Effect of Extraction and Drying Method on Chemical Composition, and Evaluation of Antioxidant and Antimicrobial Activities of Essential Oils from <i>Salvia officinalis</i> L.

Boutebouhart, Hadjer; Didaoui, Linda; Tata, S. J.; Sabaou, Nasserdine

doi:10.1080/0972060x.2019.1651223

Cited by 18 publications

(19 citation statements)

References 27 publications

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“…Nonetheless, the essential oil compositions and yields obtained from S. officinalis have been extensively studied [ 7 , 16 , 17 , 18 , 19 ], and the effects of the extraction procedures, drying methods, and harvesting frequency on the plant’s essential oil constituents have been demonstrated in previous reports [ 14 , 15 , 20 , 21 ]. The current work instead, in contrast to the previous reports on the essential oils of sage from different locations, seasons, and multiple drying methods, dealt with the periodic effects of a single drying procedure that is frequently adopted by the apothecary and herbal dealers, i.e., room temperature and natural atmospheric pressure conditions for drying the herbs.…”

Section: Introductionmentioning

confidence: 99%

Sage, Salvia officinalis L., Constituents, Hepatoprotective Activity, and Cytotoxicity Evaluations of the Essential Oils Obtained from Fresh and Differently Timed Dried Herbs: A Comparative Analysis

et al. 2021

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Sage, Salvia officinalis L., is used worldwide as an aromatic herb for culinary purposes as well as a traditional medicinal agent for various ailments. Current investigations exhibited the effects of extended dryings of the herb on the yields, composition, oil quality, and hepatoprotective as well as anti-cancer biological activities of the hydrodistillation-obtained essential oils from the aerial parts of the plant. The essential oils’ yields, compositions, and biological activities levels of the fresh and differently timed and room-temperature dried herbs differed significantly. The lowest yields of the essential oil were obtained from the fresh herbs (FH, 631 mg, 0.16%), while the highest yield was obtained from the two-week dried herbs (2WDH, 1102 mg, 0.28%). A notable decrease in monoterpenes, with increment in the sesquiterpene constituents, was observed for the FH-based essential oil as compared to all the other batches of the essential oils obtained from the different-timed dried herbs. Additionally, characteristic chemotypic constituents of sage, i.e., α-pinene, camphene, β-pinene, myrcene, 1, 8-cineole, α-thujone, and camphor, were present in significantly higher proportions in all the dried herbs’ essential oils as compared to the FH-based essential oil. The in vivo hepatoprotective activity demonstrated significant reductions in the levels of AST, ALT, and ALP, as well as a significant increase in the total protein (p < 0.05) contents level, as compared to the acetaminophen (AAP) administered experimental group of rats. A significant reduction (p < 0.05) in the ALT level was demonstrated by the 4WDH-based essential oil in comparison to the FH-based essential oil. The levels of creatinine, cholesterol, and triglycerides were reduced (p < 0.05) in the pre-treated rats by the essential oil batches, with non-significant differences found among them as a result of the herbs dryings based oils. A notable increase in the viability of the cells, and total antioxidant capacity (TAOxC) levels, together with the reduction in malondialdehyde (MDA) levels were observed by the essential oils obtained from all the batches as compared with the AAP-treated cell-lines, HepG-2, HeLa, and MCF-7, that indicated the in vitro hepatoprotective effects of the sage essential oils. However, significant improvements in the in vivo and in vitro hepatoprotective activities with the 4WDH-based oil, as compared to all other essential oil-batches and silymarin standard demonstrated the beneficial effects of the drying protocol for the herb for its medicinal purposes.

