Effect of exogenous hormones on transcription levels of pyridoxal 5′-phosphate biosynthetic enzymes in the silkworm (Bombyx mori)

Huang, ShuoHao; Yang, Huanhuan; Yao, Lili; Zhang, Jianyun; Huang, LongQuan

doi:10.1016/j.cbpb.2016.01.002

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…In Bombyx mori caterpillars, PLP levels are discussed to be critical for progression of the different larval stages, and recent research indicates that expression of PDXH and PDXK genes is under the control of the juvenile and molting hormones [ 65 ].…”

Section: Regulation Of Salvage Pathway Genesmentioning

confidence: 99%

Vitamin B6 and Its Role in Cell Metabolism and Physiology

Parra

Stahl

Hellmann

2018

Cells

316

159

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Vitamin B6 is one of the most central molecules in cells of living organisms. It is a critical co-factor for a diverse range of biochemical reactions that regulate basic cellular metabolism, which impact overall physiology. In the last several years, major progress has been accomplished on various aspects of vitamin B6 biology. Consequently, this review goes beyond the classical role of vitamin B6 as a cofactor to highlight new structural and regulatory information that further defines how the vitamin is synthesized and controlled in the cell. We also discuss broader applications of the vitamin related to human health, pathogen resistance, and abiotic stress tolerance. Overall, the information assembled shall provide helpful insight on top of what is currently known about the vitamin, along with addressing currently open questions in the field to highlight possible approaches vitamin B6 research may take in the future.

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Section: Regulation Of Salvage Pathway Genesmentioning

confidence: 99%

Vitamin B6 and Its Role in Cell Metabolism and Physiology

Parra

Stahl

Hellmann

2018

Cells

316

159

View full text Add to dashboard Cite

show abstract

“…Ultimately, the cocoon yield decreased in silkworms reared on formula feed. Moreover, pyridoxine 5′-phosphate can be converted into pyridoxal 5′-phosphate through interaction with the pyridoxine-5′-phosphate oxidase ( Huang et al, 2016a ; Huang et al, 2016b ). Pyridoxine-5′-phosphate oxidase is regulated by development and hormones (juvenile hormone, molting hormone) in silkworms ( Huang et al, 2016a ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, pyridoxine 5′-phosphate can be converted into pyridoxal 5′-phosphate through interaction with the pyridoxine-5′-phosphate oxidase ( Huang et al, 2016a ; Huang et al, 2016b ). Pyridoxine-5′-phosphate oxidase is regulated by development and hormones (juvenile hormone, molting hormone) in silkworms ( Huang et al, 2016a ). Thus, the lower pyridoxine 5′-phosphate in the FF silkworms might be related to the hormone levels-a possibility requiring further validation.…”

Section: Discussionmentioning

confidence: 99%

Multi-tissue metabolomic profiling reveals potential mechanisms of cocoon yield in silkworms (Bombyx mori) fed formula feed versus mulberry leaves

Chen

et al. 2022

Front. Mol. Biosci.

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Use of formula feed (FF) for silkworms for all instars, has promoted transformation and progress in traditional sericulture. However, the cocoon yield of FF silkworms has failed to reach that of silkworms fed mulberry leaves (ML). The biological mechanisms underlying this phenomenon have not been well described. This study aimed to identify metabolic mechanisms and potential biomarkers relating to the poor cocoon yield of FF silkworms. In this study, silkworms received treatments of either ML (ML group) or FF (FF group) for all instars. At the 3rd day of the 5th instar, the midgut (MG), hemolymph (HL) and posterior silk gland (PSG) were collected for the metabolome profiles detection. The remaining silkworms were fed ML or FF until cocooning for investigation. The whole cocoon yield (WCY) was significantly higher in the FF group than the ML group (p < 0.05), whereas the cocoon shell weight (CSW) and cocoon shell rate (CSR) were significantly lower in the FF group (p < 0.05). A total of 845, 867 and 831 metabolites were qualified and quantified in the MG, HL and PSG of the FF silkworms, respectively. Correspondingly, 789, 833 and 730 metabolites were quantified in above three tissues of the ML group. Further, 230, 249 and 304 significantly different metabolites (SDMs) were identified in the MG, HL and PSG between the FF and ML group, respectively. Eleven metabolic pathways enriched by the SDMs were mutual among the three tissues. Among them, cysteine and methionine metabolism, arginine biosynthesis, and arginine and proline metabolism were the top three pathways with the highest impact value in the PSG. Six biomarkers were obtained through biomarker analysis and Pearson correlation calculation. Among them, homocitrulline, glycitein, valyl-threonine, propyl gallate and 3-amino-2,3-dihydrobenzoic acid were positively correlated with WCY, but negatively correlated with CSW and CSR (p < 0.05). An opposite correlation pattern was observed between 3-dimethylallyl-4-hydroxyphenylpyruvate and the three cocoon performance traits. Overall, three key metabolic pathways and six biomarkers associated with cocoon yield were interpreted, and should provide directions for formula feed optimization in factory-raised silkworms.

