen. In group3B, macrophages with large nucleus 3.25±0.95 lymphocytes 1.25 ±0.500 and few neutrophils 1.00±0.000 were also present. No eosinophils were present. In group 3C, less inflammatory cells (table 4) beneath the epithelium. The subepithelial tissue consisted of more lymphocytes 1.75±0.500 and neutrophils 1.25±0.500.On day 20, very few lymphocytes 1.75±0.500 and neutrophils 1.50±0.577 in experimental group 4B. In group 4C, lymphocytes 1.75±0.500 and neutrophils 1.50±0.577 were present, no evidence of macrophages and eosinophils in group 4B and 4C. Conclusion: This study proved the beneficial effects of Eugenia jambolana on the healing of gingivitis. The contents in Eugenia jambolana have an anti-inflammatory action on soft tissues.