Effect of Fatty Acids on Interaction between Liposome and Amyloid β-peptide

Abstract

In 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)/fatty acid (FA) system, the analysis of the interaction between the DMPC/FA liposome and amyloid β-peptide (Aβ) was carried out by using the quartz crystal microbalance (QCM) method, together with the analysis of the compression isotherms of the DMPC/FA monolayer. The DMPC/stearic acid (SA) liposome showed the highest intensity in its interaction with Aβ through the QCM method although the slight interaction between DMPC/linoleic acids (LAs) liposome and Aβ was obtained. The compression isotherm analysis of the lipid monolayers revealed that the DMPC/SA monolayers underwent the distinct phase transfer, with which the interaction between both molecules changed from repulsive to attractive, while DMPC/linoleic acid monolayers did not.

Immobilization of intact liposomes on solid surfaces: A quartz crystal microbalance study

“…NHS residues were deactivated in 1 M 2-aminoethanol solution for 30 min. The stearic acid-containing DMPC liposomes were immobilized on the amino group layer by using DMTMM as catalysis as in a previous report [23].…”