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“…In silkworm larvae, super uous amino acids are deaminized in the fat body and midgut, thus producing ammonia, which is removed in three forms [29]. Part of the ammonia is excreted in the form of ammonium salt by the Malpighian tubules [29], and a portion of the ammonia is stored as amides to provide amidogen for amino acid synthesis [30], or is involved in purine synthesis and eventually metabolized to uric acid or urea [31,32]. Uric acid and urea, the end-product of amino acid metabolism, were signi cantly higher in all three tissues in the FF group than the ML group, possibly because of the higher urease activity of mulberry leaves [33].…”

Section: Signi Cantly Different Metabolitesmentioning

confidence: 99%

Multi-tissue metabolomic profiling reveals potential biomarkers of cocoon yield in silkworms (Bombyx mori) fed formula feed versus mulberry leaves

Chen

et al. 2022

Preprint

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Background Use of formula feed (FF) for silkworms for all instars, has promoted transformation and progress in traditional sericulture. However, the cocoon yield of FF silkworms has failed to reach that of silkworms fed mulberry leaves (ML). The biological mechanisms underlying this phenomenon have not been well described. This study aimed to identify potential biomarkers and metabolic mechanisms relating to the poor cocoon yield of FF silkworms. In this study, silkworms received treatments of either ML (ML group) or FF (FF group) for all instars. At the 3rd day of the 5th instar, the hemolymph, midgut and posterior silk gland were collected for the metabolome profiles detection. The remaining silkworms were fed ML or FF until cocooning for investigation. Results The whole cocoon yield (WCY) was significantly higher in the FF group than the ML group (P < 0.05), whereas the cocoon shell weight (CSW) and cocoon shell rate (CSR) were significantly lower in the FF group (P < 0.05). A total of 867, 845 and 831 metabolites were qualified and quantified in the hemolymph, midgut and posterior silk gland of the FF silkworms, respectively. Correspondingly, 833, 789 and 730 metabolites were quantified in above three tissues of the ML group. Further, 249, 230 and 304 significantly different metabolites (SDMs) were identified in the hemolymph, midgut and posterior silk gland between the FF and ML group, respectively. Eleven metabolic pathways enriched by the SDMs were mutual among the three tissues. Among them, cysteine and methionine metabolism, arginine biosynthesis, and arginine and proline metabolism were the top three pathways with the highest impact value in the posterior silk gland. Six biomarkers were obtained through biomarker analysis and Pearson correlation calculation. Among them, homocitrulline, glycitein, valyl-threonine, propyl gallate and 3-amino-2,3-dihydrobenzoic acid were positively correlated with WCY, but negatively correlated with CSW and CSR (P < 0.05). An opposite correlation pattern was observed between 3-dimethylallyl-4-hydroxyphenylpyruvate and the three cocoon performance traits. Conclusions Overall, three key metabolic pathways and six biomarkers associated with cocoon yield were interpreted, and should provide directions for formula feed optimization in factory-raised silkworms.

show abstract

Effect of exogenous hormones on transcription levels of pyridoxal 5′-phosphate biosynthetic enzymes in the silkworm (Bombyx mori)

Cited by 7 publications

References 34 publications

Vitamin B6 and Its Role in Cell Metabolism and Physiology

Vitamin B6 and Its Role in Cell Metabolism and Physiology

Multi-tissue metabolomic profiling reveals potential mechanisms of cocoon yield in silkworms (Bombyx mori) fed formula feed versus mulberry leaves

Multi-tissue metabolomic profiling reveals potential biomarkers of cocoon yield in silkworms (Bombyx mori) fed formula feed versus mulberry leaves

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