Effect of Fish Oil Treatment on Gentamicin Nephrotoxicity in Rats

Ali, Basharat; Bashir, A.A.

doi:10.1159/000177831

Cited by 23 publications

(8 citation statements)

References 9 publications

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“…Recently, dietary FO has been shown to protect against acetaminophen (paracetamol)induced hepatotoxicity (Speck and Lauterburgh 1991), ethanol-induced gastric mucosal injury in rats (Leung 1992), and a number of inflammatory diseases such as lupus nephritis (Chandrasekar and Fernandes 1994). Preliminary reports also showed partial protection by dietary FO x-3 fatty acids against cyclosporine/GMinduced nephrotoxicity (Thakkar et al 2000;Ali and Bashir 1994); however, the mechanism involved was not studied in detail. Our results thus support the rationale that x-3 fatty acids enriched FO may be an effective dietary supplementation in the management of cisplatin nephrotoxicity and other pathologies in which antioxidant defense mechanisms are impaired.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of cisplatin-induced nephrotoxicity by grape seed extract and fish oil is mediated by lowering oxidative stress and DNA damage

et al. 2013

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Cisplatin (CP) is a chemotherapeutic drug used in treatment of malignancies. However, its clinical utility is limited by nephrotoxicity. The purpose of the present study was to investigate the protective role of grape seed proanthocyanidin extract (GSPE) (100 mg/kg/day) or fish oil (FO) (5 ml/kg/day) against cisplatin induced nephrotoxicity in terms of biochemical parameters, oxidative stress and DNA damage. CP nephrotoxiciy is manifested by increased levels of serum creatinine, urea and uric acid, accompanied by their decrease in urine. Na, K and Ca levels were altered in both serum and urine. In addition, cisplatin caused a decrease in renal GSH, SH-group, SOD, GST, and Na-K-ATPase levels. However the levels of MDA, H2O2 and NO were increased. Also, we assessed the renal genotoxic potential of cisplatin as manifested by an increase in the tail length of DNA, tail intensity (DNA %) and tail moment. On the other hand, administration of GSPE or FO pre-cisplatin treatment ameliorated the current changes in most of the above tested parameters, particularly oxidative stress, endogenous antioxidant defense system and DNA damage indicating their curative effect. Thus, it can be concluded that the consumption of GSPE or FO might be useful for preventing nephrotoxicity caused by cisplatin treatment.

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Section: Discussionmentioning

confidence: 99%

Amelioration of cisplatin-induced nephrotoxicity by grape seed extract and fish oil is mediated by lowering oxidative stress and DNA damage

et al. 2013

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“…resulting in swelling, vacuolization and necrosis of epithelial cells, and accumulation of myelin-like bodies. 39 Furthermore, results of many studies have shown that altered concentrations of various biochemical indicators of oxidative stress in kidney tissue are due to GEN. 40 Because of the obvious responsibility of ROS in GENinduced renal damage, several antioxidant agents have been used to prevent GEN nephrotoxicity. 41 MK, a selective reversible cys-leukotriene-1 receptor (LTD4 receptor) antagonist, is used in the treatment of asthma and is reported to reduce airway eosinophilic inflammation in this disease.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Montelukast Which Is Cysteinyl-Leukotriene Receptor Antagonist on Gentamicin-Induced Nephrotoxicity and Oxidative Damage in Rat Kidney

