Effect of exogenous fibrolytic enzyme on ruminal fermentation and digestibility of alfalfa and rye-grass hay fed to lambs

Abstract

This experiment was carried out to study the effect of a directly fed exogenous fibrolytic enzyme on intake and digestion of DM, OM, protein, NDF, ADF, and hemicellulose of alfalfa and ryegrass hay by sheep. Four diets were randomly assigned to four ruminally cannulated lambs using a 4 x 4 Latin square design, repeated in time, with a factorial arrangement (2 x 2) of diets: 1) alfalfa hay; 2) alfalfa hay + exogenous fibrolytic enzymes (enzyme); 3) ryegrass hay; and 4) ryegrass hay + enzyme. Lambs consumed more DM and OM from alfalfa than from ryegrass hay (P < 0.001). The ADF intake was not different between the hays, but NDF intake was lower for alfalfa (P < 0.001). For both hays, the enzyme increased intake of DM (P < 0.01), as well as OM and CP (P < 0.05); however, NDF and ADF intake were not changed. Alfalfa hay had higher apparent digestibility of DM, OM, and CP (P < 0.001), but lower digestibility for NDF, ADF, and hemicellulose. The enzyme increased apparent digestibility of CP, hemicellulose (P < 0.05), and NDF (P < 0.10) for alfalfa. Also, for both hays, the enzyme improved N balance because lambs retained more N (P < 0.05). The enzyme increased (P < 0.05) total VFA concentration (3 and 6 h) for both hays. Results from this trial indicate that directly fed exogenous fibrolytic enzymes may change ruminal fermentation, intake, and digestibility of forages with different nutritive value.

Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

“…Additionally, an increment of 9% and 10% over in vitro true digestibility of ACS (TDA) was observed after 48 hrs and 72 hrs ruminal digestion, with previous exposure to EE for 12 hrs ( Figure 3b). Similar results were reported for alfalfa hay fed to sheep where a 6.3% increase on dry matter digestibility and 11.7% more hemicellulose digestibility (Pinos-Rodríguez et al 2002). However, addition of commercial fibrolytic enzymes, mainly endoglucanase and xylanase directly fed into sheep's rumen at a rate of 0 or 12 g d -1 , did not correlate with in situ dry matter and NDF degradation of grass hay (Giraldo et al 2008).…”

“…Xylanase extracts may be incorporated as ruminant feed additives, to improve ruminal fiber digestion resulting in increased digestible energy intake (Pinos-Rodríguez et al 2002;Giraldo et al 2008). Furthermore, the demand for xylo-oligosaccharides has increased due to their prebiotic function (Maalej-Achouri et al 2009).…”

Rumen microorganisms and fermentation

“…Also cellulase has been shown to increase protein degradation of forages in vitro, because proteins are more available to proteolytic enzymes (Kohn and Allen 1992). Since intake of fibrolytic enzymes improve the forage digestibility, it increases the total digestible nutrients and consequently changes concentration and proportion of VFA, therefore, ruminant productivity improves (Pinos-Rodríguez et al 2002, Pinos-Rodríguez et al 2008. The enzyme products with this activity used in ruminant nutrition are usually produced by fungi such as Trichoderma longibrachiantum, Aspergillus niger, Penicillium funiculosum (Wallace et al 2001) and A. oryzae, as well as Bacillus spp.…”

Parameters of Protein Metabolism in Goats Fed Diets With Different Portion of Sugarcane Bagasse

“…Intakes of feed dry matter, organic matter, and protein decreased with the increasing of bagasse portion in diets (Table 1). Pinos-Rodriguez et al (2002) stated that increasing bulkiness of diet results in lower feed intake in ruminant. Moreover, greater cell wall content in a diet may cause lesser ruminal degradation, and slower digestion and passage rates (Cantalapiedra-Hijar et al, 2009).…”