Vegetable oils as fuel have received attention in recent years due to their advantages including renewable nature and domestically produced energy resources. Achieving reduced emission is important subject, as it enhances sustainability of biofuels. General factorial experimental design and analysis of variance (ANOVA) is used to analyze how NOx emission is influenced by alcohol content in vegetable oil-diesel fuel blends. Fuel samples 20% croton oil -80% diesel (20%CRO-80%D2), 15% croton oil -5% 1-butanol -80% diesel (15%CRO-5%BU-80%D2), and 10% croton oil -10% 1-butanol -80% diesel (10%CRO-10%BU-80%D2) were investigated. Four-cylinder compression-ignition (CI) engine was run at constant speed 3000 RPM, loaded at different loading conditions: low idle as 0 to 100% load condition. The specific fuel consumption and exhaust temperature of fuel blend containing butanol was measured. It was observed that nitrogen oxides (NOx) emission decreases as percentage of butanol alcohol content increases in fuel samples.