Effect of exogenous cycllc amp on catabolite repression of cellulase formation in aspergillus nidulans

Bagga, P. S.; Sandhu, D. K.; Sharma, Sunita

doi:10.1002/abio.370110419

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…The combined extracts lost no activity suggesting incapability of proteases to inactivate both glucanases. , WOOD et al 1984SIDHU and SANDHU 1985, KALRA et al 1986, BAGGA et al 1989. Thus, in common with other cellulolytic microorganisms, S. albaduuncus utilizes cellulose for growth and regulates cellulase biosynthesis by feedback control in the presence of glycerol by underlying the phenomenon of catabolite rcprcssion.…”

Section: Resultsmentioning

confidence: 91%

Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus

Harchand

Singh

1994

J. Basic Microbiol.

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The cellulase complex of S. albaduncus was found to undergo catabolite repression in the presence of glycerol accompanied by a decrease in the pH of the medium. The results of the experiments conducted at two different initial pH of the medium suggested that pH effect as well as proteases were involved in the inactivation of glucanases (exoglucanase and endoglucanase) whereas beta-glucosidase inactivation was solely due to 'pH effect'. It was also apparent that pH change followed by the addition of glycerol caused alterations in protease activities as well as pattern of their production.

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Section: Resultsmentioning

confidence: 91%

Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus

Harchand

Singh

1994

J. Basic Microbiol.

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“…This study substantiates the work of Nochure et al (1993) who demonstrated fructose as the best inducer of Avicellase in Clostridium thermocellum. Bagga et al (1989) identified lactose as the best inducer of endoglucanase and cellobiohydolase. Trehalose has been demonstrated as the best inducer of cellulases in a Clostridium sp.…”

Section: Evaluation Of Carbon Sources For Induction/repression Of Glumentioning

confidence: 98%

Induction, and production studies of a novel glucoamylase of Aspergillus niger

Rajoka

Yasmeen

2005

World J Microbiol Biotechnol

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The influence of carbon and nitrogen sources on the production of a raw-starch-digesting glucoamylase was investigated. The enzyme production was variable according to the carbon source. Levels of glucoamylase were minimal in the presence of even low concentrations of glucose while its production was stimulated by other carbohydrates. Wheat bran and cellulose were the most effective inducers of glucoamylase activities, followed by rice bran. Exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of A. niger growing on wheat bran. In defined medium with maltose, the glucoamylase titres were 5.2-to 16.7-fold higher with cells growing on monomeric sugars and 1.5 times higher than cells growing on other disaccharides. It appeared that synthesis of glucoamylase varied under an induction mechanism, and a repression mechanism which changed the rate of synthesis of enzyme in induced over non-induced cultures. In this organism, substantial synthesis of glucoamylase could be induced by maltose, cello-dextrin, cellulose or cellulose-and hemicellulose-containing substrates which showed low volumetric substrate uptake rate. During growth of A. niger on wheat bran, maximum volumetric productivity (Q p ) of glucoamylase was 274 IU l )1 h )1 and is significantly higher than the values reported for some other potent fungi. The addition of actinomycin D (a repressor of transcription) and cycloheximide, (a repressor of translation) completely repressed glucoamylase biosynthesis, suggested that the regulation of glucoamylase synthesis in this organism occurs at both transcriptional and translational level. Thermodynamic studies revealed that the culture exerted protection against thermal inactivation when exposed to different fermentation temperatures.

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“…Also, catabolite repression of cellulase formation in fungi has been reported frequently. For instance, Bagga, Sandhu & Sharma (1989) demonstrated that the addition of glucose to a medium containing CMC Significantly repressed all com ponents of the cellulase complex of AspergillUS' nidulans. Horton & Keen (1966) showed that 500 I-lM glucose was sufficient to repress the cellulase of the plant pathogen Pyrenochaeta terrestris.…”

Section: Discussionmentioning

confidence: 99%

Cellulolytic enzymes of the obligately biotrophic rust fungus Uromyces viciae-fabae are regulated differentiation-specifically

Heiler¹,

Mendgen²,

Deising³

1993

Mycological Research

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Uredospores of the obUgately biofrophic broad bean rust fungus Uromyces viciae-fabae form infection structures on artificial membranes providing a thigmotropic signal. In nalure these are essential for invasion of the host plant through the stomata. '[his experimental system was used to analyse the production of cellulolytic enzymes duting the differentiation of rust infection stru~lures.Low .cellulase activity was detected in dormant spores and in germ tubes. Enzyme activity increased duting appressorium development and reached a maximum when infection hyphae and haustorial mother cells were formed. Seven cellulolytic enzymes were separated by chromatofocusing on DEAE = Si500 and PBE 94 exchangers. At least two cellulases, characterized by isoelectric points of 7' I and 7'3, were identified as endo-eellulases. The neutral enzyme forms increased from 3'3 % of the total activity at appressorium formation to 36'6% when infection hyphae were formed (18 h p.i.), and to 45'4% in haustorial mother cells (24 h p.i.). Cellulase activity of the rust' fungus is neither substrate-inducible nor catabolite repressible. The regulation of these enzymes appears to be strictly controlled by the differentiation of infection struclures, and is therefore distinct from cellulases of necrotrophs and saprophytes investigated thus far.

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Effect of exogenous cycllc amp on catabolite repression of cellulase formation in aspergillus nidulans

Cited by 9 publications

References 24 publications

Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus

Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus

Induction, and production studies of a novel glucoamylase of Aspergillus niger

Cellulolytic enzymes of the obligately biotrophic rust fungus Uromyces viciae-fabae are regulated differentiation-specifically

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