Effect of Feeding Fructooligosaccharides and Enterococcus faecium and Their Interaction on Digestibility, Blood, and Immune Parameters of Adult Horses

Saeidi, E; Yarahmadi, Hossein Mansoori; Fakhraei, Jafar; Mojahedi, Somayeh

doi:10.1016/j.jevs.2021.103410

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Azzaz et al (2022) stated that milk production, feed efficiency, and in vitro nutrient degradation were all increased when cows were given daily supplements of E. faecium (both isolated and commercial) at a rate of 2 g/kg DM feed. The different result reported by Saeidi et al (2021) that feeding adult horses fructooligosaccharides and E. faecium reduced triglyceride and cholesterol contents and decreased fecal pH, but had no effect on nutrient digestibilities.…”

Section: Rumen Dry Matter Organic Matter and Crude Fiber Digestibilitymentioning

confidence: 75%

Improving Rumen Fermentation by Inoculation of Cellulolytic Bacteria Isolated from Anoa, Bison, Muntjak and Timor Deer Faecal

Suharti,

Cahyani,

Pramarta

et al. 2023

American Journal of Animal and Veterinary Sciences

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Tropical endemic herbivores such as anoa (Bubalus depressicornis), bison (Bos javanicus), muntjak (Muntiacus muntjak), and Timor deer (Rusa timorensis), can digest various types of fibrous feed because their digestive tract has high cellulolytic bacteria and those can be found in their feces. Previously, we isolated and identified cellulolytic bacteria from the feces of anoa, bison, muntjak, and Timor deer. This study aimed to examine the use of a consortium of cellulolytic bacteria originating from feces of anoa, bison, muntjak, and Timor deer in the in vitro rumen fermentation and their effect on digestibility and fermentation properties. The in vitro study was conducted using beef cattle rumen fluid with 4 treatments and 5 replications in a Completely Randomized Design (CRD). The treatment including Control (T0) = Forage: Concentrate 60: 40, T1 = T0+10 5 CFU/mL cellulolytic bacteria, T2 = T0 + 10 6 CFU/mL cellulolytic bacteria, T1 = T0+10 7 CFU/mL cellulolytic bacteria. Variables measured were dry and organic matter digestibility, crude fiber digestibility, rumen pH, formation of Ammonia (NH3), Volatile Fatty Acids (VFA), microbial population, and methane estimation. The results showed that the addition of cellulolytic bacterial consortium isolated from anoa, bison, muntjak, and Timor deer feces increased dry matter digestibility, crude fiber digestibility, total VFA production, and total bacterial population. Rumen pH, ammonia production, proportional VFA, and methane were similar among treatments. Inoculation of the cellulolytic bacteria consortium at level 10 6 CFU/mL was the optimum level to improve fiber digestibility and had a beneficial effect for ruminants that fed high-fiber feed.

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Section: Rumen Dry Matter Organic Matter and Crude Fiber Digestibilitymentioning

confidence: 75%

Improving Rumen Fermentation by Inoculation of Cellulolytic Bacteria Isolated from Anoa, Bison, Muntjak and Timor Deer Faecal

Suharti,

Cahyani,

Pramarta

et al. 2023

American Journal of Animal and Veterinary Sciences

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“…Almost all individual biochemical results were within physiological levels, except higher cholesterol concentration in dog 6 (7.7 vs. 5.8 mmol/L, day 0 vs. 7), the activity of alanine aminotransferase in dog 4 (0.584 vs. 0.221 µkat/L) and the phosphorus concentration in dogs 1 and 4 (3.6, 4.8 vs. 1.9, 1.5 mmol/L). A hypocholesterolemic effect is commonly observed after application of many enterococcal probiotic strains (in vitro, in humans, in animals) [31][32][33], which is attributed to enzymatic deconjugation of bile acids by bile-salt hydrolase in probiotics, to the ability to bind cholesterol onto cellular surface, to incorporation into cellular membranes or to the conversion of cholesterol to coprostanol [34]. Optimisation of biochemical parameters, such as cholesterol and ALT, have also been detected after application of the probiotic bacteria Lactobacillus fermentum CCM7421, which was applied to different groups of dogs (healthy or suffering from gastrointestinal disorders, [35]).…”

