Effect of feeding high-temperature, microtime-treated diets with different lipid sources on conjugated linoleic acid formation in finishing Hanwoo steers1

Abstract

The present study was conducted to examine the effects of different plant oils or plant oil mixtures and high-temperature, microtime processing (HTMT) on the CLA content in Hanwoo steers. Experiment 1, consisting of 3 in vitro trials, was conducted to determine how the biohydrogenation of C18 fatty acids and CLA production were affected by fat sources (tallow, soybean oil, linseed oil, or mixtures of soybean oil and linseed oil) or HTMT treatment in the rumen fluid. The results showed that HTMT was capable of protecting unsaturated fatty acids from biohydrogenation by ruminal bacteria. The HTMT-treated diet containing 4% linseed oil (LU) and a supplement containing 2% linseed oil and 1% soybean oil treated with HTMT + 1% soybean oil (L(2)S(1)U+S(1)) produced an increased quantity of trans-11 C18:1 and cis-9, trans-11 CLA, and a reduced quantity of trans-10, cis-12 CLA. Based on these results, in vivo studies (Exp. 2) were conducted with LU and L(2)S(1)U+S(1). These 2 treatments increased the content of cis-9, trans-11 CLA in LM compared with the control diet. The content of trans-10, cis-12 CLA in subcutaneous fat was also increased in the L(2)S(1)U+S(1) treatment compared with other treatments. The subcutaneous fat thickness in the LU treatment was decreased compared with the L(2)S(1)U+S(1) treatment. The LU treatment significantly decreased fatty acid synthase expression but simultaneously increased leptin expression. In this report, we showed that diets containing LU and L(2)S(1)U+S(1) were capable of increasing CLA in the intramuscular fat of beef.

Meat Quality Traits of Longissimus Muscle of Hanwoo Steers as a Function of Interaction between Slaughter Endpoint and Chiller Ageing

“…Additionally, SFA, MUFA and PUFA were 49.08, 42.84 and 6.86 g/100 g, respectively. It was noted that the Hanwoo in the study of Xu et al (2008) had more SFA and C18:2n-6 than the Hanwoo in our experiment. This may be ascribed to the difference in the diet used and the higher proportion of concentrate and roughage given to the animal in the experiment of Xu et al (2008).…”

“…The total fatty acid was comprised of 43% SFA, 53% MUFA and 3% PUFA. Xu et al (2008) observed that in 26 mon old Hanwoo steers (644 kg) fed ground concentrate and alfalfa hay on a 9:1 ratio for a 2 mon feeding period, the quantity (g/100 g) of the major fatty acid components of m. longissimus are as follows: C16 (26.49), C16:1 (2.78), C18 (16.77), C18:1n-9 (34.57), C18:2n-6 (6.52), C18:3n-3 (0.20). Additionally, SFA, MUFA and PUFA were 49.08, 42.84 and 6.86 g/100 g, respectively.…”

Lipid oxidation products of heated soybeans as a possible cause of protection from ruminal biohydrogenation

“…As already shown in studies with 130 to 160°C roasted oilseeds 8, 9 or with heated oil 1, 30, heating offered a partial protection of PUFA from BH in this study. With extruded oilseeds, some protection of PUFA was sometimes observed 6, 7 but not always 11, 12.…”

Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species

“…The high ATGL activity in BAT together with the impaired thermogenesis in ATGL-null mice, while HSL-null mice exhibited normal thermogenesis, suggest that ATGL is essential for the provision of FA as substrate for BAT thermogenesis (Zechner et al, 2009). To date, only HSL has been identified in bovine WAT through its mRNA abundance (Bonnet et al, 1998;Sumner and Mcnamara, 2007;Xu et al, 2008) and its activity (Sprinkle et al, 1998;Kazala et al, 2003). The thermogenic protein UCP1, together with enzymes involved in the citric acid cycle and the respiratory chain, are expressed in bovine foetal BAT, allowing FA oxidation and heat production (Casteilla et al, 1989;Smith et al, 2004;Hocquette et al, 2006).…”