Effect of fluorescein dye concentration in oral cancer tissue: Statistical and spectroscopic analysis

Thapa, Pramila; Bhatt, Sunil; Mishra, Deepika; Mehta, Dalip Singh

doi:10.1016/j.pdpdt.2023.103889

Cited by 1 publication

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its distinctive greenish-yellow fluorescence under ultraviolet light renders it easily detectable in medical settings. Due to its intrinsic fluorescent properties and selective binding characteristics, has revolutionized diagnostic and therapeutic approaches in various medical disciplines [ 23 , 24 ]. Indeed, it takes an indispensable role in facilitating accurate disease diagnosis, guiding surgical interventions, and monitoring treatment efficacy in diverse medical fields, ranging from ophthalmology [ 25 , 26 ] to cardiology [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Kinetic Model of Fluorescein Release through Bioprinted Polylactic Acid Membrane

de Nigris,

Minó,

Cinelli

et al. 2024

Biomimetics

View full text Add to dashboard Cite

Polylactic acid (PLA)-based cylindrical membranes for the controlled release of fluorescein sodium salt (FS) were prepared by bioprinting on systems with an initial FS concentration of 0.003763 gdm−3 and 37.63 gdm−3, and the drug release process was monitored in a bath at 37 °C. Photographs, acquired at regular intervals during the process, revealed marked osmotic swelling of the polymer. Osmotic swelling consists in the enlargement of the polymer structure and due to the influx of water molecules across the membrane. The cylindrical PLA membrane starts to significantly swell once a certain threshold range is crossed. Important amounts of FS can dissolve under these radically changed circumstances, and the dissolved FS molecules are mobile enough to diffuse out of the cylinder, thus allowing drug release. As a matter of fact, in this investigation, we ascertained that polymer swelling promotes the mass transport phenomenon by altering the conditions for drug dissolution and diffusion, hence facilitating FS release after a specific lag time. Furthermore, in order to compare the release kinetics, the half-release time, t0.5, was taken into consideration. The data of this study evidence that, while increasing the initial concentration of FS by three orders of magnitude, the time parameter, t0.5, is only reduced by 5/6. In addition, the yield of the release process is drastically reduced due to the strong aggregation ability of the dye. Finally, it is demonstrated that a compressed exponential kinetic model fits the experimental data well despite the varying physical conditions.

show abstract