Section: Discussionmentioning

confidence: 99%

Application of Canine-Derived Enterococcus faecium DSM 32820 in Dogs with Acute Idiopathic Diarrhoea

et al. 2022

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Modulation of the intestinal microbiota through the application of probiotic bacteria is currently one possible way to improve gastrointestinal health in dogs. Knowledge on the efficacy of lactic acid bacteria in a diarrhoeic disorder of dogs is still spreading; however, the used or commercialized strains are often not of canine origin. In this study, E. faecium DSM 32820 strain (a canine isolate selected in our laboratory based on safety and probiotic criteria) was fed to nine dogs suffering from acute non-haemorrhagic diarrhoea at a dose of 1×109 CFU/ml for 7 days. Samples of feces and blood were taken on day 0 and 7. Evaluation of the CIBDAI (Canine Intestinal Bowel Disease activity index) score showed significantly lower vomiting frequency, stool frequency, stool consistency and weight loss at day 7 compared to day 0 (P<0.05). The 16S rRNA gene analysis revealed Firmicutes as the predominant phylum on both sampling days (72.0% vs. 67.9%, day 0 and 7) followed by Proteobacteria (13.4% vs. 6.0%), Actinobacteria (10.0% vs 13.5%), Fusobacteria (4.2% vs. 2.3%) and Bacteroidetes (0.4% vs. 10.4%). The abundance of family Erysipelotrichiaceae was higher on day 7 compared to the initial levels (P<0.05). Among 19 detected fecal enzymatic activities, five (α-chymotrypsin, β-glucuronidase, α-fucosidase, β-galactosidase, N-acetyl-glucosaminidase) were changed (P<0.05). After the application of the DSM 32820 strain, mean fecal dry matter was significantly higher on day 7 compared to baseline (P< 0.05). Although hematological and biochemical parameters in the blood were not significantly different on average, individual values of certain parameters in several dogs were improved.

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“…Several studies evaluating the impact of prebiotics on the horse-hindgut microbiome have been reported, but most of them either enrolled a low number of horses per group, included many variables, evaluated different products, or were performed in vitro [ 152 , 153 , 154 , 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 ]. Furthermore, it is not rare for the private companies producing those compounds to sponsor the studies, and therefore results should be interpreted with particular caution as they tend to emphasize the beneficial changes in the microbiome.…”

Section: Techniques Of Microbiota Manipulationmentioning

confidence: 99%

Current Understanding of Equine Gut Dysbiosis and Microbiota Manipulation Techniques: Comparison with Current Knowledge in Other Species

Boucher,

Leduc,

Leclère

et al. 2024

Animals

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Understanding the importance of intestinal microbiota in horses and the factors influencing its composition have been the focus of many studies over the past few years. Factors such as age, diet, antibiotic administration, and geographic location can affect the gut microbiota. The intra- and inter-individual variability of fecal microbiota in horses complicates its interpretation and has hindered the establishment of a clear definition for dysbiosis. Although a definitive causal relationship between gut dysbiosis in horses and diseases has not been clearly identified, recent research suggests that dysbiosis may play a role in the pathogenesis of various conditions, such as colitis and asthma. Prebiotics, probiotics, and fecal microbiota transplantation to modulate the horse’s gastrointestinal tract may eventually be considered a valuable tool for preventing or treating diseases, such as antibiotic-induced colitis. This article aims to summarize the current knowledge on the importance of intestinal microbiota in horses and factors influencing its composition, and also to review the published literature on methods for detecting dysbiosis while discussing the efficacy of gut microbiota manipulation in horses.

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Effect of Feeding Fructooligosaccharides and Enterococcus faecium and Their Interaction on Digestibility, Blood, and Immune Parameters of Adult Horses

Cited by 5 publications

References 39 publications

Improving Rumen Fermentation by Inoculation of Cellulolytic Bacteria Isolated from Anoa, Bison, Muntjak and Timor Deer Faecal

Improving Rumen Fermentation by Inoculation of Cellulolytic Bacteria Isolated from Anoa, Bison, Muntjak and Timor Deer Faecal

Application of Canine-Derived Enterococcus faecium DSM 32820 in Dogs with Acute Idiopathic Diarrhoea

Current Understanding of Equine Gut Dysbiosis and Microbiota Manipulation Techniques: Comparison with Current Knowledge in Other Species

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