Effect of Feeding Selenium-Fertilized Alfalfa Hay on Performance of Weaned Beef Calves

Hall, Jean A.; Bobe, Gerd; Hunter, Janice K.; Vorachek, William R.; Stewart, W. C.; Vanegas, Jorge A.; Estill, Charles T.; Mosher, Wayne D.; Pirelli, Gene J.

doi:10.1371/journal.pone.0058188

Cited by 51 publications

(56 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Calves were bled after feeding Se-enriched alfalfa hay for 6 or 7 weeks, as part of a study that has been previously reported [26]. The experimental protocol was reviewed and approved by the Oregon State University Animal Care and Use Committee.…”

Section: Whole-blood Collectionmentioning

confidence: 99%

Reference gene selection for quantitative PCR studies in bovine neutrophils

Vorachek¹,

Bobe²,

Hall³

2013

ABB

Self Cite

View full text Add to dashboard Cite

Reference genes are essential for studying mRNA expression with quantitative PCR (qPCR). We investigated 11 candidate whole-blood neutrophil reference genes (ACTB, B2M, G6PD, GAPDH, GYPC, HPRT, PGK1, RPL19, SDHA, TFRC, and YWHAZ) for beef calves, both males and females, with or without selenium supplementation. Initial screening was based on gene expression level (<28 Cq cycles), variability (SD < 1.5 Cq cycles), excluded GYPC and TFRC from further analysis. Expression stability of the remaining genes was evaluated using four software programs: geNorm, NormFinder, BestKeeper, and the comparative delta Cq method. The neutrophil reference genes, YWHAZ, PGK1, and RPL19, consistently ranked among the top four most stable genes under these experimental conditions. The commonly used reference genes, ACTB and HPRT, were not reliable, underscoring the need to validate neutrophil reference genes under different experimental conditions. Multiple reference genes rather than a single gene may provide more robust and reliable results. The best pair of reference genes in whole-blood neutrophils from beef calves overall was PGK1|YWHAZ.

show abstract

Section: Whole-blood Collectionmentioning

confidence: 99%

Reference gene selection for quantitative PCR studies in bovine neutrophils

Vorachek¹,

Bobe²,

Hall³

2013

ABB

Self Cite

View full text Add to dashboard Cite

show abstract

“…Oxidative stress is a primary mechanism by which ROS influences biological processes and may contribute to the pathogenesis of disease by modifying the expression of proinflammatory genes (Hall et al . ,). We compared the effect of supranutritional dietary Se on antioxidant capacity in immune organs of the chicken and the results showed increased ( P < 0.05) activities of GSH‐Px, T‐AOC and SOD mainly occurred in early stages (at days 14 and 28) (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…Feeding Se‐enriched forage improves antibody titers in mature beef cows (Hall et al . ,), and elevates whole‐blood Se concentrations in weaned beef calves (Hall et al ).…”

Section: Introductionmentioning

confidence: 99%

Supranutritional dietary selenium depressed expression of selenoprotein genes in three immune organs of broilers

Tang

Huang

Wang

et al. 2016

Animal Science Journal

View full text Add to dashboard Cite

The objective of this study was to investigate the effects of supranutritional dietary selenium (Se) on selenoproteins expression in three immune organs of chickens. A total of 160 1-day-old male Cobb broilers were randomly divided into two groups and fed a Se-deficient corn-soybean basal diet supplemented with 0.3 (adequate) and 3.0 (excess) mg/kg Se for 42 days. Immune organs were collected, and effects of supranutritional Se on messenger RNA abundance of 23 selenoprotein genes and eight inflammation-related genes were compared at day 42. Also enzyme activities were measured at days 14, 28 and 42. The results showed supranutritional dietary Se depressed growth performance of chicken and down-regulated nine and three selenoprotein genes in thymus and spleen, respectively, and only Sepp1 was up-regulated in the bursa of Fabricius. Also three, three and seven inflammation-related genes were up-regulated in three organs, respectively. Supranutritional Se elevated (P < 0.05) activities of superoxidase dismutase, total antioxidant capacity and glutathione peroxidase, mainly in early stages. In summary, supranutritional Se resulted in down-regulation of selenoprotein genes and up-regulation of inflammation-related genes in three immune organs of chicken, which indicated potential roles of those selenoprotein genes in immune organs of the chicken.

show abstract

“…We previously showed in beef cows and recently weaned beef calves that feeding Se-enriched hay improves vaccination responses [24,25,58]. Studies are warranted to determine whether other forms of organic Se, such as Se-fortified forage, are as effective as Se yeast in reducing parasite infection.…”

Section: Discussionmentioning

confidence: 99%

Effect of Selenium Yeast Supplementation on Naturally Acquired Parasitic Infection in Ewes

Hooper

Bobe

Vorachek

et al. 2014

Biol Trace Elem Res

Self Cite

View full text Add to dashboard Cite

Gastrointestinal parasites cause substantial economic losses in pasture-based sheep production systems. Supranutritional organic selenium (Se) supplementation may be beneficial because it improves immune responses to pathogens. To evaluate the effect of Se-yeast supplementation on gastrointestinal parasite load, 30 ewes per treatment group were drenched weekly with no Se, 4.9 mg Se/week as Se yeast (maximum FDA-allowed concentration), or supranutritional concentrations of Se yeast (14.7 and 24.5 mg Se/week) starting early fall for 85 weeks. Fecal samples were collected at weeks 63, 66, 78, and 84 and counted for total trichostrongyle-type eggs and Haemonchus contortus eggs (in samples with ≥200 trichostrongyle eggs/g feces). During breeding season (fall), ewes were kept on pasture; ewes receiving 24.5 mg Se/week had lower fecal trichostrongyle egg counts (93 ± 40 eggs/g feces) compared with ewes receiving no Se (537 ± 257 eggs/g feces; P = 0.007) or ewes receiving 4.9 mg Se/week as Se yeast (398 ± 208 eggs/g feces; P = 0.03). In winter, fecal trichostrongyle egg counts decreased, and group differences were not apparent. During lambing season (spring), ewes were kept in the barn and fecal trichostrongyle egg counts increased, although no group differences were observed. However, none of the ewes receiving supranutritional Se yeast, and with trichostrongyle egg counts ≥200 eggs/g of feces, but four of the ewes receiving lower Se dosages had H. contortus egg counts ≥1,000 eggs/g feces (P = 0.04). Our results suggest that supranutritional Se-yeast supplementation may enhance resistance to naturally occurring H. contortus gastrointestinal parasitism in sheep.

show abstract

Effect of Feeding Selenium-Fertilized Alfalfa Hay on Performance of Weaned Beef Calves

Cited by 51 publications

References 24 publications

Reference gene selection for quantitative PCR studies in bovine neutrophils

Reference gene selection for quantitative PCR studies in bovine neutrophils

Supranutritional dietary selenium depressed expression of selenoprotein genes in three immune organs of broilers

Effect of Selenium Yeast Supplementation on Naturally Acquired Parasitic Infection in Ewes

Contact Info

Product

Resources

About