Effect of Extraction Procedure on Yield, Phytochemical Profile and Antibacterial Activity of Fenugreek Seed (Trigonella foenum-graecum) Crude Extracts

Abstract

Background: Fenugreek (Trigonella foenum-graecum) is a medicinal plant known for its various pharmacological properties, including its antibacterial activity. The purpose of this study was to assess the extraction procedure effect on yield, phytochemical profile and antibacterial activity of fenugreek seed crude extracts. Methods: The extraction procedure of components from fenugreek seed possessing an antibacterial potential was first optimized. Extracts at the highest yield were analyzed for phytocomponents and their bacterial growth inhibitory effect was assessed by determining the diameter of the inhibition zone (IZ) and the minimum inhibition concentration (MIC). Results: Optimum conditions for highest yield of extract (270,78 mg/g) are as follows: solvent (water), substrate-solvent ratio (1:10), and extraction time (72 hours). Phytochemical screening revealed the presence of the major compounds known to have an antibacterial activity such as tannins and flavonoids in the aqueous and methanol extracts. The methanol extract was associated with an IZ diameter ranging from 8.46±2.25 mm up to 27.65±1.32 mm and displayed the lowest MIC (1.25 mg/ml). Our findings indicate that fenugreek seed extracted with methanol showed the best antibacterial effect against the targeted bacterial strain.

Anti‐shigellosis activity of the aqueous extract of garlic, clove and fenugreek

“…Several studies have shown that Fenugreek has in-vitro antibacterial potential against Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumonia, and Shigella sonnei. This activity is attributed to the plant's high concentration of phenolic and flavonoid compounds (Chalghoumi, Mabrouki, Abdouli, & Line, 2020). On the other hand, the MIC of Nigella sativa seed volatile oil for Shigella, Vibrio, and Escherichia strains has been reported to be between 50 and 400 μg/ml (Ferdous, Islam, Ahsan, Hasan, & Ahmed, 1992).…”

Phytochemical Screening and Antibacterial Potential of Methanol, Ethanol and Aqueous Extracts from Seed, Bark and Leaf of Bauhinia tomentosa L.

“…In contrast, the two other strains K. pneumonia (Gram -ve) and S. thermophilus (Gram + ve) were inhibited more by the methanol extracts followed by aqueous and ethanol extracts. A number of other studies on the effects of different plant extracts for their antimicrobial potential demonstrated the both contradictory as well as similar results (Mishra et al 2013;El-Moula et al 2019;Chalghoumi et al 2020). These differences in the antibacterial activities of the plant parts can be due to differences in the phytochemical composition of the part used and the solvents employed to extract these.…”

Antibacterial Effects of Fenugreek, Wheat and Hot Red Pepper Seeds and Their Germs Extract on Inhibiting Staphylococcus aureus and Enterobacter cloacae Growth

“…They are a well resource of essential amino acids mainly leucine, lysine and tryptophan. The general hypothesis that germination improves in vitro protein digestibility, as well as fat absorption capacity [31,32]. The germination process decreases the levels of total unsaturated fatty acids, total lipid, triglycerides, phospholipids and unsaponifiable matter, while those of saturated fatty acids are increased [33].…”