Effect of extracts from bean (phaseolus vulgaris) and field bean (vicia faba) varieties on intestinal D‐glucose transport in rat in vivo

Abstract

Saline extract solutions from seeds of both Phaseolus vulgaris and Vicia faba varieties were prepared and tested for their total polyphenolic content and effect on intestinal D-Glucose transport across rat ileum in viva. Intestinal D-ghCOSe transport was significantly reduced (P< 0.001) by the presence of seed-extracts in the intestinal loop, regardless of the source of the extract. The order of potency (Phaseolus vulgaris, var. artropurpurea > Vicia faba, var. caballur > Vicia ,faha, var. minor > Phaseolus vulgaris, var. albus) is closely related to the polyphenolic content. Clear differences in the mode of action of the extracts were also observed. Extracts with appreciable polyphenolic content caused both a reversible and a lasting inhibitory effect on glucose transport, whilst extracts with negligible polyphenolic content only induced a reversible inhibition. Polyamide treatment of the extracts decreased their ability to inhibit glucose transport, but their inhibitory effect was not completely abolished by the treatment, further suggesting the presence of some other anti-absorptive factor(s) in the seeds.

Comparative Effect of Green Tea, Chokeberry and Honeysuckle Polyphenols on Nutrients and Mineral Absorption and Digestibility in Rats

“…This was in agreement with results of other in sacco polyphenol experiments [14,[26][27][28] . Generally, in other studies, a dose-dependent decrease of nutrient absorption was recorded.…”

“…It was suggested that an inhibition of absorption was a result of protein denaturation on the surface of brush border cells [7] . However, later studies did not observe any alterations on the surface of the intestine of rats perfused with liquid containing bean polyphenols [14] . A potential mechanism of the inhibitory action of polyphenols takes place inside the mucosa and includes a significant dose-dependent decrease of brush border enzymatic activity [10] , the most important of which seems to be a reduction of Na + ,K + pump activity in the mucosa.…”

“…Therefore, an elevated intake of polyphenols may result in toxicological effects for the organism [11][12][13] . A confirmation of the possible toxic activity of polyphenols are results of in sacco experiments on isolated segments of the intestinal tract where polyphenols, after a short passage, strongly reduce nutrient absorption from the intestinal lumen [14,15] . However, little is known about the physiological and nutritional consequences of consumption of similar amounts of polyphenolic extracts in daily diets over a longer period of time.…”

Effect of feeding tannic acid and kidney bean (Phaseolus vulgaris) on the intestinal absorption of D‐galactose and L‐leucine in chickens

“…In-vitro intestinal transport of galactose was also reduced in birds fed on the diets containing tannic acid and in those fed the RKB diet. Similar results have been found in rats and chickens fed on other raw legume diets Santidrian 1981;Motilva et a/ 1983). The mechanism by which this inhibitory effect occurs remains to be completely understood although several hypotheses have been put forward (Mitjavila et al 1970(Mitjavila et al , 1977Ribereau-Gayon 1972).…”

Influence of Diets Containing Different Levels of <i>Vicia faba </i>L. as Source of Protein on Body Protein Composition and Nitrogen Balance of Growing Rats

“…Care was taken to prepare isoenergetic diets to avoid Using adequate techniques, we reported that the intestinal absorption was diminished in rats on V. faba, which is in reasonable agreement with the elevated values of fecal nitrogen and the decreased urinary nitrogen excretion in these animals [16][17][18].…”