et al. 2013

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Nephrotoxicity is a major complication of gentamicin (GEN). We aimed to evaluate the potential protective effect of montelukast (MK) against GEN-induced nephrotoxicity in rats. Thirty-two rats were randomly divided into four groups, each consisting of eight animals as follows: (1) the rats were control; (2) intraperitoneally injected with GEN 14 consecutive days (100 mg/kg/day); (3) treated with GEN plus distilled water via nasogastric gavage for 14 days; and (4) treated with GEN plus MK (10 mg/kg/day) for 14 days. After 15 days, rats were killed and their kidneys were taken and blood analysis was performed. Twenty-four hours urine collections were obtained in standard metabolic cages a day before the rats were killed. Tubular necrosis and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in the other part of kidneys. Statistical analyses were made by the chi-square test and analysis of variance. Serum urea and creatinine levels were significantly higher in rats treated with GEN alone, than the rats in control and GEN þ MK groups. The GSH levels in renal tissue of only GEN-treated rats were significantly lower than those in control group, and administration of MK to GEN-treated rats significantly increased the level of GSH. The group that was given GEN and MK had significantly lower MDA and NO levels in kidney cortex tissue than those that was given GEN alone. In rats treated with GEN þ MK, despite the presence of mild tubular degeneration and tubular necrosis are less severe, and glomeruli maintained a better morphology when compared with GEN group. We can say that MK prevents kidney damage with antioxidant effect, independently of NO.

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“…GEN is a widely used aminoglycoside antibiotic and has been shown to cause marked histological damage in particular to renal proximal convoluted tubules, 30,31 resulting in swelling, vacuolization, and necrosis of epithelial cells and accumulation of myelin-like bodies. 32 Furthermore, the results of many studies have shown that altered concentrations of various biochemical indicators of oxidative stress in kidney tissue are due to GEN. 33 Because of the obvious responsibility of ROS in GEN-induced renal damage, several antioxidant agents have been used to prevent GEN nephrotoxicity. 34 PE is rich in antioxidants of the polyphenolic class, which includes tannins and anthocyanins.…”

Section: Discussionmentioning

confidence: 99%

Pomegranate Extract Attenuates Gentamicin-Induced Nephrotoxicity in Rats by Reducing Oxidative Stress

et al. 2012

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Nephrotoxicity is a major complication of gentamicin (GEN), which is widely used in the treatment of severe Gramnegative infections. Reactive oxygen species are important mediators of GEN-induced nephrotoxicity. Because of the strong antioxidant properties of pomegranate extract (PE), we evaluated the protective effect of PE against GEN-induced nephrotoxicity. Thirty-two adult male rats were randomly divided into four equal groups: (1) controls; (2) treated with GEN for 14 consecutive days (100 mg/kg/day); (3) treated with GEN plus distilled water; and (4) treated with GEN plus PE (100 μL). After 15 days, the rats were killed and their kidneys were taken, and blood analysis was performed. Tubular necrosis and interstitial fibrosis scores were determined histopathologically; and biochemically, nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels in kidneys were determined. Urea, creatinine, Na þ , and K þ levels were investigated in the blood analysis. Statistical analyses were made by the chi-square test and analysis of variance. Serum urea and creatinine levels were significantly higher in rats treated with GEN alone than rats in the control and the GEN þ PE-treated groups. The GSH level in renal tissue of only GEN-treated rats was significantly lower than those in the control group, and administration of PE to GEN-treated rats significantly increased the level of GSH. The group that was given GEN and PE had significantly lower MDA levels in kidney cortex tissue than those given GEN alone. There was no significant difference of NO levels between the groups. In rats treated with GEN þ PE, despite the presence of mild tubular degeneration and tubular necrosis is less severe, and glomeruli maintained a better morphology when compared with the GEN-treated group. We think that PE prevents kidney damage by decreasing oxidative stress in kidney.

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Effect of Fish Oil Treatment on Gentamicin Nephrotoxicity in Rats

Cited by 23 publications

References 9 publications

Amelioration of cisplatin-induced nephrotoxicity by grape seed extract and fish oil is mediated by lowering oxidative stress and DNA damage

Amelioration of cisplatin-induced nephrotoxicity by grape seed extract and fish oil is mediated by lowering oxidative stress and DNA damage

Protective Effect of Montelukast Which Is Cysteinyl-Leukotriene Receptor Antagonist on Gentamicin-Induced Nephrotoxicity and Oxidative Damage in Rat Kidney

Pomegranate Extract Attenuates Gentamicin-Induced Nephrotoxicity in Rats by Reducing Oxidative Stress

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