Effect of exposure to fluoroquinolones and beta‐lactams on the <i>in vitro</i> activity of other groups of antibiotics in <i>Salmonella spp.</i>

Cebrián, Laura; Rodrı́guez, Juan Carlos; Escribano, Isabel; Royo, Gloria

doi:10.1111/j.1600-0463.2006.apm_357.x

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…This subcultured strain is considered to be resistant to amoxicillin (MIC > 8 μ g/mL) according to EUCAST's recommendations [ 22 ]. These results corroborate with those obtained by Cebrian et al [ 23 ] who showed that the in vitro exposure of Salmonella strains to amoxicillin induced a reduction in sensitivity to amoxicillin and other antibiotics. Furthermore, Qureshi et al [ 24 ] reported similar results for Helicobacter pylori strains.…”

Section: Discussionsupporting

confidence: 92%

“…is subcultured strain is considered to be resistant to amoxicillin (MIC > 8 μg/mL) according to EUCAST's recommendations [22]. ese results corroborate with those obtained by Cebrian et al [23] who showed that the in vitro exposure of Salmonella strains to amoxicillin Our results also show that E. coli strain became less sensitive to tylosin and colistin, while these two antibiotics were not added to the subculture medium. e MICs of tylosin and colistin increased by 4-fold compared to the starting MICs.…”

Section: Discussionsupporting

confidence: 89%

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In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

Hriouech

Akhmouch

Tanghort

et al. 2020

Veterinary Medicine International

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This study aims at verifying, in vitro, the extent to which the use of amoxicillin or thymol induces the selection of resistant bacteria and at evaluating in vivo their effects on the development of antimicrobial resistance in the intestinal flora of poultry. E. coli strain was subcultured on agar plates containing increasing concentrations of either amoxicillin or thymol. Thereafter, minimal inhibitory concentrations (MICs) of thymol, amoxicillin, and two other antibiotics, tylosin and colistin, were determined using the microdilution method. Groups of chicks were subjected to a 2-week regime of either amoxicillin or thymol added to their drinking water. During the treatment with either thymol or amoxicillin, the total aerobic mesophilic flora (TAMF) was counted on thymol-gradient plates or amoxicillin-gradient plates and the MICs of antibiotics and thymol for E. coli isolates were determined. The in vitro test showed that for E. coli, which had been serially subcultured on increasing concentrations of amoxicillin, a 32-fold increase in MIC values for amoxicillin and a 4-fold increase for colistin and tylosin were noted. However, the MIC of thymol for this strain remained constant. For the E. coli, which had been serially subcultured on increasing concentrations of thymol, no change in the MIC values for antibiotics and thymol was observed. The in vivo test confirmed the in vitro one. It demonstrated that exposure to amoxicillin induced a selection of antimicrobial resistance in TAMF and intestinal E. coli, whereas exposure to thymol did not. The results showed that the group receiving thymol had a lower consumption index compared to the other groups. This study demonstrates the feasibility of this natural product as an alternative solution to the current use of antibiotics in poultry farming.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

Hriouech

Akhmouch

Tanghort

et al. 2020

Veterinary Medicine International

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“…They found that mutants generated after treatments with various FQs showed reduced susceptibility to a wide range of the antibiotics tested (seven different antibiotics). This was in contrast to mutants generated under treatment with β-lactams, which showed reduced susceptibility to fewer antibiotics (five antibiotics) [179]. Similarly, it has been shown that the treatment of methicillin-resistant St. aureus with sublethal FQ further enhances methicillin resistance [169].…”

Section: Treatment With Quinolones Increases Resistance To Non-quinolmentioning

confidence: 89%

Quinolones: Mechanism, Lethality and Their Contributions to Antibiotic Resistance

et al. 2020

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Fluoroquinolones (FQs) are arguably among the most successful antibiotics of recent times. They have enjoyed over 30 years of clinical usage and become essential tools in the armoury of clinical treatments. FQs target the bacterial enzymes DNA gyrase and DNA topoisomerase IV, where they stabilise a covalent enzyme-DNA complex in which the DNA is cleaved in both strands. This leads to cell death and turns out to be a very effective way of killing bacteria. However, resistance to FQs is increasingly problematic, and alternative compounds are urgently needed. Here, we review the mechanisms of action of FQs and discuss the potential pathways leading to cell death. We also discuss quinolone resistance and how quinolone treatment can lead to resistance to non-quinolone antibiotics.

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“…Previously, we have studied the different capacity of antibiotics to generate fluoquinolone‐resistant mutants in clinical isolates of various Salmonella spp. serotypes (2, 3) and the influence of previous exposition to fluoroquinolones and beta‐lactams on the in vitro activity of other groups of antibiotics (4). In this study, we try to characterize the subpopulations most resistant to fluoroquinolones, produced following repeated exposure to subinhibitory concentrations of different fluoroquinolones and beta‐lactams, by determining the mutant prevention concentration.…”

mentioning

confidence: 99%

Evaluation of several fluoroquinolones and beta‐lactams in terms of their capability to restrict the selection of fluoroquinolone‐resistant Salmonella: in vitro models

Cebrián

Rodríguez

Escribano³

et al. 2007

APMIS

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With a view to understanding the interaction between Salmonella and the drugs used to treat it, our aim was to compare the different capacities of various antibiotics to generate mutants resistant to fluoroquinolones following repeated exposure of the microorganisms to subinhibitory concentrations of these drugs. Mutants were generated by repeated exposure to fluoroquinolones and beta-lactams. In order to compare the different capacity to generate resistant mutants, we studied the minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of the wild-type strains and of the mutants generated. These data were compared with pharmacokinetic parameters. Mutants generated following repeated exposure to fluoroquinolones exhibit an increased MPC as compared to the wild-type strains, both in strains that are nalidixic acid susceptible and in those that are nalidixic acid resistant, with repeated exposure to ciprofloxacin leading to the smallest increases. This increase in MPC is gradual and depends on the number of exposures the bacteria are subjected to. It results in a decrease in the AUC/MPC ratio, although the absolute values vary. Ciprofloxacin is the most active drug, both against nalidixic acid-susceptible and nalidixic acid-resistant strains, although in late mutants of originally nalidixic acid-resistant strains, the AUC/MPC values are low. Repeated exposure to amoxicillin and cefotaxime also produces an increase in the MPC of fluoroquinolones, with ciprofloxacin being the least affected. Exposure to amoxicillin leads to the greatest increase in the MPC of fluoroquinolones. When the AUC/MPC ratios of these mutants are compared, the values are still seen to be high (between 25 and 75). When we compare the MPC data with the antibiotic levels in humans following administration of the usual doses, it can be seen that ciprofloxacin exhibits the highest AUC/MPC and therefore the lowest risk of therapeutic failures. In addition, administration of subinhibitory concentrations of beta-lactams produces a decrease in fluoroquinolone susceptibility, which may lead to an increase in the risk of therapeutic failure if these compounds are subsequently used.

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Effect of exposure to fluoroquinolones and beta‐lactams on the in vitro activity of other groups of antibiotics in Salmonella spp.

Cited by 6 publications

References 29 publications

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

Quinolones: Mechanism, Lethality and Their Contributions to Antibiotic Resistance

Evaluation of several fluoroquinolones and beta‐lactams in terms of their capability to restrict the selection of fluoroquinolone‐resistant Salmonella: in vitro models

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