Effect of fatty acids from ethanol extract of Moringa oleifera seeds on kidney function impairment and oxidative stress induced by gentamicin in rats

Nafiu, Aliyat O.; Akomolafe, Rufus O.; Alabi, Quadri K.; Idowu, Clement O.; Odujoko, Oluwole

doi:10.1016/j.biopha.2019.109154

Cited by 27 publications

(21 citation statements)

References 40 publications

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“…In the present study, serum calcium was reduced signi cantly and a non-signi cant decrease in serum sodium and potassium in gentamicin treated rats were observed. These observations on serum sodium and potassium levels although non-signi cant, are in agreement with those of Na u et al (2019) showing that serum sodium and potassium reduction were observed in gentamicin treated rats, indicative of lesions in renal tubular epithelium and despair of Na + -K + ATPase by gentamicin. Studies in animal and human have demonstrated that gentamicin and other aminoglycosides impair calcium transport in the renal tubules and, in some instances, result in a signi cant increase in urinary calcium excretion and in that way causes hypocalcemia (Randjelović et al 2017).…”

Section: Discussionsupporting

confidence: 91%

“…Mechanisms of GMinduced nephrotoxicity comprises free radicals generation, increase lipids peroxidation, decrease activity of endogenous antioxidant, renal in ammation characterized by macrophage in ltration and subsequent release of proin ammatory cytokines associated with the activation of the stress-induced NF-B (Kalayarasan et al 2009;Balakumar et al 2010;Lee et al 2012). Several approaches have been tried to reduce gentamicin-nephrotoxicity by utilizing various natural antioxidants, such as Moringa oleifera seeds, Rosmarinus o cinalis and Thymus vulgaris (Abdel-Azeem et al 2017;Na u et al 2019), due to their effectiveness, minimal side effects in clinical use, and relatively low costs. Herbs have many phytochemicals, many of which possess antioxidant activity and can be promising agents against gentamicin-toxicity.…”

Section: Introductionmentioning

confidence: 99%

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Aqueous extract from Cinnamomum zeylanicum (Lauraceae) stem-bark ameliorates gentamicin-induced nephrotoxicity in rats by modulating oxidative stress and inflammatory markers

Atsamo

SONGMENE

Donfack

et al. 2021

Preprint

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Nephropathies and especially nephrotoxicity has become one of serious cause of life threatening condition, because of intensive exposure to xenobiotic either by environmental pollution or drug abuse. The present study was undertaken to assess the protective effects of Cinnamomum zeylanicum stem-bark aqueous extract (AECZ) on gentamicin-induced nephrotoxicity. AECZ was prepared by maceration in water and tested orally at the doses of 200 and 400 mg/kg/day to prevent gentamicin induced nephropathies in male Wistar rats. Gentamicin (100 mg/kg/day) was administered for 14 consecutive days by intraperitoneal route, concomitantly with AECZ or silymarin (50 mg/kg/day) used as reference drug. Animal body weight was monitored during the treatment. After the last treatment of the 14 th day, a 24h urine was collected and animals were sacrificed. Blood was collected for the evaluation of hematological and renal function biomarkers. The homogenate of one kidney was used to assess oxidative stress markers and pro-inflammatory cytokine, while the other one was fixed in formaldehyde for histopathological studies. Gentamicin decreased body weight, serum total proteins and calcemia, but increased kidneys relative weight, serum creatinine, urea and uric acid. Moreover, the levels of reduced glutathione, catalase and superoxide dismutase activities were decreased, while an increase in malondialdehyde, proinflammatory cytokines (TNFα, IL-1β, IL-6) and nitrites were observed in negative control group as compared to normal control. Histological analysis of the kidney revealed the presence of tubular necrosis, glomerular degeneration and macrophage infiltration in gentamicin treated group. All these impairment parameters were prevented by AECZ and silymarin treatments.AECZ has a protective effect against gentamicin-induced nephrotoxicity. The antioxidant and anti-inflammatory potentials of this extract may highly contribute to its nephroprotective activity.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Aqueous extract from Cinnamomum zeylanicum (Lauraceae) stem-bark ameliorates gentamicin-induced nephrotoxicity in rats by modulating oxidative stress and inflammatory markers

Atsamo

SONGMENE

Donfack

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The clinical application of gentamicin is seriously limited by its ototoxic nephrotoxicity, which leads to the decrease of its antibacterial activity 21 . Although the specific mechanism of GM-induced nephrotoxicity remains unclear, there is increasing evidence that oxidative stress, inflammatory response and cell apoptosis play an important role in GM-induced nephrotoxicity 22 . Antioxidant therapy can help prevent this nephrotoxic effect 23 .…”

