Effect of exogenous GA3 on flowering quality, endogenous hormones, and hormone- and flowering-associated gene expression in forcing-cultured tree peony (Paeonia suffruticosa)

Guan, Yanan; Xue, Jingqi; Xue, Yuqian; Yang, Ru; Wang, Shunli; Zhang, Xiuxin

doi:10.1016/s2095-3119(18)62131-8

Cited by 41 publications

(34 citation statements)

References 48 publications

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“…PsGID1 is upstream of PsGAI , and more PsGID1 expression will repress PsGAI expression [13]. The expression results of the two GA signaling genes were similar with the previous study [13]. Endogenous GA 3 could promote exogenous GA biosynthesis with 4 h treatment, and more biosynthetic GA induced PsGID1 expression and repressed PsGAI expression (Fig.…”

Section: Resultssupporting

confidence: 81%

“…PsGAI and PsGID1 are two important GA signaling genes. PsGID1 is upstream of PsGAI , and more PsGID1 expression will repress PsGAI expression [13]. The expression results of the two GA signaling genes were similar with the previous study [13].…”

Section: Resultssupporting

confidence: 78%

“…The flowering of tree peonies is a response to cues related to light, temperature, and other external influences [1, 2, 12, 13, 25]. Although the analysis of DEGs in re-blooming and non-re-blooming cultivars, or early and late flowering period cultivars has identified some important flowering time genes and re-blooming genes using Illumina HiSeq™ 2000 and Illumina HiSeq™ 2500 platforms [15, 16, 23], the flowering induction pathway in tree peony remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The expression levels of DEGs, such as GAI and GID1 , in the GA pathway showed significant changes (Additional file 5: Table S2). Combining the results of effects of endogenous GA 3 on flowering quality of ‘Luoyang Hong’ and the re-blooming mechanism of ‘High Noon’, it was deduced that the GA pathway and vernalization pathway were also important pathways in tree peony [13, 16]. PdSPL9 played important roles in the juvenile-to-adult phase transition, suggesting that the age pathway was also important in tree peony [12].…”

Section: Discussionmentioning

confidence: 99%

“…These studies focused on cultivar selection, physiological change, chilling effect, and hormone analysis [1, 2, 10–12]. The effects of exogenous GA 3 on flowering quality, endogenous hormones, and hormone- and flowering-associated gene expression in a forcing culture of tree peony were also deciphered [13]. Endo-dormancy-imposed growth arrest is one of the key characteristics preventing tree peony from flowering well.…”

Section: Introductionmentioning

confidence: 99%

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De novo sequencing of tree peony (Paeonia suffruticosa) transcriptome to identify critical genes involved in flowering and floral organ development

et al. 2019

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Background Tree peony ( Paeonia suffruticosa Andrews) is a globally famous ornamental flower, with large and colorful flowers and abundant flower types. However, a relatively short and uniform flowering period hinders the applications and production of ornamental tree peony. Unfortunately, the molecular mechanism of regulating flowering time and floral organ development in tree peony has yet to be elucidated. Because of the absence of genomic information, 454-based transcriptome sequence technology for de novo transcriptomics was used to identify the critical flowering genes using re-blooming, non-re-blooming, and wild species of tree peonies. Results A total of 29,275 unigenes were obtained from the bud transcriptome, with an N50 of 776 bp. The average length of unigenes was 677.18 bp, and the longest sequence was 5815 bp. Functional annotation showed that 22,823, 17,321, 13,312, 20,041, and 9940 unigenes were annotated by NCBI-NR, Swiss-Prot, COG, GO, and KEGG, respectively. Within the differentially expressed genes (DEGs) 64 flowering-related genes were identified and some important flowering genes were also characterized by bioinformatics methods, reverse transcript polymerase chain reaction (RT-PCR), and rapid-amplification of cDNA ends (RACE). Then, the putative genetic network of flowering induction pathways and a floral organ development model were put forward, according to the comparisons of DEGs in any two samples and expression levels of the important flowering genes in differentiated buds, buds from different developmental stages, and with GA or vernalization treated. In tree peony, five pathways (long day, vernalization, autonomous, age, and gibberellin) regulated flowering, and the floral organ development followed an ABCE model. Moreover, it was also found that the genes PsAP1 , PsCOL1 , PsCRY1 , PsCRY2 , PsFT , PsLFY , PsLHY , PsGI , PsSOC1 , and PsVIN3 probably regulated re-blooming of tree peony. Conclusion This study provides a comprehensive report on the flowering-related genes in tree peony for the first time and investigated the expression levels of the critical flowering related genes in buds of different cultivars, developmental stages, differentiated primordium, and flower parts. These results could provide valuable insights into the molecular mechanisms of flowering time regulation and floral organ development. Electronic supplementary material The online version of this article (10.1186/s12864-019-5857-0) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 81%

Section: Resultssupporting

confidence: 78%