Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle

Benziane, Boubacar; Widegren, Ulrika; Pirkmajer, Sergej; Henriksson, Jan; Stepto, Nigel K.; Chibalin, Alexander V.

doi:10.1152/ajpendo.00533.2010

Cited by 33 publications

(66 citation statements)

References 53 publications

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“…We have previously demonstrated, using a phosphorylation-specific anti-PLM antibody, that running had no influence on PLM phosphorylation state in rats (24). In contrast to these data, exercise increases PLM phosphorylation in human skeletal muscle (1,27). The present data fit with the suggestion that binding of unphosphorylated PLM is increased after exercise; however, there is a lack of information regarding the differences in the way PLM interacts with different ␣␤ dimers.…”

Section: Discussionmentioning

confidence: 90%

Na⁺-K⁺-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Juel¹

2009

American Journal of Physiology-Regulatory, Integrative and Comp

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Section: Discussionmentioning

confidence: 90%

Na⁺-K⁺-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Juel¹

2009

American Journal of Physiology-Regulatory, Integrative and Comp

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“…PLM, a member of FXYD family, is a regulator of the Cav1.2 and Ca 2+ influx36. In rat myocytes, PLM over-expression alters contractility and cytoslic [Ca 2+ ] i transients37.…”

Section: Discussionmentioning

confidence: 99%

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system

Liang

Wang

et al. 2017

Sci Rep

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Dysregulation of intracellular trafficking system plays a fundamental role in the progression of cardiovascular disease. Up-regulation of miR-1 contributes to arrhythmia, we sought to elucidate whether intracellular trafficking contributes to miR-1-driven arrhythmia. By performing microarray analyses of the transcriptome in the cardiomyocytes-specific over-expression of microRNA-1 (miR-1 Tg) mice and the WT mice, we found that these differentially expressed genes in miR-1 Tg mice were significantly enrichment with the trafficking-related biological processes, such as regulation of calcium ion transport. Also, the qRT-PCR and western blot results validated that Stx6, Braf, Ube3a, Mapk8ip3, Ap1s1, Ccz1 and Gja1, which are the trafficking-related genes, were significantly down-regulated in the miR-1 Tg mice. Moreover, we found that Stx6 was decreased in the heart of mice after myocardial infarction and in the hypoxic cardiomyocytes, and further confirmed that Stx6 is a target of miR-1. Meanwhile, knockdown of Stx6 in cardiomyocytes resulted in the impairments of PLM and L-type calcium channel, which leads to the increased resting ([Ca2+]i). On the contrary, overexpression of Stx6 attenuated the impairments of miR-1 or hypoxia on PLM and L-type calcium channel. Thus, our studies reveals that trafficking-related gene Stx6 may regulate intracellular calcium and is involved in the occurrence of cardiac arrhythmia, which provides new insights in that miR-1 participates in arrhythmia by regulating the trafficking-related genes and pathway.

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“…This effect is partly mediated by a decrease in K m for Na ϩ (increased Na ϩ affinity) (9). In addition, exercise has been shown to increase the association between the ␣ and PLM subunits in rat (17), and exercise in humans has been demonstrated to increase the phosphorylation of the PLM unit (3,21), which is known to modify the Na-K-ATPase ion affinity (13). Therefore, exercise-induced PLM phosphorylation and the resulting affinity changes are likely involved in the exercise-induced increase in ATPase activity revealed with the ATPase assay in the present study.…”

Section: Discussionmentioning

confidence: 99%

Exercise-induced increase in maximal in vitro Na-K-ATPase activity in human skeletal muscle

Juel

Nordsborg

Bangsbo

2013

American Journal of Physiology-Regulatory, Integrative and Comp

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The present study investigated whether maximal in vitro Na-K-ATPase activity in human skeletal muscle is changed with exercise and whether it was altered by acute hypoxia. Needle biopsies from 14 subjects were obtained from vastus lateralis before and after 4 min of intense muscle activity. In addition, six subjects exercised also in hypoxia (12.5% oxygen). The Na-K-ATPase assay revealed a 19% increase (P < 0.05) in maximal velocity (Vmax) for Na⁺-dependent Na-K-ATPase activity after exercise and a tendency (P < 0.1) toward a decrease in Km for Na⁺ (increased Na⁺ affinity) in both normoxia and hypoxia. In contrast, the in vitro Na-K-ATPase activity determined with the 3-O-MFPase technique was 11-32% lower after exercise in normoxia (P < 0.05) and hypoxia (P < 0.1). Based on the different results obtained with the Na-K-ATPase assay and the 3-O-MFPase technique, it was suggested that the 3-O-MFPase method is insensitive to changes in Na-K-ATPase activity. To test this possibility, changes in Na-K-ATPase activity was induced by protein kinase C activation. The changes quantified with the Na-K-ATPase assay could not be detected with the 3-O-MFPase method. In addition, purines stimulated Na-K-ATPase activity in rat muscle membranes; these changes could not be detected with the 3-O-MFPase method. Therefore, the 3-O-MFPase technique is not sensitive to changes in Na⁺ sensitivity, and the method is not suited to detecting changes in Na-K-ATPase activity with exercise. In conclusion, muscle activity in humans induces an increased in vitro Na⁺-dependent Na-K-ATPase activity, which contributes to the upregulation of the Na-K-ATPase in association with exercise both in normoxia and hypoxia.

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Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle

Abstract: Benziane B, Widegren U, Pirkmajer S, Henriksson J, Stepto NK, Chibalin AV. Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

Cited by 33 publications

References 53 publications

Na⁺-K⁺-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Na⁺-K⁺-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system

Exercise-induced increase in maximal in vitro Na-K-ATPase activity in human skeletal muscle

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Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle

Abstract: Benziane B, Widegren U, Pirkmajer S, Henriksson J, Stepto NK, Chibalin AV. Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

Cited by 33 publications

References 53 publications

Na+-K+-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Na+-K+-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system

Exercise-induced increase in maximal in vitro Na-K-ATPase activity in human skeletal muscle

Contact Info

Product

Resources

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Na⁺-K⁺-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity

Na⁺-K⁺-ATPase in rat skeletal muscle: muscle fiber-specific differences in exercise-induced changes in ion affinity and maximal activity