Effect of fatty acids on the permeability barrier of model and biological membranes

Arouri, Ahmad; Lauritsen, Kira Emilie; Nielsen, Henriette Lai; Mouritsen, Ole G.

doi:10.1016/j.chemphyslip.2016.10.001

Cited by 28 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On this basis, it can be proposed that DPPC bilayers containing high percentages of LAH are tightly packed and would possibly release the fatty acid at a slow rate. Interestingly, most studies of the interaction between carboxylic acids and phosphatidylcholines containing 1,2-saturated acyl chains where strong hydrogen bonding was observed were performed with fatty acids and phospholipids bearing the same chain length [37]. In the present case, the fact that LAH is four carbons shorter than DPPC might lead one to expect more of a disordering effect (loss of cooperativity) in the acyl chain region.…”

Section: Resultsmentioning

confidence: 82%

Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Farkuh

Hennies

Nunes

et al. 2019

Heliyon

View full text Add to dashboard Cite

Lauric acid (LAH) strongly inhibits the growth of acne-causing bacteria. LAH is essentially water-insoluble and the solubility of laurate (LA) salts are medium and temperature dependent. Hence, LAH/LA preparations are difficult to formulate. Here we fully characterized phospholipid vesicles containing up to 50 mol% LAH. Vesicles of dipalmitoylphosphatidylcholine (DPPC) containing LAH, at pHs 7.4 and 5.0, were characterized measuring size, charge, bilayer phase transition temperature (Tm) and permeability of water-soluble probes. Small angle X-ray scattering and cryotransmission electron microscopy showed multilamellar vesicles at low LAH %. Increasing LAH % had a negligible effect on particle size. An internal aqueous compartment in all vesicle's preparations, even at equimolar DPPC: LAH fractions, was demonstrated using water-soluble probes. At pH 5.0, the interaction between DPPC and LAH increased the Tm and phase transition cooperativity showing a single lipid phase formed by hydrogen-bonded DPPC: LAH complexes. At pH 7.4, vesicles containing 50 mol% LAH exhibited distinct phases, ascribed to complex formation between LAH and LA or LAH and DPPC. LAH incorporated in the vesicles minimally permeated a skin preparation at both pHs, indicating that the primary sites of LAH solubilization were the skin layers. These results provide the foundations for developing processes and products containing DPPC: LAH.

show abstract

Section: Resultsmentioning

confidence: 82%

Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Farkuh

Hennies

Nunes

et al. 2019

Heliyon

View full text Add to dashboard Cite

show abstract

“…SCD5 silencing, as previously reported for SCD1 [ 33 ], most likely determines a decrease in membrane oleic acid concentration and a parallel accumulation of SFAs. The membrane perturbing effect of increasing SFA concentration has been found to significantly reduce cell viability, even in MCF-7 cells, most likely via the induction of necrosis and cell lysis [ 34 , 35 ]. In particular, the SFA palmitic acid has been found to act directly via endoplasmic reticulum (ER) to initiate a lipotoxic response [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5

et al. 2018

View full text Add to dashboard Cite

The influence of cell membrane fluidity on cancer progression has been established in different solid tumors. We previously reported that “cancer-associated fibroblasts” (CAFs) induced epithelial-mesenchymal transition and increased cell membrane fluidity and migration in poorly (MCF-7) and highly invasive (MDA-MB-231) breast cancer cells. We also found that the membrane fluidity regulating enzyme stearoyl-CoA desaturase 1 (SCD1) was upregulated in tumor cells co-cultured with CAFs and established its essential role for both intrinsic and CAF-driven tumor cell motility. Here, we further explored the mechanisms involved in the SCD1-based modulation of breast cancer cell migration and investigated the role of the other human SCD isoform, SCD5. We showed that the addition of oleic acid, the main SCD1 product, nullified the inhibitory effects produced on MCF-7 and MDA-MB-231 cell migration by SCD1 depletion (pharmacological or siRNA-based). Conversely, SCD5 seemed not involved in the regulation of cancer cell motility. Interestingly, a clear induction of necrosis was observed as a result of the depletion of SCD5 in MCF-7 cells, where the expression of SCD5 was found to be upregulated by CAFs. The necrotic effect was rescued by a 48-h treatment of cells with oleic acid. These results provide further insights in understanding the role of SCD1 in both intrinsic and CAF-stimulated mammary tumor cell migration, unveiling the metabolic basis of this desaturase-triggered effect. Moreover, our data suggest the ability of CAFs to promote the maintenance of tumor cell survival by the induction of SCD5 levels.

show abstract

“…The effect of different lipids on the mechanical properties and dynamic behaviour of bilayer membranes has been studied extensively in the past using experiments 7,8 , analytical theory 9,10 and molecular dynamics (MD) simulations [11][12][13][14][15][16][17][18][19][20][21][22] . In particular, Ding et al 21 have employed atomistic simulations to quantify the effects of changes in the ratio of lamellar vs. nonlamellar lipids on the physical properties of bilayer membranes.…”

mentioning

confidence: 99%

“…For the former, common parameters are the size [43][44][45][46] , shape 44,47 , solubility [47][48][49] and charge 50,51 . For the latter, published research examined the effect of the length of lipid chains 15,[52][53][54][55] , unsaturation [56][57][58][59] and different headgroups [60][61][62] . Also, many studies have focused on the addition of cholesterol 48,[62][63][64][65][66][67][68][69] .…”

mentioning

confidence: 99%

Effects of lipid composition on membrane permeation

et al. 2018

View full text Add to dashboard Cite

show abstract

Effect of fatty acids on the permeability barrier of model and biological membranes

Cited by 28 publications

References 46 publications

Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5

Effects of lipid composition on membrane permeation

Contact Info

Product

Resources

About