Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers

Abstract

<jats:p>Aim: The study investigated the effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal (FCPMO) on the immune responses, antioxidative status, biochemical parameters, and intestinal ecology of broiler chickens. Materials and Methods: Four hundred Lohmann broiler chickens were distributed to four groups of diets including CONT (corn-soybean-based feed with no additive), BACI (corn-soybean-based diet supplemented with 0.1% zinc bacitracin), FERM (diet containing 20% FCPMO), and FERB (diet containing 20% FCPMO and added with 0.1% Bacillus subtilis). At days 4, 14, and 19, the chicks were vaccinated using commercial Newcastle disease-infectious bursal disease (ND-IBD), IBD, and ND vaccines, respectively. At day 35, blood was sampled and digesta was obtained from the ileum and caecum. Furthermore, the duodenal segment was obtained. Results: The BACI, FERM, and FERB groups had higher (p&lt;0.05) serum superoxide dismutase activity than control. The malondialdehyde was lower (p=0.07) in BACI, FERM, and FERB than that in CONT. The BACI and FERM had lower (p&lt;0.05) leukocytes and lymphocytes than CONT. The hemoglobin, erythrocytes, and hematocrit were lower (p&lt;0.05) in BACI and FERM than those in CONT and FERB. Serum total triglyceride was lower (p&lt;0.05) in FERM and FERB than that in CONT. The FERM and FERB had higher (p&lt;0.05) albumin levels. Serum globulin level was lower (p&lt;0.05) in FERB than that in BACI, but did not differ from that in CONT and FERM. The numbers of coliform, lactose-negative enterobacteria and enterobacteria were lower (p&lt;0.05) in FERB than that in other treatment groups. Crypt depth (CD) was higher (p&lt;0.05) in FERM, while the villi height to CD ratio was lower (p&lt;0.05) in FERM than that in CONT and FERB. The treatments showed no effect (p&gt;0.05) on cecal volatile fatty acids production. Conclusion: Feeding FCPMO improved immune responses, antioxidative status, and physiological conditions, but had less effect on the intestinal ecology of broilers.</jats:p>

Performances of Crossbred Chickens Fed Fermented Papaya Leaf and Seed Powder at High Stocking Density

“…In this study, FPLS seemed to contribute to the increase SOD level of the ICC. This assumption was supported by our previous study showing the increased SOD con- centration in broilers fed with fermented cassava pulp and Moringa oleifera leaf powder (Sugiharto et al, 2020b). Our result showed that HDL level was lower in HSD+mE than those in LSD, HSD, and HSD+FPLS.…”

Blood and Intestine Profile of Broilers Fed Averrhoa bilimbi Fruit, Wheat Bran, and Yeast Blends

“…Taking all the above facts together, the decreased levels of erythrocytes and haemoglobin in additive fed-broilers seemed to be the response of the birds toward the decreased potential of infections. This finding was actually in agreement with Sugiharto et al (2020), reporting the decrease in erythrocytes and haemoglobin levels, concomitant with the decrease in leukocytes and lymphocytes, in broiler chickens when provided with antibiotics, zinc bacitracin, and fermented feed. March 2022 51…”

Effect of Addition Sinbiotic Isolate Pediococcus pentosaceus Strain N6 and Cassava Peel on Immunity Organs and Total Microbes in the Small Intestine of Super Native Chicken

“…This value indicates that the total lactic acid bacteria is close to the normal range [12]. [10] stated that the total lactic acid bacteria that grow in the digestive tract of chickens is 4.5 x 10 8 CFU/ml. The addition of 450 ml of sinbiotic resulted in significantly different total microbial colonies with 150 ml and 300 ml administration.…”

“…Table 4, it can be seen that the highest mean total microbial colony (TPC) was in the P3 treatment (basal ration + sinbiotic 450 ml/kg ration) which was 3.42 x 10 8 CFU/ml and the lowest average total microbial colony (TPC) was found in P0 (basal ration without sinbiotic) is 2.00 x 10 the total lactic acid bacteria is close to the normal range. [10] stated that the total lactic acid bacteria that grow in the digestive tract of chickens is 4.5 x 10 8 CFU/ml. The addition of 450 ml of sinbiotic resulted in significantly different total microbial colonies with 150 ml and 300 ml administration.…”