Effect of Field Inoculation with
 Sinorhizobium meliloti
 L33 on the Composition of Bacterial Communities in Rhizospheres of a Target Plant (
 Medicago sativa
 ) and a Non-Target Plant (
 Chenopodium album
 )—Linking of 16S rRNA Gene-Based Single-Strand Conformation Polymorphism Community Profiles to the Diversity of Cultivated Bacteria

Schwieger, Frank; Tebbe, Christoph C.

doi:10.1128/aem.66.8.3556-3565.2000

Cited by 129 publications

(70 citation statements)

References 67 publications

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“…significantly affected bacterial communities in the rhizosphere of alfalfa (Babic et al 2008;Schwieger and Tebbe 2000). Some rhizosphere microbiome inoculations could modify bacterial communities and change plant flowering time (Panke-Buisse et al 2015).…”

Section: The Effects Of G Liquefaciens Qzr14 Inoculation On Soil Bacmentioning

confidence: 99%

The persistence and performance of phosphate-solubilizing Gluconacetobacter liquefaciens qzr14 in a cucumber soil

Wang

Yin

et al. 2017

3 Biotech

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The persistence and performance of plant growth-promoting microorganisms (PGPMs) in soil are considered critical features for effectiveness, yet they are poorly understood. Here, we investigated the colonization and activity of a new PGPM, phosphate-solubilizing Gluconacetobacter liquefaciens qzr14, in a pot culture experiment using cucumber as test crop for 20 days. The number of G. liquefaciens and bacterial diversity in the rhizosphere and bulk soil were monitored by real-time PCR and DGGE, respectively. Soil phosphorus and cucumber biomass were also examined. G. liquefaciens qzr14 effectively colonized the rhizosphere soil (bacterial density ranging from 2.70 9 10 8 to 1.18 9 10 9 copies per gram dry soil). G. liquefaciens qzr14 inoculation had significantly positive effects on bacterial diversity (BD) of the rhizosphere and bulk soil and the ratio of soluble phosphorus to total phosphorus (SP/TP). The number of G. liquefaciens in the rhizosphere soil was significantly related to SP/TP and the BD of the rhizosphere and bulk soil. BD in rhizosphere soil was significantly related to SP/TP and BD in bulk soil. Based on the results of correlation analysis, we inferred that the introduced G. liquefaciens qzr14 effectively colonized the rhizosphere of cucumber, and then expanded its bacterial community by solubilizing soil phosphorus. The expanded bacterial communities might promote cucumber growth by some new functions.

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Section: The Effects Of G Liquefaciens Qzr14 Inoculation On Soil Bacmentioning

confidence: 99%

The persistence and performance of phosphate-solubilizing Gluconacetobacter liquefaciens qzr14 in a cucumber soil

Wang

Yin

et al. 2017

3 Biotech

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“…The low persistence of a released Rhizobium is not necessarily linked to its being genetically impaired. For example, a recA-minus derivative of S. meliloti showed a stability similar to that of its wild-type, as it declined from 10 6 to 10 4 cfu.g −1 of soil in two years time (Schwieger and Tebbe, 2000). Climatic conditions such as heavy rain after release negatively affect persistence (Hirsch, 1996), and so do cold winters, as shown in the boreal zone for GM R. galegae (Pitkajarvi et al, 2003).…”

Section: Discussionmentioning

confidence: 92%

Long term evaluation of field-released genetically modified rhizobia

Corich

Vendramin

Vian

et al. 2007

Environ. Biosafety Res.

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This is the report of the first open field release of genetically modified microorganisms (GMMs) in Italy. It covers ten years of monitoring, and follows in-field GMM dynamics from strain release to disappearance below detection limits, as well as assessment of impact on resident microorganisms. The bacteria released belong to the nitrogen fixing legume endosymbiont Rhizobium leguminosarum bv. viciae, and were engineered with non-agronomically-proficient traits, in order to assess their behavior and fate without GMM-specific positive feedback from the plant. A DNA cassette containing mercury resistance and ß-galactosidase genes was introduced in either plasmid-borne or chromosomally integrated versions, in order to test the resulting strain stability. A synthetic promoter was used to drive the lacZ gene, conferring high catabolic activity to the GMM. Two different wild-type Rhizobium backgrounds were tested, comparing a non-indigenous vs. an indigenous, highly competitive strain. The latter had much greater persistence, since it was able to survive and establish at technically detectable levels for over four years after release. Selection factors, such as reiterated presence of the plant host, or lactose substrate supply, enhanced long-term survival to different extents. The lactose treatment showed that even a single trophic supplementation can surpass the benefits of symbiotic interaction for a period of several years. Concerning impact, the GMMs did not alter substantially the other soil community general microbiota. However, there were some significant differences in microbiota as a consequence of the Rhizobium inoculation. This effect was observed with either the WT or GMM, and was more evident in the release of the indigenous Rhizobium. Moreover, as the indigenous GMM had its parental, dominant wild-type in the same soil, it was possible to evaluate to what extent the GMM version could result in parent displacement ("self-impact"), and how much the two rhizobia would additively contribute to nodulation.

