Effect of fisetin and probiotic supplementation on erythrocyte osmotic fragility, malondialdehyde concentration and superoxide dismutase activity in broiler chickens exposed to heat stress

Abstract

The aim of the study was to evaluate effects of fisetin and probiotic on erythrocyte osmotic fragility (EOF), malondialdehyde (MDA) and superoxide dismutase (SOD) in broiler chickens exposed to heat stress. Sixty day-old broilers were divided into: Group I (control) given distilled water; Group II, fisetin (5 mg/kg); Group III, probiotic Saccharomyces cerevisiae (4.125 × 106 cfu/100 ml); and Group IV, fisetin (5 mg/kg) + probiotic (4.125 × 106 cfu/100 ml) orally for 7 days. Blood samples collected from 42-day-old birds were evaluated for EOF, serum MDA concentration and SOD activity. Percentage EOF at 0.5% NaCl was lower (P<0.05) in fisetin, probiotic and fisetin + probiotic groups (34.26 ± 0.98%, 35.65 ± 0.81% and 34.25 ± 1.98%, respectively) than in controls (48.42 ± 0.40%). The MDA concentrations in broiler chickens administered with fisetin (14.37 ± 1.15 nmol/l), probiotic (5.66 ± 1.06 nmol/l) and fisetin + probiotic (4.136 ± 0.58 nmol/l) were lower (P<0.05) than in controls (22.64 ± 2.95 nmol/l). Activities of SOD were higher (P<0.05) in fisetin, probiotic and fisetin + probiotic broiler chickens (6.34 ± 0.24 IU/l, 5.67 ± 0.09 IU/l and 5.93 ± 0.13 IU/l, respectively) than in controls (5.37 ± 0.09 IU/l). Fisetin + probiotic ameliorated oxidative stress changes in broiler chickens better than fisetin or probiotic alone. In conclusion, administration of fisetin or probiotic and, especially their combination, decreased EOF, lipoperoxidation and increased superoxide dismutase activity in broiler chickens exposed to heat stress.

Dietary probiotics as a strategy for improving growth performance, intestinal efficacy, immunity, and antioxidant capacity of white Pekin ducks fed with different levels of CP

“…In the present study, probiotic implementation mediates a significant increase in SOD and CAT activities with optimal and suboptimal dietary CP, suggesting an enhanced antioxidant potential. Several reports have detected boosted antioxidant enzyme (SOD and CAT) levels in poultry provided with different probiotics ( Aluwong et al., 2013 ; Abudabos et al., 2016 ; Bai et al., 2017 ; Ogbuagu et al., 2018 ; Deraz et al., 2019 ).…”

Therapeutic Potential of Natural Products in the Treatment of Renal Cell Carcinoma: A Review

“…Though not reported in RCC, flavonols (kaempferol, fisetin and myricetin) play a role in reducing risks for advanced prostate cancer but show no effect on bladder cancer susceptibility [ 89 ]. Interestingly, fisetin, as well as chelerythrine are also reported to be important supplements for poultry to avoid heat stress-associated morbidities and spotty liver disease, respectively [ 90 , 91 ]. Lastly, animal studies on Lycium barbarum polysaccharides, osthole and thymoquinone have also shown satisfactory tolerability in vivo and those products hold promise to the manufacture of relevant supplements [ 92 , 93 , 94 ].…”

Cloacal temperature responses of broiler chickens administered with fisetin and probiotic (Saccharomyces cerevisiae) and exposed to heat stress

“…This might subsequently lead to low immunity, decrease performance, suppression, high morbidity, and death; hence, antioxidants, such as fisetin and probiotic could be helpful during the season when administered adequately. Oxidative stress is a resultant of heat stress which consequently, enhances reactive oxygen species (ROS) production (Ogbuagu et al, 2018;Shakeri et al, 2020;Sumanu et al, 2019), therefore, the findings of this study serves as the basis for modulating the detrimental effects of heat stress in animals via fisetin and/or probiotic administration.…”