Effect of Foliar Application of Humic Acid and Zinc Sulfate on Vegetative, Physiological, and Biochemical Characteristics of Physalis alkekengi L. Under Soilless Culture

Kazemi, Samira; Pirmoradi, Mohammad Reza; Karimi, Hamidreza; Raghami, Mahmoud; Rahimi, Asghar; Kheiry, Azizollah; Malekzadeh, M R

doi:10.1007/s42729-023-01305-4

Cited by 3 publications

(2 citation statements)

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“…In the present study, exogenous application of humic acid under stress conditions had a positive effect on the amount of secondary metabolites similar to physios ( Physalis alkekengi L.) 92 . These include phenolic acids, flavonoids, alkaloids and saponins 93 .…”

Section: Discussionsupporting

confidence: 59%

“…Humic acid is known to increase RWC under salinity stress conditions, also evident in the present study and cauliflower ( Brassica oleracea var. Botrytis) 91 , by reducing water evaporation through extensive binding with water molecules and promoting root K + uptake 92 . The use of fig leaf gourd as a rootstock for cucumber under salinity stress, showed a positive effect on water absorption by the roots, leading to an increase in RWC.…”

Section: Discussionmentioning

confidence: 99%

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Humic acid and grafting as sustainable agronomic practices for increased growth and secondary metabolism in cucumber subjected to salt stress

Amerian,

Palangi,

Gohari

et al. 2024

Sci Rep

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Salinity stress poses a significant treat to crop yields and product quality worldwide. Application of a humic acid bio stimulant and grafting onto tolerant rootstocks can both be considered sustainable agronomic practices that can effectively ameliorate the negative effects of salinity stress. This study aimed to assess the above mentioned ameliorative effects of both practices on cucumber plants subjected to saline environments. To attain this goal a factorial experiment was carried out in the form of a completely randomized design with three replications. The three factors considered were (a) three different salinity levels (0, 5, and 10 dS m−1 of NaCl), (b) foliar application of humic acid at three levels (0, 100, and 200 mg L−1), and (c) both grafted and ungrafted plants. Vegetative traits including plant height, fresh and dry weight and number of leaf exhibited a significant decrease under increasing salinity stress. However, the application of humic acid at both levels mitigated these effects compared to control plants. The reduction in relative water content (RWC) of the leaf caused by salinity, was compensated by the application of humic acid and grafting. Thus, the highest RWC (86.65%) was observed in grafting plants with 0 dS m−1 of NaCl and 20 mg L−1 of humic acid. Electrolyte leakage (EL) increased under salinity stress, but the application of humic acid and grafting improved this trait and the lowest amount of EL (26.95%) was in grafting plants with 0 dS m−1 of NaCl and 20 mg L−1 of humic acid. The highest amount of catalase (0.53 mmol H2O2 g−1 fw min−1) and peroxidase (12.290 mmol H2O2 g−1 fw min−1) enzymes were observed in the treatment of 10 dS m−1 of NaCl and 200 mg L−1 humic acid. The highest amount of total phenol (1.99 mg g−1 FW), total flavonoid (0.486 mg g−1 FW), total soluble carbohydrate (30.80 mg g−1 FW), soluble protein (34.56 mg g−1 FW), proline (3.86 µg g−1 FW) was in grafting plants with 0 dS m−1 of NaCl and 200 mg L−1 of humic acid. Phenolic acids and phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) enzymes increased with increasing salinity and humic acid levels. Contrary to humic acid, salt stress increased the sodium (Na+) and chlorine (Cl−) and decreased the amount of potassium (K+) and calcium (Ca2+) in the root and leaf of ungrafted cucumber. However, the application 200 mg L−1 humic acid appeared to mitigate these effects, thereby suggesting a potential role in moderating physiological processes and improving growth of cucumber plants subjected to salinity stress. According to the obtained results, spraying of humic acid (200 mg L−1) and the use of salt resistant rootstocks are recommended to increase tolerance to salt stress in cucumber. These results, for the first time, clearly demonstrated that fig leaf gourd a new highly salt-tolerant rootstock, enhances salt tolerance and improves yield and quality of grafted cucumber plants by reducing sodium transport to the shoot and increasing the amount of compatible osmolytes.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Humic acid and grafting as sustainable agronomic practices for increased growth and secondary metabolism in cucumber subjected to salt stress

Amerian,

Palangi,

Gohari

et al. 2024

Sci Rep

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Comparative Effects of Root and Foliar Leonardite-Suspension Concentrate Application on Plant Growth and Photosynthetic Efficiency of Lettuce Plants (Lactuca sativa L.)

