Effect of flow regime on the architecture of a <i>Pseudomonas fluorescens</i> biofilm

Pereira, Maria Olívia; Kuehn, Martin; Wuertz, Stefan; Neu, Thomas R.; Melo, L. F.

doi:10.1002/bit.10189

Cited by 202 publications

(141 citation statements)

References 23 publications

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“…Ohl et al (2004) investigated the effect of varying fluid velocity on the density of biofilms grown for up to 80d. There was a strong relationship between the imposed velocity and measured density, an expected result based on prior reports (Vieira, 1993, Pereira et al, 2002a. However, the response time of the biofilm to increased or decreased velocities revealed insights into the consolidation process.…”

Section: Introductionsupporting

confidence: 72%

Tracer measurements reveal experimental evidence of biofilm consolidation

Casey

2007

Biotech & Bioengineering

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SummaryThe ability to simultaneously measure both biofilm thickness and the mass transfer coefficient of an inert tracer through it provides a powerful method to study biofilm development. In this communication previously published data has been collated to interpret global trends in biofilm structure during the transition towards steady-state. It appears that sudden changes in biofilm structure (directly related to the rate of change of biofilm mass transfer resistance) may occur following transitions in rate of biomass production. These observations are consistent with the concept of consolidation, recently introduced into spatially structured biofilm mathematical models to account for structural realignment of the biofilm under dynamic conditions.

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Section: Introductionsupporting

confidence: 72%

Tracer measurements reveal experimental evidence of biofilm consolidation

Casey

2007

Biotech & Bioengineering

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“…This system also allowed to uncouple the system residence time and the fluid velocity. The basic concept and characteristics of the Flow Cell were reported by Pereira et al (2002).…”

Section: Biofilm Monitoring Reactorsmentioning

confidence: 99%

Dynamics of drinking water biofilm in flow/non-flow conditions

2007

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A B S T R A C TDrinking water biofilm formation on polyvinyl chloride (PVC), cross-linked polyethylene (PEX), high density polyethylene (HDPE) and polypropylene (PP) was followed in three different reactors operating under stagnant or continuous flow regimes. After one week, a quasi-steady state was achieved where biofilm total cell numbers per unit surface area were not affected by fluctuations in the concentration of suspended cells. Metabolically active cells in biofilms were around 17-35% of the total cells and 6-18% were able to form colony units in R 2 A medium. Microbiological analysis showed that the adhesion material and reactor design did not affect significantly the biofilm growth. However, operating under continuous flow (0.8-1.9 Pa) or stagnant water had a significant effect on biofilm formation: in stagnant waters, biofilm grew to a less extent. By applying mass balances and an asymptotic biofilm formation model to data from biofilms grown on PVC and HDPE surfaces under turbulent flow, specific growth rates of bacteria in the biofilm were found to be similar for both materials (around 0.15 day À1) and much lower than the specific growth rates of suspended bacteria (around 1.8 day À1 ).

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“…From the hydrodynamic perspective, mean velocity values from the water column do not represent turbulence patterns and flow that vary significantly at biofilm scale (Moulin and Eiff 2012;Hodl et al 2014). On the other hand, research focusing on biofilm architecture and composition in relation to flow usually involves single-species biofilm mostly grown in miniature flow cells under non-natural flow conditions (Pereira et al 2002;Wagner et al 2010). This example illustrates that biostabilisation research still requires fundamental input from various perspectives to investigate the most relevant conditions and processes to deliver variables that would allow implementation in models at a later stage.…”

Section: The Ecosystem Approachmentioning

confidence: 99%

Form, function and physics: the ecology of biogenic stabilisation

et al. 2018

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Purpose The objective of this work is to better understand the role that biological mediation plays in the behaviour of fine sediments. This research is supported by developments in ecological theory recognising organisms as Becosystem engineers^and associated discussion of Bniche construction^, suggesting an evolutionary role for habitat modification by biological action. In addition, there is acknowledgement from engineering disciplines that something is missing from fine sediment transport predictions. Materials and methods Advances in technology continue to improve our ability to examine the small-scale 2D processes with large-scale effects in natural environments. Advanced molecular tools can be combined with state-of-the-art field and laboratory techniques to allow the discrimination of microbial biodiversity and the examination of their metabolic contribution to ecosystem function. This in turn can be related to highly resolved measurements and visualisation of flow dynamics. Results and discussion Recent laboratory and field work have led to a paradigm shift whereby hydraulic research has to embrace biology and biogeochemistry to unravel the highly complex issues around on fine sediment dynamics. Examples are provided illustrating traditional and more recent approaches including using multiple stressors in fully factorial designs in both the laboratory and the field to highlight the complexity of the interaction between biology and sediment dynamics in time and space. The next phase is likely to rely on advances in molecular analysis, metagenomics and metabolomics, to assess the functional role of microbial assemblages in sediment behaviour, including the nature and rate of polymer production by bacteria, the mechanism of their influence on sediment behaviour. Conclusions To fully understand how aquatic habitats will adjust to environmental change and to support the provision of various ecosystem services, we require a holistic approach. We must consider all aspects that control the distribution of sediment and the erosion-transport-deposition-consolidation cycle including biological and chemical processes, not just the physical. In particular, the role of microbial assemblages is now recognised as a significant factor deserving greater attention across disciplines.

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Effect of flow regime on the architecture of a Pseudomonas fluorescens biofilm

Cited by 202 publications

References 23 publications

Tracer measurements reveal experimental evidence of biofilm consolidation

Tracer measurements reveal experimental evidence of biofilm consolidation

Dynamics of drinking water biofilm in flow/non-flow conditions

Form, function and physics: the ecology of biogenic stabilisation

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