Effect of exposure conditions on SWCNT-induced inflammatory response in human alveolar epithelial cells

Baktur, Rena; Patel, Hemang; Kwon, Soonjo

doi:10.1016/j.tiv.2011.04.001

Cited by 28 publications

(10 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro studies have also demonstrated that NPs can up-regulate IL-8 in human lung epithelial cells. Baktur et al demonstrated that IL-8 expression is enhanced in the presence of serum when carbon nanotubes (SWCNTs) were exposed to A549 cells even if a lower cytotoxicity was observed (Baktur et al 2011). This is because more SWCNTs penetrated into cells in the presence of serum.…”

Section: Discussionmentioning

confidence: 99%

Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles

Hsiao

Huang

2013

J Nanopart Res

View full text Add to dashboard Cite

Although an increasing number of in vitro studies are being published regarding the cytotoxicity of nanomaterials, the components of the media for toxicity assays have often varied according to the needs of the scientists. Our aim for this study was to evaluate the influence of serum—in this case, fetal bovine serum—in a cell culture medium on the toxicity of nano-sized (50–70 nm) and micro-sized (<1 μm) ZnO on human lung epithelial cells (A549). The nano- and micro-sized ZnO both exhibited their highest toxicity when exposed to serum-free media, in contrast to exposure in media containing 5 or 10 % serum. This mainly comes not only from the fact that ZnO particles in the serum-free media have a higher dosage-per-cell ratio, which results from large aggregates of particles, rapid sedimentation, absence of protein protection, and lower cell growth rate, but also that extracellular Zn2+ release contributes to cytotoxicity. Although more extracellular Zn2+ release was observed in serum-containing media, it did not contribute to nano-ZnO cytotoxicity. Furthermore, non-dissolved particles underwent size-dependent particle agglomeration, resulting in size-dependent toxicity in both serum-containing and serum-free media. A low correlation between cytotoxicity and inflammation endpoints in the serum-free medium suggested that some signaling pathways were changed or induced. Since cell growth, transcription behavior for protein production, and physicochemical properties of ZnO particles all were altered in serum-free media, we recommend the use of a serum-containing medium when evaluating the cytotoxicity of NPs.Electronic supplementary materialThe online version of this article (doi:10.1007/s11051-013-1829-5) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles

Hsiao

Huang

2013

J Nanopart Res

View full text Add to dashboard Cite

show abstract

“…Previous investigations have shown that exposure of human primary monocytes to carboxylated SWCNTs and exposure of A549 cells to MWCNTs led to IL-6 and IL-8 expression and the inflammatory response in these cells were through oxidative stress and NF-κB activation (Ye et al, 2011;Ye et al, 2009). Our results suggested that pristine SWCNTs might interact with the cellular system in a similar way as carboxylated SWCNTs and MWCNTs and also induce inflammatory response through NF-κB activation (Baktur et al, 2011). Profilin, a ubiquitous actin monomer-binding protein, is thought to regulate actin polymerization in response to extra cellular signals (Mahoney et al, 1997) and its up-regulation might prevent the polymerization of actin when cells were exposed to SWCNTs.…”

Section: Protein Profile Obtained From Itraq Based 2d Lc-ms/ms Analysissupporting

confidence: 65%

“…Due to these properties, graphene and CNTs are considered to be promising materials in high-energy physics (Novoselov et al, 2005;Zhang et al, 2005), material science (Akhavan, 2010;Bai et al, 2010;Hu et al, 2010;Shang et al, 2008;Wu et al, 2009a), and a wide range of technological applications, such as biosensor, bio-imaging, tissue engineering and drug delivery (Choi et al, 2010;Liu et al, 2008a;Liu et al, 2009b;Sun et al, 2008b;Veetil and Ye, 2009). Previous investigations have demonstrated that single-walled carbon nanotubes (SWCNTs) induce a noticeable cytotoxicity to mammalian cells (Baktur et al, 2011;Manna et al, 2005;Raja et al, 2007;Shvedova et al, 2003;Wang et al, 2011;Zeni et al, 2008). Among the suggested toxicity mechanisms such as oxidative stress, rupture of cell membrane and interfering intracellular metabolic routes, the oxidative stress has been considered to be the most acceptable mechanism explaining the cytotoxicity of CNTs.…”

Section: Introductionmentioning

confidence: 99%

Proteomics study of the cellular function in response to nanomaterials

Yuan

View full text Add to dashboard Cite

“…THP-1 cells were employed to represent the resident phagocytic cells that have the main function of removing pathogens, senescent cells and external particles from the lungs2930, while A549 cells represented an in vitro model for lung alveoli cells, which are not associated with inflammatory responses. A549 cells have been extensively used for assessing pulmonary cytotoxicity, including nanomaterials-induced cytotoxicity103132.…”

Section: Discussionmentioning

confidence: 99%

Citrullination as early-stage indicator of cell response to Single-Walled Carbon Nanotubes

Mohamed

Movia

Knyazev

et al. 2013

Sci Rep

View full text Add to dashboard Cite

Single-walled carbon nanotubes (SWCNTs) have been widely explored as potential technologies for information systems and medical applications. The impact of SWCNTs on human health is of prime concern, if SWCNTs have a future in the manufacturing industry. This study proposes a novel, inflammation-independent paradigm of toxicity for SWCNTs, identifying the protein citrullination process as early-stage indicator of inflammatory responses of macrophages (THP-1) and of subtle phenotypic damages of lung epithelial (A549) cells following exposure to chemically-treated SWCNTs. Our results showed that, while most of the cellular responses of A549 cells exposed to SWCNTs are different to those of similarly treated THP-1 cells, the protein citrullination process is triggered in a dose- and time-dependent manner in both cell lines, with thresholds comparable between inflammatory (THP-1) and non-inflammatory (A549) cell types. The cellular mechanism proposed herein could have a high impact in predicting the current risk associated with environmental exposure to SWCNTs.

show abstract

Effect of exposure conditions on SWCNT-induced inflammatory response in human alveolar epithelial cells

Cited by 28 publications

References 51 publications

Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles

Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles

Proteomics study of the cellular function in response to nanomaterials

Citrullination as early-stage indicator of cell response to Single-Walled Carbon Nanotubes

Contact Info

Product

Resources

About