The purpose of the present study is to recover marble waste and expanded perlite aggregate (EPA) for use as an additive to cementitious matrix building materials. The main goal is to produce a new insulation block floor from lightweight concrete (LC) by mixing sand from the waste marble crushing process (SWM), natural sand, and EPA. First, optimal mixture of natural sand, SWM, and EPA was determined for a given insulation LC. To this end, plate and cubic specimens were prepared by varying the volume proportion of SWM to natural sand in percentages of 0, 20, 40, 60, 80, and 100. Mechanical and physical properties such as the compressive strength, thermal conductivity, thermal diffusivity, specific heat capacity, and sound reduction index at different frequencies were investigated. Finally, a prototype of a new insulation lightweight block floor was manufactured from the optimal mixture of the studied LC. The results showed that the incorporation of SWM significantly improved the mechanical properties and the thermal insulation of LC compared to those of the natural sand. These results are promising and give the present insulation block floor the opportunity to be used in composite slabs.