Effect of Flavonoid-Rich Extract From Dalbergiella welwitschii Leaf on Redox, Cholinergic, Monoaminergic, and Purinergic Dysfunction in Oxidative Testicular Injury: Ex Vivo and In Silico Studies

Abstract

The antioxidant, cholinergic, monoaminergic, and purinergic activities of flavonoid-rich extract from Dalbergiella welwitschii leaf (FEDW) were investigated on oxidative testicular injury (ex vivo) due to the local report on the use of this plant as anti-testicular injury. Flavonoid extract was obtained from FEDW using a standard procedure. Five male albino rats were used, testes harvested and incubated with FeSO4 for accessing the cholinergic, monoaminergic, and purinergic activities of the FEDW (ex vivo). Testicular tissues incubated with FeSO4 demonstrated a significant decrease in antioxidant biomarkers, arginase, ATPase, ENTPDase, 5ʹ-nucleotidase, and PDE-5 activities, as well as Zn and sialic acid levels with an upsurge in malondialdehyde (MDA), and NO levels, myeloperoxidase, cholinesterases, monoamine oxidase (MAO), and angiotensin-converting enzyme (ACE) activities. Treatment of testicular tissues incubated with FeSO4 via different concentrations of FEDW significantly increased the activities of antioxidant, arginase, ATPase, E-NTPDase, 5ʹ-nucleotidase, phosphodiesterase-5 (PDE-5), as well as Zn and sialic acid levels with a significant decrease in MDA, nitric oxide (NO), myeloperoxidase, cholinesterases, MAO, and ACE levels. Molecular docking revealed the molecular interactions of cyclooxygenase-2 (COX-2) with ellagic acid, piperine, and caffeine with piperine and caffeine obeyed the druggability and pharmacokinetic. These findings point to FEDW as a possible potential for the treatment of oxidative testicular injury.

Antioxidant, antidiabetic, and anti-inflammatory activities of flavonoid-rich fractions of Solanum anguivi Lam. fruit: In vitro and ex vivo studies

“…The scavenging abilities of the flavonoid-rich fractions of S. anguivi could serve in managing diabetes mellitus, as free radicals are involved in the pathogenesis of diabetes mellitus. This results correlates with previously reported studies on antioxidant activities of flavonoid-rich extract of Syzygium cumini and Dalbergiella welwitschii [ 44 , 45 ].…”

“…The most prominent hepatoprotective effect was evident in fraction A. This finding correlates with earlier studies on the flavonoid-rich fraction of Dalbergiella welwitschii leaf [ 45 ].…”

Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model

“…However, no reports have comprehensively studied the impacts of Al NPs on testicular tissues of migratory locusts. On the other hand, previous investigations sought to discover antioxidant agents derived from plants, particularly those containing polyphenolic compounds, to curtail the toxicity of the nanoparticles and treat the oxidative testicular injury triggered by their indirect influence on cells existing in testes [ 18 , 19 ]. However, the antioxidant compounds extracted from the insects have received scant attention in empirical research.…”

“…To hinder the toxicological effects, in particular the oxidative stress engendered by Al 2 O 3 NPs, many endeavors have been dedicated to discovering natural products with intrinsic antioxidant capacities. The major studies focused on antioxidant-derived plants that contain bioactive polyphenolic compounds such as quercetin to reduce oxidative damage in hepatic cells after treatment with Al 2 O 3 NPs [ 17 , 18 , 19 ]. So far, very little attention has been paid to antioxidant-derived insects to tackle the deleterious consequences of the metal NPs.…”