Background and Objective: Fried fish may be contaminated with carcinogenic Polycyclic Aromatic Hydrocarbons (PAHs) during the frying process. This research aimed to determine the effect of frying at various temperatures and time intervals on the concentration of PAHs generated in fried catfish. Materials and Methods: Samples were gutted, washed and fried in hot vegetable oil at 160, 170, 180, 190, 200, 210, 220 and 230EC for 6,9,12,15 and 18 min at each temperature to determine the PAHs and free fatty acid (FFA). The PAHs content was extracted using an ultrasonicator and analyzed with gas chromatography coupled with a flame ionization detector. Results: The results showed that fish samples processed at 200EC for 15 min recorded the highest concentration of total PAHs 564.17 mg kgG 1 followed by samples processed at 200EC for 6 min with total PAHs content of 304.79 mg kgG 1 . The lowest total PAHs concentration was generated at 210EC for 9 and 12 min with concentrations of 18.16 and 23.57 mg kgG 1 , respectively. From the results of FFA obtained, FFA was highest at a temperature of 220EC, 15 min with the concentration of 25027.56 mg kgG 1 followed by the concentration generated at 210EC, 18 min. The lowest concentration of FFA was obtained at 190EC, 6 min with the concentration of 116.05 mg kgG 1 followed by the concentration generated at 200EC 15 min which is 135.28 mg kgG 1 . Conclusion: The proximate analysis results showed that there is an increase in the protein, fat and ash content as the temperature increases.