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Section: Introductionmentioning

confidence: 99%

Sage, Salvia officinalis L., Constituents, Hepatoprotective Activity, and Cytotoxicity Evaluations of the Essential Oils Obtained from Fresh and Differently Timed Dried Herbs: A Comparative Analysis

et al. 2021

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“…It is a small, evergreen perennial plant originated from the Mediterranean region, which has been found to have not only culinary, but also health-promoting applications. Most of the papers regarding this plant are aimed at qualitative and quantitative evaluation of the components of the essential oil extracted from sage leaves [ 1 , 2 , 3 ]. In traditional medicine, Salvia is used to treat various disorders, including epilepsy, gout, inflammation, as well as diarrhea and hyperglycemia [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves

Francik

Sadowska

et al. 2020

Materials

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The influence of harvest period and drying method of Salvia officinalis L. leaves on the content of essential oils, polyphenols and antioxidant properties was investigated. Sage leaves were collected twice during plant blossoming (in June and July) and dried traditionally in natural conditions and at 35 °C. Antioxidant activity was assayed in methanol-acetone extracts and infusions of dried leaves with the use of free radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP) technique. Total phenolic content in extracts as well as in infusions was determined by the means of Folin–Ciocalteu method. Based on the LC/MS analysis, the polyphenol compounds present in both extracts and in infusions were identified. The extracts contained more polyphenols and were characterized by higher antioxidant activity than infusions. In the extracts significant amount of ferulic acid was found, which was influenced both by the harvest period and drying method. The amount of ferulic acid found in extracts obtained from the June leaves dried traditionally was three times lower (6.185 μg/g DW) than in extracts from July leaves dried in the same conditions. Harvest period had a significant effect on the essential oils’ content, leaves collected in July contained 15% more oils than those collected in June.

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“…Interestingly, Boutebouhart et al [ 55 ] compared the antioxidant activity of S. officinalis leaves EO cultivated in Algeria and obtained by microwave-assisted hydrodistillation, conventional hydrodistillation technique, and steam distillation. The EOs obtained by steam distillation and hydrodistillation presented the highest percentage of DPPH radical inhibition with values of 40.25% and 36.75%, respectively, (at 1000 mg/L).…”

Section: Resultsmentioning

confidence: 99%

Salvia officinalis L. from Italy: A Comparative Chemical and Biological Study of Its Essential Oil in the Mediterranean Context

et al. 2020

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Salvia officinalis L. (sage) is one of the most appreciated plants for its plethora of biologically active compounds. The objective of our research was a comparative study, in the Mediterranean context, of chemical composition, anticholinesterases, and antioxidant properties of essential oils (EOs) from sage collected in three areas (S1–S3) of Southern Italy. EOs were extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory properties were investigated by employing Ellman’s method. Four in vitro assays, namely, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric-reducing ability power (FRAP), and β-carotene bleaching tests, were used to study the antioxidant effects. Camphor (16.16–18.92%), 1,8-cineole (8.80–9.86%), β-pinene (3.08–9.14%), camphene (6.27–8.08%), and α-thujone (1.17–9.26%) are identified as the most abundant constituents. However, the content of these constituents varied depending on environmental factors and pedoclimatic conditions. Principal component analysis (PCA) was performed. Based on Relative Antioxidant Capacity Index (RACI), S2 essential oil exhibited the highest radical potential with an IC50 value of 20.64 μg/mL in ABTS test and presented the highest protection of lipid peroxidation with IC50 values of 38.06 and 46.32 μg/mL after 30 and 60 min of incubation, respectively. The most promising inhibitory activity against BChE was found for S3 sample (IC50 of 33.13 μg/mL).

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Effect of Extraction and Drying Method on Chemical Composition, and Evaluation of Antioxidant and Antimicrobial Activities of Essential Oils from Salvia officinalis L.

Cited by 18 publications

References 27 publications

Sage, Salvia officinalis L., Constituents, Hepatoprotective Activity, and Cytotoxicity Evaluations of the Essential Oils Obtained from Fresh and Differently Timed Dried Herbs: A Comparative Analysis

Sage, Salvia officinalis L., Constituents, Hepatoprotective Activity, and Cytotoxicity Evaluations of the Essential Oils Obtained from Fresh and Differently Timed Dried Herbs: A Comparative Analysis

Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves

Salvia officinalis L. from Italy: A Comparative Chemical and Biological Study of Its Essential Oil in the Mediterranean Context

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