Effect of Fluoride Varnish on Streptococcus mutans Count in Saliva of Caries Free Children Using Dentocult SM Strip Mutans Test: A Randomized Controlled Triple Blind Study

Abstract

Aims: The aim of this study was to estimate the count of Streptococcus mutans in saliva of caries free children using Dentocult SM strip mutans and to evaluate the effect of fluoride varnish on the Streptococcus mutans count in saliva of these caries free children.Methods and material: Thirty caries free children were selected for the study based on the information obtained from a questionnaire prepared. They were randomly assigned into the control group and the study group consisting of ten and twenty children respectively. Samples of saliva were collected using the saliva strips from the Dentocult SM kit and after incubation the presence of the Streptococcus mutans was evaluated using the manufacturers’ chart. The study group was subjected to Fluor Protector fluoride varnish application after 24 hours following which the samples were collected again.Results: The average Streptococcus mutans count in primary dentition of caries free children was in the range of 104 to 105 colony forming units/ml. The average Streptococcus mutans count in primary dentition of caries free children after Fluor Protector fluoride varnish application was below 104 colony forming units/ml.Conclusion: Fluor Protector fluoride varnish application showed a statistically significant reduction in the Streptococcus mutans count in saliva of the caries free children in the study group.

A comprehensive review of the application of probiotics and postbiotics in oral health

“…S. mutans has been introduced as the main species of caries-causing bacteria due to its strong acid production and high tolerance to acidic conditions ( Sales-Campos et al., 2019 ; Hasslöf and Stecksén-Blicks, 2020 ). Research has shown that the number of S. mutans in the saliva of people without caries is normally between 10 4 and 10 5 CFU/mL ( Deepti et al., 2008 ), But at 10 6 CFU/mL, the risk of caries increases significantly ( Klock and Krasse, 1979 ). In this regard, 10 5 CFU/mL has been considered the threshold of caries in clinical studies ( Caglar et al., 2008b ; Ghasemi et al., 2017 ; Bafna et al., 2018 ).…”

Assessment of Antibacterial Effectiveness of SDF and Fluoride Varnish Agents for Application in Pediatric Dentistry

“…S. mutans count in saliva was statistically significantly lower after using fluor protector fluoride varnish, according to Deepti et al . [ 16 ]…”

Effectiveness of Silver Diamine Fluoride in Arresting Early Childhood Caries in Myanmar Preschool Children

“…Co. Ltd., Japan) at baseline and at 6-month follow-up. Dentocult ® SM determined the colony forming units (CFU) of mutans streptococci in dental plaque and saliva [14,15]. Cariostat ® detected the degree of acid production by cariogenic bacteria in the dental plaque [16].…”