Section: Discussionmentioning

confidence: 99%

Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway

Wang

Wang³

et al. 2020

Sci Rep

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Although gentamicin is widely used as an antibiotic in clinical practice, it also has some side-effects, such as acute kidney injury, which is a common condition caused by the abuse of gentamicin. Sika deer antler protein (SDAPR) can antagonize drug-induced AKI. Since SDAPR is recognized as an effective part of velvet antler, its components were further separated. Two components named SDAP1 and SDAP2 were obtained. The protective effects of SDAPR, SDAP1 and SDAP2 on GM-induced cytotoxicity to HEK293 and its potential mechanisms were studied. MTT and xCELLigence Real-Time cell analysis showed that SDAPR, SDAP1 and SDAP2 could protect HEK293 cells from GM toxicity. Similarly, SDAPR, SDAP1 and SDAP2 can reduce ROS level, reduce oxidative stress and improve inflammation Further studies have shown that SDAPR, SDAP1 and SDAP2 upregulate the Nrf2/HO-1 pathway by increasing the expression of Nrf2 and HO-1, and down-regulate the NF-κB pathway by reducing the protein expression of NF-κB. Annexin V/PI flow cytometry and Hoechst 33258 staining showed that SDAPR, SDAP1 and SDAP2 inhibited GM-induced apoptosis in HEK293 cells. Western blot analysis showed SDAPR, SDAP1 and SDAP2 decreased expression level of Bax and Cleaved-caspase-3, and increased the expression level of Bcl-2. In addition, we examined the feasibility of SDAP1 and SDAP1 to avoid kidney injury in a GM mouse model. In conclusion, SDAPR, SDAP1 and SDAP2 can be used to prevent GM-induced HEK293 cytotoxicity, probably because they have strong anti-oxidative stress, anti-inflammatory and anti-apoptotic effects. And SDAP1 and SDAP2 can inhibit GM-induced acute kidney injury in mice.

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“…Mechanisms of GM-induced nephrotoxicity comprise free radicals generation, increase lipids peroxidation, and decrease activity of endogenous antioxidant, renal inflammation characterized by macrophage infiltration, and subsequent release of proinflammatory cytokines associated with the activation of the stress-induced NF-κB, acute tubular necrosis, and glomerular congestion, resulting in diminished glomerular filtration rate and renal dysfunction [5][6][7]. Several approaches have been tried to reduce gentamicin nephrotoxicity by using various natural antioxidants, such as the use of Moringa oleifera seeds, Rosmarinus officinalis, and ymus vulgaris [8,9]. is is due to their effectiveness, minimal side effects in clinical use, and relatively low costs.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Extract from Cinnamomum zeylanicum (Lauraceae) Stem Bark Ameliorates Gentamicin-Induced Nephrotoxicity in Rats by Modulating Oxidative Stress and Inflammatory Markers

Atsamo

Songmene

Donfack

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Nephropathies and especially nephrotoxicity have become one of the serious causes of life-threatening conditions because of intensive exposure to xenobiotic whether by environmental pollution or by drug abuse. The present study was undertaken to assess the protective effects of Cinnamomum zeylanicum stem bark aqueous extract (AECZ) on gentamicin-induced nephrotoxicity. AECZ was prepared by maceration in water and tested orally at the doses of 200 and 400 mg/kg/day to prevent gentamicin-induced nephropathies in male Wistar rats. Gentamicin (100 mg/kg/day) was administered for 14 consecutive days by intraperitoneal route, concomitantly with AECZ or silymarin (50 mg/kg/day) used as reference drug. Animal body weight was monitored during the treatment. After the last treatment on the 14th day, animals were sacrificed. Blood was collected for the evaluation of hematological and renal function biomarkers. The homogenate of one kidney was used to assess oxidative stress markers and proinflammatory cytokines, while the other one was fixed in formaldehyde for histopathological studies. Gentamicin decreased body weight, serum total proteins, and calcium level but increased kidneys’ relative weight, serum creatinine, urea, and uric acid. Moreover, the levels of reduced glutathione, catalase, and superoxide dismutase activities were decreased, while an increase in malondialdehyde, proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and nitrites was observed in the negative control group as compared to normal control. Histological analysis of the kidney revealed the presence of tubular necrosis, glomerular degeneration, and macrophage infiltration in the gentamicin-treated group. All these impairment parameters were prevented by AECZ and silymarin treatments. AECZ has a protective effect against gentamicin-induced nephrotoxicity. The antioxidant and anti-inflammatory potentials of this extract may highly contribute to its nephroprotective activity.

show abstract

Effect of fatty acids from ethanol extract of Moringa oleifera seeds on kidney function impairment and oxidative stress induced by gentamicin in rats

Cited by 27 publications

References 40 publications

Aqueous extract from Cinnamomum zeylanicum (Lauraceae) stem-bark ameliorates gentamicin-induced nephrotoxicity in rats by modulating oxidative stress and inflammatory markers

Aqueous extract from Cinnamomum zeylanicum (Lauraceae) stem-bark ameliorates gentamicin-induced nephrotoxicity in rats by modulating oxidative stress and inflammatory markers

Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway

Aqueous Extract from Cinnamomum zeylanicum (Lauraceae) Stem Bark Ameliorates Gentamicin-Induced Nephrotoxicity in Rats by Modulating Oxidative Stress and Inflammatory Markers

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