Effect of exercise training on Ca2+ release units of left ventricular myocytes of spontaneously hypertensive rats

Carneiro‐Júnior, Miguel Araujo; Quintão-Júnior, Judson Fonseca; Drummond, Lucas Rios; Lavorato, Victor Neiva; Drummond, Filipe Rios; Amadeu, Marco Aurélio; Oliveira, Eleonora Menicucci de; Félix, Leonardo Bonato; Cruz, Jáder Santos; Mill, José Geraldo; Natali, Antônio José; Prímola-Gomes, Thales Nicolau

doi:10.1590/1414-431x20144063

Cited by 17 publications

(14 citation statements)

References 20 publications

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“…Our results indicate that training reduced the deleterious effects of hypertension by decreasing calcium leakage through ryanodine receptor (decrease in spark site density for HIIE) and by improving calcium uptake, resulting in temporal and spatial shortening of sparks (FDHM and FWHM were decreased). To our knowledge, only one study on the effect of training on spontaneous calcium release during hypertension has been published (12). For the four parameters studied previously, however, the reported results of this study are contradictory with ours: an increase in amplitude, FDHM, FWHM, and peak time after 8 wk of training was observed.…”

Section: Discussioncontrasting

confidence: 99%

High-intensity intermittent training is as effective as moderate continuous training, and not deleterious, in cardiomyocyte remodeling of hypertensive rats

Krzesiak

Cognard

Sebille

et al. 2019

Journal of Applied Physiology

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Exercise training offers possible nonpharmacological therapy for cardiovascular diseases including hypertension. High-intensity intermittent exercise (HIIE) training has been shown to have as much or even more beneficial cardiovascular effect in patients with cardiovascular diseases than moderate-intensity continuous exercise (CMIE) training. The aim of this study was to investigate the effects of the two types of training on cardiac remodeling of spontaneously hypertensive rats (SHR) induced by hypertension. Eight-week-old male SHR and normotensive Wistar-Kyoto rats (WKY) were divided into four groups: normotensive and hypertensive control (WKY and SHR-C) and hypertensive trained with CMIE (SHR-T CMIE) or HIIE (SHR-T HIIE). After 8 wk of training or inactivity, maximal running speed (MRS), arterial pressure, and heart weight were all assessed. CMIE or HIIE protocols not only increased final MRS and left ventricular weight/body weight ratio but also reduced mean arterial pressure compared with sedentary group. Then, left ventricular tissue was enzymatically dissociated, and isolated cardiomyocytes were used to highlight the changes induced by physical activity at morphological, mechanical, and molecular levels. Both types of training induced restoration of transverse tubule regularity, decrease in spark site density, and reduction in half-relaxation time of calcium transients. HIIE training, in particular, decreased spark amplitude and width, and increased cardiomyocyte contractility and the expression of sarco(endo)plasmic reticulum Ca2+-ATPase and phospholamban phosphorylated on serine 16. NEW & NOTEWORTHY High-intensity intermittent exercise training induces beneficial remodeling of the left ventricular cardiomyocytes of spontaneously hypertensive rats at the morphological, mechanical, and molecular levels. Results also confirm, at the cellular level, that this type of training, as it appears not to be deleterious, could be applied in rehabilitation of hypertensive patients.

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Section: Discussioncontrasting

confidence: 99%

High-intensity intermittent training is as effective as moderate continuous training, and not deleterious, in cardiomyocyte remodeling of hypertensive rats

Krzesiak

Cognard

Sebille

et al. 2019

Journal of Applied Physiology

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“…Such increase in physical capacity in response to aerobic training has been previously demonstrated. 10 , 17 , 18 More importantly, physical training reduced SAP in hypertensive animals, and such effect is well established in the literature. 11 , 19 …”

Section: Discussionmentioning

confidence: 58%

Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats

Rodrigues¹,

Prímola-Gomes²,

Soares³

et al. 2018

Arquivos Brasileiros De Cardiologia

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BackgroundRegulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals.Objective To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR).MethodsSHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons.ResultsExercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only.ConclusionThe aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility.

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“…The impact of exercise and physical activity on the progression of cardiac aging is also an active area of research. There is evidence in both healthy animal models and models of other cardiac disease phenotypes that exercise can induce alterations in cardiac Ca 2+ cycling [175,176,177]. Endurance exercise attenuated increased Ca 2+ spark frequency, CaMKII-mediated RyR2 phosphorylation at Serine-2814, and Ca 2+ alternans in a dog model of sudden cardiac death, which reduced ischemically-induced VF [175].…”

Section: Perspectivementioning

confidence: 99%

“…Long-term interval training also reduced CaMKII-dependent phosphorylation of RyR2 and the incidence of VT in a mouse model of CPVT [176]. In spontaneously hypertensive rats, endurance exercise training reversed increased RyR2 expression and attenuated the proarrhythmic increase in Ca 2+ spark activity in left ventricular myocytes [177]. In the context of the aging heart, research on the effects of exercise on intracellular Ca 2+ homeostasis remains limited.…”

Section: Perspectivementioning

confidence: 99%

Altered Intracellular Calcium Homeostasis and Arrhythmogenesis in the Aged Heart

Hamilton

Terentyev

2019

IJMS

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Aging of the heart is associated with a blunted response to sympathetic stimulation, reduced contractility, and increased propensity for arrhythmias, with the risk of sudden cardiac death significantly increased in the elderly population. The altered cardiac structural and functional phenotype, as well as age-associated prevalent comorbidities including hypertension and atherosclerosis, predispose the heart to atrial fibrillation, heart failure, and ventricular tachyarrhythmias. At the cellular level, perturbations in mitochondrial function, excitation-contraction coupling, and calcium homeostasis contribute to this electrical and contractile dysfunction. Major determinants of cardiac contractility are the intracellular release of Ca2+ from the sarcoplasmic reticulum by the ryanodine receptors (RyR2), and the following sequestration of Ca2+ by the sarco/endoplasmic Ca2+-ATPase (SERCa2a). Activity of RyR2 and SERCa2a in myocytes is not only dependent on expression levels and interacting accessory proteins, but on fine-tuned regulation via post-translational modifications. In this paper, we review how aberrant changes in intracellular Ca2+ cycling via these proteins contributes to arrhythmogenesis in the aged heart.

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Effect of exercise training on Ca2+ release units of left ventricular myocytes of spontaneously hypertensive rats

Cited by 17 publications

References 20 publications

High-intensity intermittent training is as effective as moderate continuous training, and not deleterious, in cardiomyocyte remodeling of hypertensive rats

High-intensity intermittent training is as effective as moderate continuous training, and not deleterious, in cardiomyocyte remodeling of hypertensive rats

Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats

Altered Intracellular Calcium Homeostasis and Arrhythmogenesis in the Aged Heart

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