Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria–material interface: An in vitro model study

Mayanagi, Gen; Igarashi, Koei; Washio, Jumpei; Domon-Tawaraya, Hitomi; Takahashi, Nobuhiro

doi:10.1016/j.jdent.2013.11.006

Cited by 36 publications

(29 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experiences with the cariogenity of the selected kind of S. mutans have been reported previously [9,13,33,46]. As a consequence, in the chosen set up, distinct demineralization was evident already after short incubation times.…”

Section: Discussionmentioning

confidence: 74%

“…A monobacterial system was chosen as artificial mouth system to minimize falsifying influences [9]. Experiences with the cariogenity of the selected kind of S. mutans have been reported previously [9,13,33,46].…”

Section: Discussionmentioning

confidence: 99%

“…Resin composites are considered as successful dental biomaterials; however, irrespective of modern developments, they still shrink upon polymerization with durable adhesion to tooth hard tissues remaining the fundamental prerequisite for clinical success [4][5][6][7][8][9][10][11]. In the case of failing adhesion, gap formation imperils clinical outcome by staining, secondary caries, and finally pulpal damage [4,5,[12][13][14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of microparticulate silver addition in dental adhesives on secondary caries in vitro

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of microparticulate silver addition in dental adhesives on secondary caries in vitro

et al. 2015

View full text Add to dashboard Cite

show abstract

“…[9] In a study conducted by Chau et al, results suggest that the anti-cariogenic biofilm activity of GICs is closely correlated with their fluoride release rate during biofilm formation. [10,11] The antimicrobial activity of the GICs could be related to the synergistic action of acid and fluoride release of the cement …”

Section: Discussionmentioning

confidence: 99%

Effect of Acid and Fluoride Release from Four Glass Ionomer Cements on Streptococcus mutans: An In Vitro Study

Mahuli¹,

Mahuli²,

Ramanarayanan³

et al. 2015

IJDHC

View full text Add to dashboard Cite

Objectives:The objective was to evaluate fluoride and acid release from four glass ionomer cements (GICs) over time and to evaluate and compare their effect on Streptococcus mutans over time. Materials and Methods: The fluoride and acid release from the prepared samples (GICs, Miracle Mix, Fuji IX, d-tech ® , Ketac Molar) were measured daily using digital fluoride meter and pH meter, respectively, after placing them in deionized distilled water for 15 days. The water was replaced daily. The samples were aged by placing them in distilled water for 1, 3, 5, and 7 days. The antimicrobial activity was assessed by measuring the zone of inhibition around the samples (fresh and aged) placed on the bacterial cultures after 48 h of incubation. Mean and standard deviation were calculated. Pearson's correlation coefficient was used. P < 0.05 was considered statistically significant. Results: The antimicrobial activity of the four GICs was greatest immediately after the cements were mixed. The greatest fluoride and acid release was from d-tech. Conclusion: Both acid and fluoride release that contributed to the antibacterial activity of the Glass ionomer cements reduced significantly as the cement aged.

show abstract

“…Recently, we developed an experimental apparatus with a miniature ion-sensitive field-effect transistor (ISFET) pH electrode and used it to monitor the pH and to quantify the amount of fluoride at the interface between bacteria and fluoride-releasing materials, such as glass-ionomer cements and resin composites [Mayanagi et al, 2011[Mayanagi et al, , 2014. In the present study, we used this model to evaluate the pH at the interface between the tooth surface and Streptococcus mutans cells by simulating an oral environment in which acid-producing bacteria existed on the tooth surface.…”

mentioning

confidence: 99%

pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface

Mayanagi

Igarashi²,

Washio³

et al. 2017

Caries Res

Self Cite

View full text Add to dashboard Cite

Evaluating the physiochemical processes at the tooth surface/bacteria interface is important for elucidating the etiology of dental caries. This study aimed to compare the mineral solubility and protein degradation of coronal enamel (CE) and root dentin (RD), and investigate the involvement of dissolved components in bacteria-induced pH changes using a model of tooth/bacteria interface. An experimental apparatus forming a well was made of polymethyl methacrylate, and a bovine tooth (CE or RD) specimen was fixed at the bottom of the well. A miniature pH electrode was placed on the tooth, and Streptococcus mutans NCTC 10449 cells, grown in 0.5% glucose-containing complex medium, were packed into the well. The pH at the tooth/S. mutans interface was monitored continuously for 120 min after the addition of 0.5% glucose at 37°C. S. mutans cells were recovered from the wells, and the amounts of lactate and calcium were measured using a portable lactate meter and a fluorescent dye, respectively. Proteolytic activity was also evaluated fluorometrically. The pH of the RD/S. mutans interface was significantly higher than that of the CE/S. mutans interface (30 min: 6.37 ± 0.12 vs. 6.18 ± 0.11, 60 min: 6.08 ± 0.14 vs. 5.66 ± 0.27, 90 min: 5.49 ± 0.24 vs. 5.14 ± 0.22, p < 0.05). Greater amounts of calcium were dissolved from RD (3.19 ± 0.74 µg/mL) than from CE (1.84 ± 0.68 µg/mL; p < 0.05), while similar amounts of lactate were produced. Proteolytic activity was not detected at any of the interfaces. These results indicate that RD is more soluble to bacteria-induced acidification than CE. This method can contribute to the evaluation and development of caries-preventive materials.

show abstract

Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria–material interface: An in vitro model study

Cited by 36 publications

References 28 publications

Effect of microparticulate silver addition in dental adhesives on secondary caries in vitro

Effect of microparticulate silver addition in dental adhesives on secondary caries in vitro

Effect of Acid and Fluoride Release from Four Glass Ionomer Cements on Streptococcus mutans: An In Vitro Study

pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface

Contact Info

Product

Resources

About