Effect of feeding rice whole crop silage on growth rate, levels of vitamin A, β‐carotene, vitamin E and IGF‐1 in plasma and skeletal muscle protein degradation in Japanese black calves

Abstract

This study evaluated the effects of rice whole crop silage (RWCS) on growth, plasma levels of vitamin A, β-carotene, vitamin E and IGF-1, and expression of genes involved in muscle protein degradation and synthesis in Japanese Black calves. Eleven calves were divided into RWCS (fed RWCS ad libitum and concentrate, n = 5) and control groups (fed hay ad libitum and concentrate, n = 6). Final body weight and dairy gain were significantly larger in the RWCS group compared with the control group. Plasma β-carotene and vitamin E concentrations were significantly higher in the RWCS group compared with control group. Although plasma vitamin E concentration in the RWCS group significantly increased from 4 to 9 months of age, it did not increase in the control group. At 6 months of age in the RWCS group, ubiquitin B (p < 0.05) and calpain 1 (p = 0.097) mRNA expression were lower than control group, but they were not different between groups at 9 months of age. These results indicate that RWCS increases plasma β-carotene level and promotes muscle growth because of a decrease in the rate of protein degradation, but the effect is lost with the increase in plasma vitamin E level. K E Y W O R D Sβ-carotene, growth rate, Japanese Black calves, myofiber properties, rice whole crop silage