Effect of experimental arginine supplementation on the growth, immunity and resistance of tilapia fingerlings to Streptococcus agalactiae

Abstract

Streptococcosis causes serious economic losses to fish farms every year. A four-week feeding trial was carried out to evaluate arginine (ARG) supplementation in the diet of Nile tilapia (Oreochromis niloticus) fingerlings challenged by Streptococcus agalactiae.Fish were fed with experimental diets containing five levels of supplementation with 1.39%, 1.76%, 1.97%, 2.18% or 2.39% analysed level of ARG. Each diet was randomly distributed in 30 tanks containing 20 fish/tank. After 30 days, no differences were observed in performance parameters, weight gain, daily weight gain, specific growth rate, feed intake or food conversion. After the performance assay, fingerlings were infected with 1.95 × 10 8 CFU/fish of Streptococcus agalactiae. Mortality was verified daily, and the respiratory burst of leucocytes and nitric oxide production were measured at 0 hr, 24 hr, 7 days and 15 days after infection. The survival rate of the fish is compared using the Kaplan-Meier method, with values of 4.29%, 14.8%, 10.8%, 17.2% and 33.3% for the 1.39, 1.76, 1.97, 2.18 and 2.39 treatments respectively. A reduction in the mortality rate and a boost in the immune responses was observed in all the supplementations of ARG in the diet; however, the best survival rates were obtained in the treatments with 2.39% ARG as well as the most efficient immune responses.

Dietary arginine requirement in diets of GIFT strain of Nile tilapia, Oreochromis niloticus : Effects on growth performance, whole‐body composition, growth‐related gene expression and haemato‐biochemical responses

“…Haematological and biochemical evaluation of blood is important in assessing fish health with respect to dietary manipulation TA B L E 6 Biochemical and haematological parameters of GIFT tilapia juveniles fed graded levels of L-arginine (Metochis et al, 2017). Dietary arginine supplementation plays central role in the modulation of immune responses (Vianna et al, 2020). Arginine in the diet facilitates positive effects on both haematological and innate immune responses, such as haematocrit, haemoglobin, phagocytosis and circulating erythrocytes (Buentello et al, 2007).…”

Effect of Fluorofenidone Against Paraquat-Induced Pulmonary Fibrosis Based on Metabolomics and Network Pharmacology

“…In this study, we found dysregulation in arginine, tryptophan, and L-kynurenine. Arginine can affect cellular immunity under stress conditions [ 43 ]. Tryptophan is essential for protein synthesis and some physiological metabolic functions [ 44 ].…”

Arginine metabolism and its functions in growth, nutrient utilization, and immunonutrition of fish

“…Dietary arginine supplementation from 2.10% to 3.82% also significantly enhanced the innate immune responses, and decreased the cumulative mortality rate of juvenile turbot infected with E. tarda ( Zhang et al., 2017 ). A reduction in the mortality rate of Nile tilapia fingerlings after Streptococcus agalactiae challenge and a boost in immune responses were observed in all supplementations of arginine (from 1.55% to 2.39%) in the diet ( Vianna et al., 2020 ). Dietary arginine supplementation (from 2.08% to 3.48%) also modulated nonspecific immune responses in Indian major carp ( Cirrhinus mrigala ) exposed to hypoxia and reduced its mortality after A. hydrophila infection ( Varghese et al., 2020 ).…”