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“…To assess the relevance of an intact recA gene function for the strains' survival in soil, laboratory and greenhouse-based microcosm experiments (Hagen et al, 1997;Niemann et al, 1997a;Schwieger et al, 1997), experiments in field lysimeters (Schwieger et al 2000b) and finally, a field release experiment was performed (Selbitschka et al 2006). In particular, the field study which was conducted in field plots of the Federal Research Institute for Agriculture (FAL; now Johann Heinrich von Thünen-Institute, Federal Research Institute for Rural Areas, Forestry and Fisheries) in Northern Germany in the year 1995 had raised several questions which were addressed in the present field study.…”

Section: Fate and Ecological Interactions Of Firefly Luc Gene-tagged mentioning

confidence: 99%

“…The bacteria were isolated by squashing the surface sterilized nodules in parallel onto TY agar medium and TY containing streptomycin (100 mg l ). S. meliloti cells isolated from nodules were resuspended in 50 µl water supplied with 10 µl luciferin solution and tested for bioluminescence in a luminometer (Schwieger et al 2000b). Molecular characterization of alfalfa nodulating rhizobia from soil samples PCR detection and discrimination of the S. meliloti isolates was performed as described by Dammann-Kalinowski et al (1996) using two PCR primers, one specific for the luc gene, one specific for the DNA region downstream of the recA coding region.…”

Section: Isolation Of Alfalfa Nodulating Rhizobia From Soil Samplesmentioning

confidence: 99%

Fate and Ecological Interactions of Firefly Luc Gene-Tagged Sinorhizobium Meliloti 2011-Bacteria in Soil Inhabited by High Levels of Indigenous Alfalfa Nodulating Populations

Selbitschka

Keller²,

Dresing

et al. 2008

Ecological genetics

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A field study was conducted with genetically modified sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA+), both tagged with the firefly luciferase luc gene as an identification marker. The strains' fate was studied over a time period of five years. Both strains were rapidly outcompeted for alfalfa nodulation by an indigenous population. In summary, this study demonstrates the usefulness of tagging bacteria designed for environmental releases by the firefly luciferase gene and the high resilience of soil bacteria to allow the establishment of foreign bacterial populations.Key words: alfalfa, Sinorhizobium meliloti, luc gene-tagging, nodulation competitiveness, indigenous populations, field releases of genetically modified bacteria. FATE AND ECOLOGICAL INTERACTIONS OF FIREFLY LUC GENE-TAGGED sINORhIzObIUm melIlOTI 2011-BACTERIA IN SOIL INHABITED BY HIGH LEVELS OF INDIGENOUS ALFALFA NODULATING POPULATIONSA field study was conducted with genetically modified Sinorhizobium meliloti strains L1 (RecA -) and L33 (RecA + ), both tagged with the firefly luciferase luc gene as an identification marker. The strains were released as peat-based inocula at densities of approx. 10 6 cfu g -1 soil in replicate and randomised field plots at a field site in Bavaria (Germany). The strains' fate was studied over a time period of five years. During the first six weeks after inoculation, the viable count of both luc tagged strains sharply dropped for more than two orders of magnitude. Thereafter, the titer of both strains more or less steadily declined within the next five years to approx. 10 2 cfu g -1. No significant differences in the survival of strains L1 (RecA -) and L33 (RecA + ) were noted at most sampling dates demonstrating that the recA gene function was dispensable under the prevalent environmental conditions. Both strains were rapidly outcompeted for alfalfa nodulation by an indigenous population which was well-established in the field site soil. RecA -strain L1 was slightly faster outcompeted than its isogenic RecA + counterpart L33. In order to mimic normal agricultural practice, one year after the field release the non-host plant rye was grown in a total of 21 of the 49 field plots. Cultivation of rye had neither a negative nor a positive effect on the survival of strains L1 and L33 in these plots, respectively. Both strains showed the same survival capabilities, comparable to that of the field plots were the host plant alfalfa was grown. The diversity of the indigenous population was characterized by employing the Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR fingerprint method. Typing of more than 340 indigenous isolates recovered from alfalfa root nodules throughout the monitoring period of five years revealed the presence of five dominant strain types accounting for approximately 67 % of the isolates of the indigenous alfalfa nodulating population. Nearly one third of the dominant isolates displayed the Rhizobium sp. Or191 fingerprint pattern. IS fingerprinting of representatives of the various s...

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Cited by 129 publications

References 67 publications

The persistence and performance of phosphate-solubilizing Gluconacetobacter liquefaciens qzr14 in a cucumber soil

The persistence and performance of phosphate-solubilizing Gluconacetobacter liquefaciens qzr14 in a cucumber soil

Long term evaluation of field-released genetically modified rhizobia

Fate and Ecological Interactions of Firefly Luc Gene-Tagged Sinorhizobium Meliloti 2011-Bacteria in Soil Inhabited by High Levels of Indigenous Alfalfa Nodulating Populations

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