Effect of femtosecond laser radiation on morphofunctional state of neoplasm in vitro

Abstract

The effect of femtosecond laser radiation provided by the Erbium fiber laser with the pulse duration of 10 -13 s, peak and average power of 6,0±0,3 kW and 1,26±0,15 mW, respectively, wavelength λ of 1,55 μm has been studied in the experiments on rat ascitic ovarian tumor cell in vitro. Irradiation has been performed at the average intensity of 0,033±0.002 mW/cm 2 at two expositions under femtosecond laser radiation of 600 and 900 s. The membrane topology and rigidity of the cancer cells have been estimated with the Scanning probe microscopy (SolverPro, NT-MDT, Russia). Besides, the viability and apoptosis of the cancer cells have been estimated. Free-radical processes and antioxidant enzyme activity have been studied in cancer cell lysate. Femtosecond laser irradiation was established to increases the activity of the "Lipoperoxidationantioxidants" system in neoplasm, enhance the apoptosis, decrease the viability, and change cancer cell membrane topology and rigidity in vitro depending on the energy density of the irradiation.