Atero-Calvo,

Magro,

Masetti

et al. 2024

J Plant Growth Regul

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Humic substances (HS) have been defined as a potential plant biostimulant to improve crop yield in a sustainable and environmentally friendly way. Leonardite-suspension concentrate (SC) is a type of HS extracted from lignite that is currently employed to enhance various physiological aspects of plants. However, the different effects between both modes of SC application (root and foliar) are poorly understood, especially on photosynthesis performance. Therefore, this study aimed to investigate the influence of a leonardite-SC-based product (BLACKJAK®), on lettuce growth and photosynthesis efficiency, while comparing both methods of application. For this purpose, four root (R): R1 (0.20 mL/L), R2 (0.40 mL/L), R3 (0.60 mL/L), and R4 (0.80 mL/L), and four foliar: F1 (5.00 mL/L), F2 (7.50 mL/L), F3 (10.00 mL/L), and F4 (12.50 mL/L) BLACKJAK® doses were applied to lettuce plants. Related shoot and root growth parameters, photosynthetic efficiency, and sugar and starch content were assessed in lettuce plants. The results showed that BLACKJAK® improved shoot and root biomass, foliar area, and root length, especially at intermediate doses (R2, R3, F2, and F3), with R3 demonstrating the greatest growth increases. Similarly, the main photosynthetic parameters analyzed (net photosynthetic rate and Rubisco carboxylation efficiency), and the soluble sugars and starch content were improved by the same doses, with R3 showing the best photosynthetic performance. Hence, our study suggests that BLACKJAK® improves lettuce yield and photosynthetic efficiency, particularly with radicular application at R3.

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The effects of humic substances application on the phytohormone profile in Lactuca sativa L.

Atero‐Calvo,

Magro,

Masetti

et al. 2024

Annals of Applied Biology

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Humic substances (HS) are commonly employed as plant biostimulants to enhance crop yields. However, the HS mechanisms of action, as well as the differences between radicular and foliar modes of application, remain unclear. Here, we explored the changes in phytohormonal balance as possible mechanism of HS to enhance lettuce (Lactuca sativa L.) growth, and the difference between both modes of application. For this purpose, BLACKJAK®, a HS‐based product was applied as radicular (R) and foliar (F) at the concentrations (mL/L): 0.20 (R1), 0.40 (R2), 0.60 (R3), 0.80 (R4), 5.00 (F1), 7.50 (F2), 10.00 (F3), and 12.50 (F4). The experiment was performed in pots filled with vermiculite:perlite (3:1) and HS were applied three times with a periodicity of 10 days. Shoot and root growth parameters were measured. In addition, the phythormones indole‐3‐acetic acid (IAA), gibberellins (GAs), trans‐zeatine (tZ), isopentenyl adenine (iP), 1‐aminocyclopropane‐1‐carboxylic acid (ACC), abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA), were analysed by U‐HPLC‐MS. BLACKJAK® application resulted in higher shoot growth at doses R1, R2, R3, F2, and F3, whereas root biomass was increased at R2, R3, F2, F3, and F4, showing radicular better plant growth than foliar applications. Furthermore, HS changed phytohormonal balance in shoots and roots. However, it was with radicular applications, especially at R2, where phytohormonal profile was best associated with plant growth due to the increases observed in IAA, GAs, JA, SA, tZ, and decreased ABA. However, further research is needed to clarify the involvement of hormones in the growth‐promoting action of HS.

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Effect of Foliar Application of Humic Acid and Zinc Sulfate on Vegetative, Physiological, and Biochemical Characteristics of Physalis alkekengi L. Under Soilless Culture

Cited by 3 publications

References 56 publications

Humic acid and grafting as sustainable agronomic practices for increased growth and secondary metabolism in cucumber subjected to salt stress

Humic acid and grafting as sustainable agronomic practices for increased growth and secondary metabolism in cucumber subjected to salt stress

Comparative Effects of Root and Foliar Leonardite-Suspension Concentrate Application on Plant Growth and Photosynthetic Efficiency of Lettuce Plants (Lactuca sativa L.)

The effects of humic substances application on the phytohormone profile in Lactuca sativa L.

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