Effect of fenbenzyl and fenbendazole on peroxide intensity oxidation of lipids in the blood of dogs in experimental toxocariasis

Abstract

Toxocariasis is a chronic helminthic disease caused by the larval stages of the nematode Toxocara canis. The problem of toxocariasis is important for both humane and veterinary medicine. Therefore, the aim of the study was to investigate the effect of fenbenzyl and fenbendazole on the intensity of lipid peroxidation in the blood of dogs during experimental infestation with the pathogen toxocariasis. The experiments were performed on 18 dogs, two to four months of age, and three groups of six animals were formed in each: control and two experimental groups. Puppies of all groups were experimentally infected with the pathogen toxocariasis at a dose of 5.000 invasive T. canis eggs per kg of body weight. The control group of dogs was as untreated control. Puppies of the first experimental group were fed the drug “Fenbendazole” at a dose of 150 mg per 3 kg of animal weight once a day for three days in a single dose. Puppies of the second experimental group were fed the drug “Fenbenzyl” at a dose of 350 mg per 3 kg of animal weight once a day for three days. In the clinical manifestation of the disease, toxocariasis secretes metabolic products that promote the formation of large amounts of free radicals, which in turn enhance the initiation of lipid peroxidation processes. With the use of fenbendazole and fenbenzyl, a probable decrease in these products was found starting from the 15th day of the experiment. It should be noted that the lowest level of diene conjugates and TBA-active products was in the blood of dogs of the second experimental group at 25 and 30 days of the experiment. Inhibition of lipid peroxidation processes in the treatment of dogs with fenbenzyl is due to the activation in animals of metabolic processes involving enzymes, including antioxidant enzymes that catalyze the processes of peroxidation and phosphorylation, as well as enhancing erythropoietic function. On the 30th day of the experiment, the level of diene conjugates in the blood of the second experimental group was 0.29 ± 0.02 IU/ml, and TBA-active products – 25.2 ± 0.40 μmol/l.

Effect of Fenbenzyl and Fenbendazole on the Antioxidant Status in Dogs During Experimental Infection With Causative Agent of Toxocariasis

“…It is known that the intestinal form of the disease is caused by mature Toxocara, and the larvae -visceral. In the process of migration, larvae, as well as their metabolites, can cause severe multiorgan damage up to death (Moisieieva et al, 2017;Said et al, 2018;Stybel et al, 2021). However, some mechanisms of activation of lipid-free radical oxidation processes in the development of toxocariasis in dogs and their relationship with the body's defense systems, especially the immune system, which is closely related to the antioxidant defense system in animals, remain unclear.…”

Contemporary approaches to assessment of the body's condition of dogs with gastrointestinal pathologies based on the intensity of the inflammato-ry process and the level of endogenous intoxication

“…Any animal disease is created by typical pathological processes (TPP), such as inflammation, endogenous intoxication, impaired immune protection, metabolism disorder, power supply, etc. (Kryzhanovskij, 2002;Borgarelli et al, 2004;Borgarelli et al, 2012;Varkholiak & Gutyj, 2020;Stybel et al, 2021;Konstantinidis et al, 2021). Effective and timely detection of the presence and determination of the direction of the TPP is the key to success in the prevention, treatment, and rehabilitation of multiple diseases.…”